CIESC Journal ›› 2020, Vol. 71 ›› Issue (2): 766-776.DOI: 10.11949/0438-1157.20190731

• Energy and environmental engineering • Previous Articles     Next Articles

Domestication and identification of cold-resistant bacteria in SBBR reactor

Han WU1(),Ying CHEN1(),Min LIU1,Shuying WANG2,Wei ZHANG3   

  1. 1.College of Architecture and Environment, Sichuan University, Chengdu 610065, Sichuan, China
    2.National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China
    3.State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute at Sichuan University, Chengdu 610065, Sichuan, China
  • Received:2019-07-01 Revised:2019-07-31 Online:2020-02-05 Published:2020-02-05
  • Contact: Ying CHEN

SBBR反应器中耐冷微生物的驯化与识别

吴涵1(),陈滢1(),刘敏1,王淑莹2,张伟3   

  1. 1.四川大学建筑与环境学院,四川 成都 610065
    2.北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室,北京 100124
    3.四川大学高分子研究所高分子材料工程国家重点实验室,四川 成都 610065
  • 通讯作者: 陈滢
  • 作者简介:吴涵(1998—),女,本科生,1483112422@qq.com
  • 基金资助:
    四川大学大学生创新训练计划(C2019104263);城镇污水深度处理与资源化利用技术国家工程实验室开放基金;2018年度中央高校基本科研业务费专项资金

Abstract:

Taking simulated domestic sewage as the treatment object, three different fillers are biofilm carriers, and three SBBRs of A, B and C are operated, and the biofilm of low temperature sewage is domesticated and treated by gradual cooling (25, 20, 15, 10, 6 and 5℃). During the 251 operating cycles, the COD effluent concentration of the three reactors was less than 50 mg/L. The ammonium removal efficiency of the B reactor was better than A and C. At 5℃, the ammonium concentration of effluent of A, B and C were 14.1, 3.79 and 14.1 mg/L, respectively. The results of high-throughput sequencing showed that the dominant microbial species in SBBR were mainly those can degrade organic matter at 5℃. Psychrophilic nitrifying bacteria (Candidatus Nitrotoga) were found only in B reactor. The abundance of other common nitrifying bacteria in the B reactor is higher than which in the A and C reactors. The nitrogen-fixing bacteria (Elstera), which adverse to remove ammonium,has the minimal distribution in the B reactor. These results can explain the difference of the pollutant removal of the three reactors from point of view of microorganism, which shows that B reactor has significant advantages in treating low temperature wastewater.

Key words: waste water, biofilm, low temperature, high throughput sequencing, microbial community structure

摘要:

以模拟生活污水为处理对象,三种不同填料为生物膜载体,运行 A、B、C三个SBBR,通过逐步降温(25、20、15、10、6和5℃)培养驯化处理低温污水的生物膜。运行251周期,三个反应器COD出水浓度均低于50 mg/L;B反应器对氨氮去除效果优于A和C。特别在低温5℃时,A、B和C三个反应器氨氮平均出水浓度分别为14.1、3.79和14.1 mg/L。高通量测序结果显示,5℃时,三个反应器中均以降解有机物的微生物为主;只有B反应器中驯化富集出了嗜冷硝化菌(Candidatus Nitrotoga);而其他有硝化作用的菌属在B反应器中的丰度也高于A和C反应器;不利于脱氮的类固氮菌(Elstera)在A和C反应器中丰度较高。此结果可从微生物角度解释三个反应器处理效果的差异性,表明B反应器处理低温污水有显著优势,其所用填料可结合低温菌进一步开发利用。

关键词: 废水, 生物膜, 低温, 高通量测序, 微生物群落结构

CLC Number: