大豆7S球蛋白与葡聚糖共价键合纳米凝胶的制备与表征

doi:10.11949/j.issn.0438-1157.20160046

化工学报 ›› 2016, Vol. 67 ›› Issue (9): 4020-4026.DOI: 10.11949/j.issn.0438-1157.20160046

• 材料化学工程与纳米技术 • 上一篇

大豆7S球蛋白与葡聚糖共价键合纳米凝胶的制备与表征

冯纪璐, 齐军茹, 翁静宜, 刘倩茹, 曹静, 程萌

华南理工大学食品科学与工程学院, 广东广州 510640

收稿日期:2016-01-11 修回日期:2016-05-06 出版日期:2016-09-05 发布日期:2016-09-05
通讯作者: 齐军茹
基金资助:
国家自然科学基金项目（31370036）；中央高校基本科研业务费专项资金（2015Z2119）。

Preparation and characterization of soy β-conglycinin-dextran nanogels based on Maillard reaction

FENG Jilu, QI Junru, WENG Jingyi, LIU Qianru, CAO Jing, CHENG Meng

School of Food Science and Technology, South China University of Technology, Guangzhou 510640, Guangdong, China

Received:2016-01-11 Revised:2016-05-06 Online:2016-09-05 Published:2016-09-05
Supported by:
supported by the National Natural Science Foundation of China (31370036) and the Fundamental Research Funds for the Central Universities (2015Z2119).

摘要/Abstract

摘要：

基于蛋白质与多糖的Maillard反应与自组装制备一种新型的绿色的具备核壳结构的纳米凝胶。利用干热反应制备大豆7S球蛋白与葡聚糖的共价接枝物（soy β-conglycinin-dextran conjugates，SDC），通过对SDC热处理使其自组装成大豆7S球蛋白-葡聚糖纳米凝胶（soy β-conglycinin-dextran nanogels，SDN），对其形貌、结构及性质进行分析，并利用多糖的空间位阻效应抑制蛋白质宏观过度聚集的理论指导，探讨尺寸均一SDN的形成机制。形貌学与zeta电位分析表明SDN为具备核壳结构的球状粒子，其外壳由亲水性的葡聚糖构成，内核由凝胶化的蛋白构成；表面疏水性分析表明内核蛋白的三级结构发生转变，疏水基团暴露，从而形成多个疏水空腔；稳定性分析表明SDN具有高度的pH稳定性与储存稳定性，对疏水活性物质的输送载体构建具有重要的借鉴意义。

关键词: 大豆7S球蛋白, 葡聚糖, Maillard反应, 自组装, 核壳结构, 纳米凝胶

Abstract:

The novel protein-polysaccharide nanogels were fabricated through a combination of Maillard reaction and self-assembly method, which is facile, safe and green. First, amphiphilic soy β-conglycinin-dextran conjugates (SDC) were prepared by grafting dextran onto soy β-conglycinin via Maillard dry-heating reaction. Then, a heat treatment above the denaturation temperature of protein was used to form soy β-conglycinin-dextran nanogels (SDN). The morphology observation indicated that SDN were of spherical shape with core-shell structures, and the zeta-potential study further verified that soy β-conglycinin was covered with nonionic dextran. The surface hydrophobicity investigation displayed that the conformation of protein was changed and the hydrophobic groups of soy β-conglycinin were exposed to the surface of protein. Therefore, several hydrophobic compartments were formed in the core. The nanogels were pretty stable against long-term storage and pH change. These valuable properties and the low toxicity of nanogels may provide a promising way to deliver hydrophobic compounds.

Key words: soy β-conglycinin, dextran, Maillard reaction, self-assembly, core-shell structure, nanogels

中图分类号:

TS201.7

冯纪璐, 齐军茹, 翁静宜, 刘倩茹, 曹静, 程萌. 大豆7S球蛋白与葡聚糖共价键合纳米凝胶的制备与表征[J]. 化工学报, 2016, 67(9): 4020-4026.

FENG Jilu, QI Junru, WENG Jingyi, LIU Qianru, CAO Jing, CHENG Meng. Preparation and characterization of soy β-conglycinin-dextran nanogels based on Maillard reaction[J]. CIESC Journal, 2016, 67(9): 4020-4026.

