关键词: 生物燃料, 丙酮丁醇梭菌, 丙酮丁醇发酵, 混合糖, 果糖利用效率

Abstract: Batch acetone-butanol-ethanol （ABE） fermentations from various substrates were briefly introduced. Limiting factors，such as low fructose utilization and butanol production，were involved in batch ABE fermentation by Clostridium acetobutylicum using 55 g/L mixture of glucose and fructose （1∶4） as substrates to stimulate the hydrolysate of Jerusalem artichoke tubers. Then the effect of fructose and glucose with different portions （1∶4 to 1∶2，2∶3，3∶2 and 3∶1） on the performance of batch ABE fermentation was studied. The results showed that the fermentation time was shortened from 76 h to 48 h as the portion increased，and the cell growth with the max OD620 was enhanced from 2.1 to 4.3. The fermentation gave the best metabolic activity of fructose utilization with the glucose/fructose portion of 1∶2，correspondingly，9.7 g/L and 16.0 g/L production of butanol and ABE were produced with only 2.1 g/L residual sugars，resulting in improved fructose utilization efficiency as high as 95.03%.

Key words: biofuel, Clostridium acetobutylicum, acetone-butanol-ethanol fermentation, mixed sugars, fructose utilization efficiency