Abstract: In order to improve the enzyme conformation for the negative effect of the rigid immobilized enzyme, the hydrophilic and flexible support PS-acyl-P(acrylamide-co-glycidyl methacrylate) with tentacles-like chain containing epoxy group was prepared with the atom transfer radical polymerization (ATRP) method. A series of chain length of tentacles-like support with epoxy group were prepared via changing the proportion of the total monomers to the initiator, and were used for flexible immobilization of organic solvent-tolerant lipase (YCJ01) covalently. The major objective of this paper was to investigate the effect of different lengthes of chain on the activity of immobilized lipase. The longer the chain length (weight gain <3200%) was, the higher the activity of immobilized lipase was.

Key words: atom transfer radical polymerization, tentacles-like support with chain containing epoxy group, length of chain, organic solvent-tolerant lipase, flexible immobilization of enzyme, polymers, biocatalysis

摘要： 为了改善酶的刚性固定化对酶蛋白构象的负面影响，以原子转移自由基聚合法（ATRP）合成了亲水性、柔性、触角状环氧化酶固定化载体PS-acyl-P（AM-co-GMA），通过改变单体总量与引发剂量比例得到不同链长触角状环氧化酶固定化载体，并将其用于耐有机溶剂脂肪酶（YCJ01）的共价柔性固定化，重点考察了链长对固定化酶酶活的影响。结果表明，链长（增重率不超过3200%）越长，酶活越高。

关键词: 原子转移自由基聚合, 触角状环氧化酶固定化载体, 链长, 耐有机溶剂脂肪酶, 酶的柔性固定化, 聚合物, 生物催化