不同NO3-浓度An/A/O-SBR系统PAOs-GAOs竞争及N2O释放特性

doi:10.11949/0438-1157.20200829

摘要/Abstract

摘要：

采用厌氧/缺氧/好氧运行的序批式生物反应器（An/A/O-SBR），经不同NO₃^-浓度（10，20，30和40 mg/L，以氮计）长期驯化，考察了不同NO₃^-条件下An/A/O-SBR脱氮除磷及N₂O释放特性，基于不同微生物降解特性分析，确定了不同NO₃^-浓度下SBR系统内反硝化聚磷菌（denitrifying phosphorus accumulating organisms，DPAOs）和聚糖菌（glycogen-accumulating organisms， GAOs）竞争关系。结果表明：随NO₃^-浓度增加，总氮（TN）去除率由90%以上降至41.3%，TP去除率呈先增高后降低的趋势，N₂O产率（N₂O_emission/NO_x^-_removal）分别为1.68%、4.17%、8.92%和14.28%。An/A/O-SBR内微生物呈PAOs和GAOs共存的污染物降解特性，高浓度NO₃^-缺氧吸磷过程出现NO₂^-积累，抑制DPAOs活性，GAOs碳源竞争能力增强，NO₃^--N由10 mg/L增至40 mg/L，厌氧阶段PAOs的COD耗量比例由33.5%降至25.1%，相应GAOs的COD耗量由59.3%增至74.1%。DPAOs-GAOs共生体系内，反硝化过程NO₂^-/HNO₂积累耦合反硝化聚糖菌比例增加，加剧了高NO₃^-下An/A/O-SBR内N₂O释放。

关键词: 反硝化聚磷菌, 聚糖菌, 硝态氮, 氧化亚氮, 竞争机制

Abstract:

Using anaerobic/anoxic/aerobic sequencing batch reactors (An/A/O-SBRs), the long-term impact of the NO₃^- cultivation on denitrification phosphorus removal performance and N₂O emission characteristics was investigated in four SBRs of 10(R10), 20(R20), 30(R30) and 40 mg N/L(R40). The anaerobic and anoxic stoichiometric parameters related to various NO₃^- concentrations were monitored over time. The results showed that with the increasing of NO₃^- concentration, the total nitrogen (TN) removal efficiency decreased from more than 90% to 41.3%, and the N₂O emission ratio (N₂O emission/ NO_x^-_removal) were 1.68%, 4.17%, 8.92% and 14.28% respectively, while the total phosphorus removal (TP) efficiency increased at first and then decreased. NO₂^- accumulates in the process of high-concentration NO₃^- anoxic phosphorus absorption, which inhibits the activity of DPAOs, and the carbon source competition ability of GAOs is enhanced. The ratio of DPAOs/COD consumption in anaerobic process decreased from 33.5% to 25.1%, while that of GAOs increased from 59.3% to 74.1% as the NO₃^- increased from 10 to 40 mg N/L. The NO₂^- /HNO₂ accumulation coupled with the increase of GAOs, was the main reason for the reduction of nitrogen and phosphorus efficiencies and higher N₂O yields under higher NO₃^- concentration.

Key words: denitrifying phosphorus accumulating organisms, glycogen accumulating organisms, NO₃^-, N₂O, competitive mechanism

中图分类号:

巩有奎, 李美玲, 孙洪伟. 不同NO₃^-浓度An/A/O-SBR系统PAOs-GAOs竞争及N₂O释放特性[J]. 化工学报, 2021, 72(3): 1675-1683.

GONG Youkui, LI Meiling, SUN Hongwei. The competitive relationship between PAOs-GAOs and N₂O emission in An/A/O-SBR under different NO₃^- cultivation[J]. CIESC Journal, 2021, 72(3): 1675-1683.

