CIESC Journal ›› 2019, Vol. 70 ›› Issue (12): 4847-4855.DOI: 10.11949/0438-1157.20190753

• Energy and environmental engineering • Previous Articles     Next Articles

Variation of N2O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio

Youkui GONG1,2(),Qiang ZHAO1,Yongzhen PENG2   

  1. 1. Department of Architecture Engineering, Yantai Vocational College, Yantai 264670, Shandong, China
    2. National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China
  • Received:2019-07-02 Revised:2019-08-28 Online:2019-12-05 Published:2019-12-05
  • Contact: Youkui GONG

不同C/N下SBBR脱氮过程N2O释放及胞外多聚物变化

巩有奎1,2(),赵强1,彭永臻2   

  1. 1. 烟台职业学院建筑工程系,山东 烟台 264670
    2. 北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室,北京 100124
  • 通讯作者: 巩有奎
  • 作者简介:巩有奎(1977—),男,博士,副教授,260943813@qq.com
  • 基金资助:
    国家自然科学基金项目(51508008);烟职博士科研(2018002)

Abstract:

At normal temperature of (20±2.0)℃, the characteristics of N2O eimssion and extracellular polymeric substance(EPS) production were studied during simultaneous nitrification and denitrification(SND) process under different C/N ratio (C/N=3.0,5.0, 8.0 and 10.0). When C/N increased from 3.0 to 10.0, the heterotrophic bacteria increased rapidly, DO decreased and the nitrification process was inhibited. The effluent NH4+ increased from below 0.5 mg/L to (7.85±1.42) mg/L, and the N2O emission decreased from (2.68±0.17) mg/L to (1.02±0.12) mg/L. When C/N was 8.0, the maximum total notrogen removal efficiency was 80.4%±3.5%. The accumulation of PHA(poly-β-hydroxyalkanoate) increased firstly and then decreased. Both nitrifier denitrification and heterotrophic denitrification resulted in N2O production. The anoxic area located in the biofilm decreased with the decreasing of C/N ratio, which weakened the denitrification process. With the increase of C/N ratio, N2O diffused into the anoxic region of biofilm can be effectively reduced. The microbial EPS secretion decreased from (57.6±5.6) mg / g VSS to (32.7±3.2) mg / g VSS. The TB-EPS content accounts for 65.8%—68.8% of the total EPS. Under low C/N, the content of polysaccharide (PS) in compact EPS (TB-EPS) increased, the biofilm was more compact, the resistance of N2O diffusion into the anoxic zone increased, and the release increased.

Key words: C/N ratio, simultaneous nitrification and denitrification, N2O, extracellular polymeric substance

摘要:

在(20±2.0)℃条件下,利用序批式生物膜反应器(sequencing batch biofilm reactor,SBBR),考察不同碳氮比(C/N=3.0、5.0、8.0和10.0)下同步脱氮(simultaneous nitrification and denitrification,SND)过程N2O释放及胞外聚合物(extracellular polymeric substance,EPS)变化。C/N由3.0增至10.0,异养菌大量增殖,曝气阶段DO降低,系统硝化性能受到抑制,SBBR系统出水NH4+由0.5 mg/L以下增至(7.85±1.42)mg/L,N2O产量由(2.68±0.17)mg/L降至(1.02±0.12)mg/L。C/N=8.0,TN去除率最大为80.4%±3.5%。反应初期,微生物体内聚β-羟基烷酸酯(PHA)增加,可为后续反硝化过程提供电子供体。AOB好氧反硝化和低氧条件下异养菌反硝化过程均可导致N2O产生。C/N降低,SBBR内部缺氧区域减少,N2O还原过程减弱,释放量增加;C/N增加,N2O扩散进入生物膜内缺氧区域,促进其减量。C/N由3.0增至10.0,微生物EPS分泌由(57.6±5.6)mg / g VSS降至(32.7±3.2)mg / g VSS,其中,TB-EPS含量占65.8%~68.8%。低C/N下,紧密型EPS(TB-EPS)中多糖(PS)含量增加,生物膜更加密实,N2O扩散进入缺氧区阻力增加,释放量增加。

关键词: C/N, 同步硝化反硝化, N2O, 胞外多聚物

CLC Number: