Preparation and adsorption properties of lysozyme molecularly imprinted polylactide microsphere

doi:10.11949/j.issn.0438-1157.20160176

Abstract

Abstract:

Lysozyme molecularly imprinted modified polylactide microsphere (LZY-MIP-MPLA) was prepared by sol-gel using modified polylactide microsphere as carrier, 2,3-epoxypropoxy propyltrimethoxysilicane as the functional monomer and tetraethyl orthosilicate as crosslinker. The prepared material was characterized by infrared spectroscopy and scanning electron microscopy. The preparation conditions were optimized. The adsorption properties of LZY-MIP-MPLA on lysozyme were studied in detail, and the influence of pH and NaCl on adsorption property was also investigated. The results showed that the adsorption capacity of LZY-MIP-MPLA on lysozyme was better than that of NIP-MPLA, and it needed 200 min to reach the equilibrium. By the analysis of Scatchard equation, the interactions of imprinted cavities and template molecule were not exactly equivalent and there existed two kinds of interaction places. The separation factor of LZY-MIP-MPLA on lysozyme and bovine albumin was 10.13, showing that LZY-MIP-MPLA displayed high recognition ability to the template molecule LZY.

Key words: modified polylactide, lysozyme, molecularly imprinted, enzyme, adsorption, selectivity

摘要：

以改性聚乳酸微球(MPLA)为载体,以γ-(2,3-环氧丙氧)丙基三甲氧基硅烷为功能单体,以四乙氧基硅烷为交联剂,采用溶胶凝胶方法制备了表面溶菌酶分子印迹改性聚乳酸微球(LZY-MIP-MPLA),采用红外、扫描电镜和粒径测定等方法对LZY-MIP-MPLA进行了表征,优化了制备条件。详细研究了LZY-MIP-MPLA对溶菌酶的吸附性能,考察了pH和NaCl对吸附性能的影响。结果显示,LZY-MIP-MPLA对溶菌酶的吸附能力明显大于非印迹改性聚乳酸微球(NIP-MPLA),达到吸附平衡的时间为200min左右。Scatchard方程的分析表明,印迹孔穴对模板分子的作用是不完全等价的,即存在两类不同的结合位点。LZY-MIP-MPLA对溶菌酶和牛血清白蛋白的分离因子为10.13,说明其对溶菌酶具有较好的选择吸附性能。

关键词: 改性聚乳酸, 溶菌酶, 分子印迹, 酶, 吸附, 选择性

CLC Number:

R927.2

TANG Zhimin, MA Xinbin. Preparation and adsorption properties of lysozyme molecularly imprinted polylactide microsphere[J]. CIESC Journal, 2016, 67(6): 2410-2416.

唐志民, 马新宾. 溶菌酶分子印迹聚乳酸微球的制备及吸附性能[J]. 化工学报, 2016, 67(6): 2410-2416.

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