图/表 6

图1 试验装置及运行方式

Fig.1 Schematic diagram of the sequence batch reactor(SBR) and its operation mode

图2 An/A/O运行过程中不同NO3--N浓度下氮 (a)及磷（b）变化

Fig.2 Variations of nitrogen (a) and PO43--P (b) under different NO3--N concentration in the An/A/O-SBRs

图3 不同NO3-典型周期内COD、NOx-和PO43--P变化情况

Fig.3 Variations of COD, NOx- and PO43--P during typical cycles under diferent NO3- in An/A/O-SBRs

图4 An/A/O-SBR厌氧阶段COD消耗（a）及内碳源转化（b）

Fig.4 COD consumption (a) and internal polymers variation (b) during anaerobic stage in An/A/O-SBRs

图5 不同NO3-浓度驯化An/A/O-SBR缺氧阶段碳源转化特性

Fig.5 Internal polymers conversion and utilization during anoxic stage in An/A/O-SBR under different NO3-

图6 An/A/O-SBR内缺氧阶段NO2-与溶解态N2O（a）和N2O释放（b）

Fig.6 Characterics of NO2--N, dissolved N2O (a) and N2O emission (b) during anoxic stage in An/A/O-SBRs

参考文献 32

1	Kuba T, van Loosdrecht M C M, Heijnen J J. Phosphorus and nitrogen removal with minimal cod requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system[J]. Water Research, 1996, 30(7): 1702-1710.
2	Ji J, Peng Y, Wang B, et al. A novel SNPR process for advanced nitrogen and phosphorus removal from mainstream wastewater based on anammox, endogenous partial-denitrification and denitrifying dephosphatation[J]. Water Research, 2020, 170: 115363.
3	Larriba O, Rovira-Cal E, Juznic-Zonta Z, et al. Evaluation of the integration of P recovery, polyhydroxyalkanoate production and short cut nitrogen removal in a mainstream wastewater treatment process[J]. Water Research, 2020, 172: 115474.
4	Meng Q, Zeng W, Wang B, et al. New insights in the competition of polyphosphate-accumulating organisms and glycogen- accumulating organisms under glycogen accumulating metabolism with trace Poly-P using flow cytometry[J]. Chemical Engineering Journal, 2020, 385: 123915.
5	Yuan C, Wang B, Peng Y, et al. Enhanced nutrient removal of simultaneous partial nitrification, denitrification and phosphorus removal (SPNDPR) in a single-stage anaerobic/micro-aerobic sequencing batch reactor for treating real sewage with low carbon/ nitrogen[J]. Chemosphere, 2020, 257: 127097.
6	Carvalheira M, Oehmen A, Carvalho G, et al. The effect of substrate competition on the metabolism of polyphosphate accumulating organisms (PAOs)[J]. Water Research, 2014, 64: 149-159.
7	Zhao J, Wang X, Li X, et al. Improvement of partial nitrification endogenous denitrification and phosphorus removal system: balancing competition between phosphorus and glycogen accumulating organisms to enhance nitrogen removal without initiating phosphorus removal deterioration[J]. Bioresource Technology, 2019, 281: 382-391.
8	He Q, Song Q, Zhang S, et al. Simultaneous nitrification, denitrification and phosphorus removal in an aerobic granular sludge sequencing batch reactor with high dissolved oxygen: effects of carbon to nitrogen ratios[J]. Chemical Engineering Journal, 2018, 331: 841-849.
9	Wang X, Zhao J, Yu D, et al. Stable nitrite accumulation and phosphorous removal from nitrate and municipal wastewaters in a combined process of endogenous partial denitrification and denitrifying phosphorus removal (EPDPR)[J]. Chemical Engineering Journal, 2019, 355: 560-571.
10	Fan Z, Zeng W, Wang W, et al. Microbial community at transcription level in the synergy of GAOs and Candidatus Accumulibacter for saving carbon source in wastewater treatment[J]. Bioresource Technology, 2020, 297: 122454.
11	Zhou Y, Pijuan M, Zeng R J, et al. Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge[J]. Environmental Science & Technology, 2008, 42: 8260-8265.
12	Massara T, Malamis M, Guisasola A, et al. A review on nitrous oxide (N₂O) emission during biological nutrient removal from municipal wastewater and sludge reject water[J]. Science of Total Environmental, 2017, 596/597: 106-123.
13	Guo G, Wang Y, Wang C, et al. Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate accumulating organisms linked to phosphorus removal and N₂O production[J]. Frontiers of Environmental Science Engineering, 2013, 7(4): 616-624.
