Mechanism of crude oil viscosity reduction by two thermophilic hydrocarbon-degrading bacteria

doi:10.3969/j.issn.0438-1157.2013.11.048

CIESC Journal ›› 2013, Vol. 64 ›› Issue (11): 4240-4245.DOI: 10.3969/j.issn.0438-1157.2013.11.048

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Mechanism of crude oil viscosity reduction by two thermophilic hydrocarbon-degrading bacteria

GAO Peike, WANG Yansen, ZHANG Hongzuo, PAN Xiaoxuan, LI Guoqiang, MA Ting

Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, School of Life Sciences, Nankai University, Tianjin 300071, China

Received:2013-04-11 Revised:2013-07-22 Online:2013-11-05 Published:2013-11-05
Supported by:
supported by the High-tech Research and Development Program of China(2013AA064402)and the National Natural Science Foundation of China(41373074).

两株嗜热解烃菌对原油的降黏机制

高配科, 王燕森, 张宏祚, 潘晓轩, 李国强, 马挺

南开大学生命科学学院, 分子微生物学与技术教育部重点实验室, 天津 300071

通讯作者: 马挺
作者简介:高配科(1987-),男,博士研究生。
基金资助:
国家高技术研究发展计划重点项目(2013AA064402);国家自然科学基金项目(41373074)。

Abstract

Abstract: Two thermophilic hydrocarbon-degrading bacteria,named N12-3 and Z3-14,were isolated from Zhan 3 block,Shengli oilfield.16S rRNA gene sequence analysis indicated that N12-3 belonged to Bacillus sp.and Z3-14 belonged to Geobacillus stearothermophilus.The n-alkane components with carbon number under 20 were preferably degraded by N12-3.After degradation,the content of alkane hydrocarbons,aromatic hydrocarbons and resin decreased by 3.59%,11.24% and 10.08% respectively,while asphalt increased by 26.01%.Furthermore,∑C_21-/∑C₂₂₊ decreased from 2.62 to 0.74.On the other hand,longer n-alkane components were preferably degraded by Z3-14.After degradation,the content of saturated hydrocarbons,aromatic hydrocarbons decreased by 6.8% and 14.39% respectively,while resin and asphalt increased by 13.57% and 1% respectively. Furthermore,∑C_21-/∑C₂₂₊ increased from 1.33 to 3.13.N12-3 could reduce oil viscosity by resin degradation,while Z3-14 could not.However,both strains could reduce oil viscosity by emulsification with bioemulsifiers secreted.The results suggest that microorganisms can improve oil mobility,mainly by emulsification but not by degradation.

Key words: hydrocarbon-degrading bacteria, crude oil, degradation, emulsification, viscosity reduction

关键词: 解烃菌, 原油, 降解, 乳化, 降黏

CLC Number:

Q935
Q939.97

GAO Peike, WANG Yansen, ZHANG Hongzuo, PAN Xiaoxuan, LI Guoqiang, MA Ting. Mechanism of crude oil viscosity reduction by two thermophilic hydrocarbon-degrading bacteria[J]. CIESC Journal, 2013, 64(11): 4240-4245.

高配科, 王燕森, 张宏祚, 潘晓轩, 李国强, 马挺. 两株嗜热解烃菌对原油的降黏机制[J]. 化工学报, 2013, 64(11): 4240-4245.

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Mechanism of crude oil viscosity reduction by two thermophilic hydrocarbon-degrading bacteria

两株嗜热解烃菌对原油的降黏机制

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