Abstract: Production of 6-aminopenicillanic acid (6-APA) by hydrolysis using penicillin acylase (PA)
was studied as a model of an enzymatic emulsion liquid membrane (ELM) process. The loss of
PA activity was examined for various membrane compositions (organic solvent, surfactant,
carrier). The effects of some experimental variables on the stability of emulsion were
investigated. It was found that the choice of organic solvent greatly affected
tilestability of the emulsion. Increasing the concentration of the carrier in the membrane
phase increases the transfer rate of substrate and products but also has a destabilizing
effect on the emulsion. The recovery of 6-APA obtained by a di-carrier system (N263-N1923)
was much higher than those when either of the di-carriers was used separately.The whole
process was controlled both by the enzymatic reaction rate and by the transfer rate of the
substrate and the products, however, the ratio of them could be changed by varying the
composition of the system. For an optimum condition, it was obtained that the recovery
ratio of 6-APA was over 80% and the conversion of benzyl penicillin (PG) was up to 90% in
the external phase after 30 minutes. Meanwhile, the breakage percentage of the emulsion was
less than 2%.

Key words: 6-aminopenicillanic acid, penicillin, enzymatic bioconversion, emulsion liquid membrane, reactor

摘要： Production of 6-aminopenicillanic acid (6-APA) by hydrolysis using penicillin acylase (PA)
was studied as a model of an enzymatic emulsion liquid membrane (ELM) process. The loss of
PA activity was examined for various membrane compositions (organic solvent, surfactant,
carrier). The effects of some experimental variables on the stability of emulsion were
investigated. It was found that the choice of organic solvent greatly affected
tilestability of the emulsion. Increasing the concentration of the carrier in the membrane
phase increases the transfer rate of substrate and products but also has a destabilizing
effect on the emulsion. The recovery of 6-APA obtained by a di-carrier system (N263-N1923)
was much higher than those when either of the di-carriers was used separately.The whole
process was controlled both by the enzymatic reaction rate and by the transfer rate of the
substrate and the products, however, the ratio of them could be changed by varying the
composition of the system. For an optimum condition, it was obtained that the recovery
ratio of 6-APA was over 80% and the conversion of benzyl penicillin (PG) was up to 90% in
the external phase after 30 minutes. Meanwhile, the breakage percentage of the emulsion was
less than 2%.

关键词: 乳状液膜;反应器;制备;6-APA;生物转化酶;6-氨基青霉烷酸;青霉素;中间产品