14	Wang Y, Guo G, Wang C, et al. Long-term impact of anaerobic reaction time on the performance and granular characteristics of granular denitrifying biological phosphorus removal systems[J]. Water Research, 2013, 47: 5326 -5337.
15	APHA(American Public Health Association). Standard Methods for the Examination of Water and Wastewater[M]. Baltimore: Port City Press, 1998.
16	Yang Q, Liu X H, Peng C Y, et al. N₂O production during nitrogen removal via nitrite from domestic wastewater: main sources and control method[J]. Environmental Science & Technology, 2009, 43(24): 9400-9406.
17	Oehmen A, Keller-Lehmann B, Zeng R J, et al. Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems[J]. Journal of Chromatography A, 2005, 1070 (1/2): 131-136.
18	Oehmen A, Zeng R J, Yuan Z, et al. Anaerobic metabolism of propionate by polyphosphate-accumulating organisms in enhanced biological phosphorus removal systems[J]. Biotechnology and Bioengineering, 2005, 91(1): 43-53.
19	Cho E, Molof A H. Effect of sequentially combining methanol and acetic acid on the performance of biological nitrogen and phosphorus removal[J]. Journal of Environmental Management, 2004, 73(3): 183-187.
20	Guerrero J, Guisasola A, Baeza J A. Controlled crude glycerol dosage to prevent EBPR failures in C/N/P removal WWTPs[J]. Chemical Engineering Journal, 2015, 271: 114-127.
21	Wang X, Wang S, Zhao J, et al. A novel stoichiometries methodology to quantify functional microorganisms in simultaneous nitrification- endogenous denitrification and phosphorous removal (SNEDPR) [J]. Water Research, 2015, 95: 319-329.
22	蒋轶锋, 朱润晔, 郑建军, 等. 亚硝酸盐为电子受体的反硝化除磷工艺特征[J]. 中国环境科学, 2008, 28(12): 1094-1099.
	Jiang Y F, Zhu R Y, Zheng J J, et al. Process characteristics of denitrifying dephosphatation using nitrite as electron acceptor[J]. China Environmental Science, 2008, 28(12): 1094-1099.
23	张建华, 彭永臻, 张淼, 等. 不同电子受体配比对反硝化除磷特性及内碳源转化利用的影响[J]. 化工学报, 2015, 66(12): 5045-5053.
	Zhang J H, Peng Y Z, Zhang M, et al. Effect of different electron acceptor ratios on removal of nitrogen and phosphorus and conversion and utilization of internal carbon source[J]. CIESC Journal, 2015, 66(12): 5045-5053.
24	Hou H, Wang S, Peng Y, et al. Anoxic phosphorus removal in a pilot scale anaerobic-anoxic oxidation ditch process [J]. Frontiers of Environmental Science & Engineering in China, 2009, 3(1): 106-111.
25	Guisasola A, Qurie M, del Mar Vargas M, et al. Failure of an enriched nitrite-DPAO population to use nitrate as an electron acceptor[J]. Process Biochemistry, 2009, 44(7): 689-695.
26	王晓霞, 王淑莹, 赵骥, 等. SPNED-PR系统内PAOs-GAOs的竞争关系及其氮磷去除特性[J]. 中国环境科学, 2018, 38(2): 551-559.
	Wang X X, Wang S Y, Zhao J, et al. The competitive relationships of PAOs-GAOs in simultaneous partial nitrification- endogenous denitrification and phosphorous removal (SPNED-PR) systems and their nutrient removal characteristics[J]. China Environmental Science, 2018, 38(2): 551-559.
27	Wang Y, Geng J, Ren Z, et al. Effect of anaerobic reaction time on denitrifying phosphorus removal and N₂O production[J]. Bioresource Technology, 2011, 102: 5674-5684.
28	Kerrn-Jespersen J P, Henze M. Biological phosphorus uptake under anoxic and aerobic conditions[J]. Water Research, 1993, 27(4): 617-624.
29	Wei Y, Wang S Y, Ma B, et al. The effect of poly-β-hydroxyalkanoates degradation rate on nitrous oxide production in a denitrifying phosphorus removal system[J]. Bioresource Technology, 2014, 170: 175-182.
30	Welles L, Tian W D, Saad S, et al. Accumulibacter clades Type I and II performing kinetically different glucogen-accumulating organisms metabolisms for anaerobic substrate uptake[J]. Water Research, 2015, 83: 354-366.
31	Lopez-Vazquez C M, Hooijmans C M, Brdjanovic D, et al. A practical method for quantification of phosphorus- and glycogen- accumulating organism populations in activated sludge systems[J]. Water Environmental Research, 2007, 79: 2487-2498.
32	Pan Y, Ni B, Lu H, et al. Evaluating two concepts for the modelling of intermediates accumulation during biological denitrification in wastewater treatment[J]. Water Research, 2015, 71: 21-31.

