Abstract: Mesostructured cellular foam (MCF) was synthesized and employed as the support for immobilizing lipase LVK-S. The immobilized lipase had been prepared by incorporation of lipase and MCF with 20-30 nm channels in phosphate buffer for 10h, and dried in desiccator following the separating and washing procedures. The preparing immobilized lipase powder was analyzed by IR spectrum for determination of immobilized lipase and an activity of 480 U·g-1 was analyzed by assay of fatty acid releasing rate using pure olive oil as substrate. Prepared waste cooking oil and methnol was mixed with ratio of six in weight, then reaction was initiated by adding 0.2 % (in weight) immobilized lipase powder at 28 °C under stirring condition. Gas chromatograph analysis displayed that there were at least 5 kinds of main FAMEs (Fatty acid methyl ester) containing in broth, and the yield of total FAME to waste cooking oil was about 0.83. Further activity analysis of immobilized lipase using waste cooking oil and pure olive oil as substrate comparatively, indicated that the residual activity was kept over 90 % in pure olive oil reaction system, but the residual activity remained only 61 % in waste cooking oil reaction system. Such phenomenon proved that some lipase activity suppressed elements would exist in waste cooking oil, and removing of such elements in pre-treatment wasted cooking oil was helpful for activity preservation of immobilized lipase in reaction condition.

Key words: immobilized lipase, mesostructured cellular foam, waste cooking oil, biodiesel

关键词: 固载脂肪酶, 介孔泡沫材料, 餐饮废油, 生物柴油