参考文献 27

[1]	JOYE I J, MCCLEMENTS D J. Biopolymer-based nanoparticles and microparticles:fabrication, characterization, and application[J]. Current Opinion in Colloid & Interface Science, 2014, 19(5):417-427.
[2]	IKKALA O, TEN BRINKE G. Functional materials based on self-assembly of polymeric supramolecules[J]. Science, 2002, 295(5564):2407-2409.
[3]	WHITESIDES G M, BONCHEVA M. Beyond molecules:self-assembly of mesoscopic and macroscopic components[J]. Proceedings of the National Academy of Sciences, 2002, 99(8):4769-4774.
[4]	WHITESIDES G M, GRZYBOWSKI B. Self-assembly at all scales[J]. Science, 2002, 295(5564):2418-2421.
[5]	SCHMITT C, BOVAY C, VUILLIOMENET A M, et al. Multiscale characterization of individualized β-lactoglobulin microgels formed upon heat treatment under narrow pH range conditions[J]. Langmuir, 2009, 25(14):7899-7909.
[6]	CHEN N, LIN L, SUN W, et al. Stable and pH-sensitive protein nanogels made by self-assembly of heat denatured soy protein[J]. Journal of Agricultural and Food Chemistry, 2014, 62(39):9553-9561.
[7]	HO K M, LI W Y, LEE C H, et al. Mechanistic study of the formation of amphiphilic core-shell particles by grafting methyl methacrylate from polyethylenimine through emulsion polymerization[J]. Polymer, 2010, 51(15):3512-3519.
[8]	TORCHILIN V P. Structure and design of polymeric surfactant-based drug delivery systems[J]. Journal of Controlled Release, 2001, 73(2):137-172.
[9]	WANG D, WANG X. Amphiphilic azo polymers:molecular engineering, self-assembly and photoresponsive properties[J]. Progress in Polymer Science, 2013, 38(2):271-301.
[10]	KATO A, MINAKI K, KOBAYASHI K. Improvement of emulsifying properties of egg white proteins by the attachment of polysaccharide through Maillard reaction in a dry state[J]. Journal of Agricultural and Food Chemistry, 1993, 41(4):540-543.
[11]	WU N N, ZHANG J B, TAN B, et al. Characterization and interfacial behavior of nanoparticles prepared from amphiphilic hydrolysates of β-conglycinin-dextran conjugates[J]. Journal of Agricultural and Food Chemistry, 2014, 62(52):12678-12685.
[12]	LI J, YU S, YAO P, et al. Lysozyme-dextran core-shell nanogels prepared via a green process[J]. Langmuir, 2008, 24(7):3486-3492.
[13]	KANG I, YOON J, LEE Y, et al. Stable vesicle assemblies on surfaces of hydrogel nanoparticles formed from a polysaccharide modified with lipid moieties[J]. Chemical Engineering Journal, 2015, 263:38-44.
[14]	MACKINNON N, GUÉRIN G, LIU B, et al. Liposome-hydrogel bead complexes prepared via biotin-avidin conjugation[J]. Langmuir, 2009, 25(16):9413-9423.
[15]	张曦. 大分子拥挤环境下葡聚糖对大豆7S球蛋白的物性修饰研究[D]. 广州:华南理工大学, 2013. ZHANG X. Soy β-conglycinin modified by dextran in macromolecular crowding condition[D]. Guangzhou:South China University of Technology, 2013.
[16]	ZHANG X, QI J R, LI K K, et al. Characterization of soy β-conglycinin-dextran conjugate prepared by Maillard reaction in crowded liquid system[J]. Food Research International, 2012, 49:648-654.
[17]	WENG J, QI J, YIN S, et al. Fractionation and characterization of soy β-conglycinin-dextran conjugates via macromolecular crowding environment and dry heating[J]. Food Chemistry, 2016, 196:1264-1271.
[18]	NAGANO T, HIROTSUKA M, MORI H, et al. Dynamic viscoelastic study on the gelation of 7S globulin from soybeans[J]. Journal of Agricultural and Food Chemistry, 1992, 40(6):941-944.
[19]	LAEMMLI U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature, 1970, 227(5259):680-685.
[20]	HASKARD C A, LI C E C Y. Hydrophobicity of bovine serum albumin and ovalbumin determined using uncharged (PRODAN) and anionic (ANS-) fluorescent probes[J]. Journal of Agricultural and Food Chemistry, 1998, 46(7):2671-2677.
[21]	KATO A, MIFURU R, MATSUDOMI N, et al. Functional casein-poly saccharide conjugates prepared by controlled dry heating[J]. Bioscience, Biotechnology, and Biochemistry, 1992, 56(4):567-571.
[22]	IWABUCHI S, YAMAUCHI F. Determination of glycinin and beta-conglycinin in soybean proteins by immunological methods[J]. Journal of Agricultural and Food Chemistry, 1987, 35(2):200-205.
[23]	ZHANG K, FANG H, SHEN G, et al. Well-defined cationic shell crosslinked nanoparticles for efficient delivery of DNA or peptide nucleic acids[J]. Proceedings of the American Thoracic Society, 2009, 6(5):450-457.
[24]	ZHOU H, SUN X, ZHANG L, et al. Fabrication of biopolymeric complex coacervation core micelles for efficient tea polyphenol delivery via a green process[J]. Langmuir, 2012, 28:14553-14561.
[25]	YU S, HU J, PAN X, et al. Stable and pH-sensitive nanogels prepared by self-assembly of chitosan and ovalbumin[J]. Langmuir, 2006, 22(6):2754-2759.
[26]	LIN W, GARNETT M C, DAVIES M C, et al. Preparation of surface-modified albumin nanospheres[J]. Biomaterials, 1997, 18(7):559-565.
[27]	HARADA A, KATAOKA K. Novel polyion complex micelles entrapping enzyme molecules in the core:preparation of narrowly-distributed micelles from lysozyme and poly (ethylene glycol)-poly (aspartic acid) block copolymer in aqueous medium[J]. Macromolecules, 1998, 31:288-294.