[1]	蔺彩虹, 王丽, 吴瑜, 刘鹏, 杨江峰, 李晋平. 沸石中碱金属阳离子对CO₂/N₂O吸附分离性能的影响[J]. 化工学报, 2023, 74(5): 2013-2021.
[2]	李媛, 张飞飞, 王丽, 杨江峰, 李立博, 李晋平. MIL-101Cr-F/Cl用于N₂O的捕集研究[J]. 化工学报, 2021, 72(9): 4759-4767.
[3]	巩有奎, 罗佩云, 孙洪伟. 厌氧-限氧SBR处理低C/N生活污水SNDPR启动及N₂O释放[J]. 化工学报, 2021, 72(8): 4381-4390.
[4]	刘小芳, 郭海燕, 张胜男, 黄靓. 聚糖菌反硝化影响因素及内碳源转化特性[J]. 化工学报, 2019, 70(3): 1127-1134.
[5]	王琦, 赵骥, 但琼鹏, 李夕耀, 张琼, 彭永臻. 反硝化聚磷菌的培养富集及处理生活污水的稳定运行[J]. 化工学报, 2019, 70(12): 4828-4834.
[6]	贾淑媛, 王淑莹, 赵骥, 李夕耀, 张琼, 彭永臻. 驯化后的聚糖菌对NO₂^--N和NO₃^--N内源反硝化速率的影响[J]. 化工学报, 2017, 68(12): 4731-4738.
[7]	钱飞跃, 刘小朋, 张念琦, 王晓祎, 王建芳, 刘郭洵. 协同调控C/N负荷提升好氧颗粒污泥亚硝化性能[J]. 化工学报, 2016, 67(9): 3946-3953.
[8]	韩改堂, 乔俊飞, 韩红桂. 基于递归模糊神经网络的污水处理控制方法[J]. 化工学报, 2016, 67(3): 954-959.
[9]	李忠明, 王淑莹, 苗蕾, 曹天昊, 张为堂, 刘文龙, 彭永臻. 单级SBR厌氧/好氧/缺氧处理中期垃圾渗滤液深度脱氮[J]. 化工学报, 2015, 66(2): 746-752.
[10]	董怡君, 王淑莹, 张宇坤, 彭永臻. 高亚硝态氮钝化清洗废水的生物处理及过程控制[J]. 化工学报, 2014, 65(8): 3164-3169.
[11]	张为堂, 薛同来, 彭永臻, 刘青松, 辛振兴, 王淑莹. AAO-BAF反硝化除磷系统的二次启动特性[J]. 化工学报, 2014, 65(2): 658-663.
[12]	巩有奎，王淑莹，王莎莎，王赛. 碳氮比对短程反硝化过程中N₂O产生的影响 [J]. CIESC Journal, 2011, 62(7): 2049-2054.
[13]	冯翠杰，王淑梅，陈少华. 聚磷菌和聚糖菌对碳源竞争及调控的研究进展 [J]. CIESC Journal, 2011, 30(1): 196-.
[14]	刘晖, 孙彦富, 贾晓珊, 周康群, 刘洁萍. 发酵型和非发酵型反硝化聚磷菌的鉴定及代谢机理 [J]. 化工学报, 2010, 61(9): 2454-2462.
[15]	王强, 王继华, 张晓琦, 姜欣欣, 杜丛, 马放. 一株反硝化聚磷菌生长及脱氮除磷特性 [J]. 化工学报, 2010, 61(6): 1540-1544.

不同NO₃^-浓度An/A/O-SBR系统PAOs-GAOs竞争及N₂O释放特性

The competitive relationship between PAOs-GAOs and N₂O emission in An/A/O-SBR under different NO₃^- cultivation

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 32

相关文章 15

编辑推荐

Metrics

本文评价