Enhancement of partial nitrification performance of aerobic granular sludge by coordinating C/N loading rate

doi:10.11949/j.issn.0438-1157.20160120

Abstract

Abstract:

In order to start up an autotrophic partial nitrification process, the coordinated control of the influent C/N loading rate was carried out in the lab-scale sequencing batch reactor (SBR) inoculated with heterogeneous granular biomass. During the operation period, the evolution of granule size distribution, extracellular polymeric substances (EPS) composition and dynamics activities of functional microbes were also investigated to elaborate main factors for the conversion of sludge characteristics. The results showed that the nitrite nitrogen (NO₂^--N) accumulation rate of SBR would reach around 1.34 kg·(m³·d)^-1, with the persistent enhancement of ammonia-oxidizing bacteria (AOB) activity and the effective inhibition of nitrite-oxidizing bacteria (NOB), represented by specific removal (μ_NO₃-N) and NO₂^--N accumulation (μ_NO₃-N) rates, respectively. Moreover, the mean diameter of sludge granular increased from 1.4 mm to around 2.2 mm with the color of reddish brown, while its settling ability was improved significantly. Benefited from the accumulation of EPS contents, AOB could be immobilized by granular sludge effectively, even if the settling time of reaction cycle was set at 3 min. In addition, the selective inhibition of NOB caused by both free ammonia (FA) and free nitrous acid (FNA) during the aeration period was the other main factor for stable partial nitrification.

Key words: waste water, granular sludge, aerobic, nitrite accumulation, immobilization, selective inhibition

摘要：

以异养颗粒污泥为接种污泥启动SBR反应器，通过协同调控进水碳、氮负荷比值，成功获得了具备短程亚硝化功能的自养型颗粒污泥。基于对粒径分布、胞外聚合物（EPS）和功能菌动力学活性的分析，系统阐述了影响污泥性状与功能演化的关键因素。结果表明，随着氨氧化菌（AOB）活性（μ_NO₃-N）的持续增强和对亚硝酸盐氧化菌（NOB）活性（μ_NO₃-N）的有效抑制，反应器对亚硝态氮（NO₂^--N）的累积速率可达1.34 kg·（m³·d）^-1。污泥平均粒径由1.4 mm增至2.2 mm，颜色变为红棕色，沉降性能明显改善。得益于EPS的不断累积，颗粒污泥在高选择压条件下（沉淀时间3 min），仍能有效截留、固定AOB。曝气反应期间，游离氨（FA）和游离亚硝酸（FNA）对NOB的选择性抑制也是实现稳定亚硝化反应的重要原因。

关键词: 废水, 颗粒污泥, 需氧, 亚硝态氮累积, 固定化, 选择性抑制

CLC Number:

X703.1

QIAN Feiyue, LIU Xiaopeng, ZHANG Nianqi, WANG Xiaoyi, WANG Jianfang, LIU Guoxun. Enhancement of partial nitrification performance of aerobic granular sludge by coordinating C/N loading rate[J]. CIESC Journal, 2016, 67(9): 3946-3953.

钱飞跃, 刘小朋, 张念琦, 王晓祎, 王建芳, 刘郭洵. 协同调控C/N负荷提升好氧颗粒污泥亚硝化性能[J]. 化工学报, 2016, 67(9): 3946-3953.

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