大豆7S球蛋白与葡聚糖共价键合纳米凝胶的制备与表征

Preparation and characterization of soy β-conglycinin-dextran nanogels based on Maillard reaction

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 27

相关文章 15

编辑推荐

Metrics

本文评价

[1]	陈雅鑫, 袁航, 刘冠章, 毛磊, 杨纯, 张瑞芳, 张光亚. 蛋白质纳米笼介导的酶自固定化研究进展[J]. 化工学报, 2023, 74(7): 2773-2782.
[2]	王杰, 丘晓琳, 赵烨, 刘鑫洋, 韩忠强, 许雍, 蒋文瀚. 聚电解质静电沉积改性PHBV抗氧化膜的制备与性能研究[J]. 化工学报, 2023, 74(7): 3068-3078.
[3]	李勇, 高佳琦, 杜超, 赵亚丽, 李伯琼, 申倩倩, 贾虎生, 薛晋波. Ni@C@TiO₂核壳双重异质结的构筑及光热催化分解水产氢[J]. 化工学报, 2023, 74(6): 2458-2467.
[4]	张浩, 赵宇, 徐志明, 李晋辉. 羧甲基葡聚糖的快速沉降法阻垢特性研究[J]. 化工学报, 2022, 73(4): 1515-1522.
[5]	徐飞翔, 蒋丽群, 郑安庆, 赵增立. 碳基固体酸催化纤维素热解制备左旋葡聚糖和左旋葡萄糖酮[J]. 化工学报, 2022, 73(3): 1166-1172.
[6]	夏青, 徐宇兴, 周运成, 纪雪倩, 冯海兰, 王鹏飞, 谭强强. 核壳结构米粒状FeS₂/C纳米材料制备及储锂性能研究[J]. 化工学报, 2021, 72(5): 2849-2856.
[7]	蒋丽群,岳元茂,徐禄江,钱乐,刘世君,赵增立,李海滨,廖艳芬. 预处理促进木质纤维素快速热解生成左旋葡聚糖[J]. 化工学报, 2021, 72(4): 1825-1832.
[8]	何鹏鹏, 赵颂, 毛晨岳, 王志, 王纪孝. 耐溶剂复合纳滤膜的研究进展[J]. 化工学报, 2021, 72(2): 727-747.
[9]	钱乐,蒋丽群,岳元茂,赵增立. 催化热解生物质生成左旋葡聚糖酮的研究进展[J]. 化工学报, 2020, 71(12): 5376-5387.
[10]	宋刘斌, 蒋鹏, 肖忠良, 周乘风, 黎安娴, 池振振. 核壳结构正极材料界面设计与性能研究[J]. 化工学报, 2019, 70(7): 2426-2438.
[11]	尚良超, 陈晓东, 肖杰. 喷雾干燥颗粒表面形貌形成过程粗粒化模拟[J]. 化工学报, 2019, 70(6): 2153-2163.
[12]	刘皓, 张天巍, 夏登友, 梁强, 王淮斌. 凝胶型核壳结构粉体抑制A类火的有效性研究[J]. 化工学报, 2019, 70(4): 1652-1660.
[13]	潘鑫, 王旭珍, 冯锟, 王爽, 赵宗彬, 邱介山. 碳纳米环：生长机理、可控合成、性质和应用[J]. 化工学报, 2019, 70(10): 3722-3737.
[14]	向文军, 朱朝菊, 刘丹, 周绿山. 分子动力学模拟研究两亲聚合物与疏水纳米粒子自组装核-壳结构[J]. 化工学报, 2019, 70(1): 345-354.
[15]	王凯, 王德武, 侯得印, 袁子怡, 王军. 自组装法制备PVDF-SiO₂/PVSQ超疏水复合膜及膜蒸馏抗污染性能[J]. 化工学报, 2019, 70(1): 298-308.