Stability of Surfactant-coated Candida Rugosa Lipase in Isooctane

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Stability of Surfactant-coated Candida Rugosa Lipase in Isooctane

SONG Baodong; XING Aihua; WU Jinchuan; WANG Shichang

Chemical Engineering Research Center, School of Chemical Engineering and Technology,
Tianjin University, Tianjin 300072, China

Received:1900-01-01 Revised:1900-01-01 Online:2003-04-28 Published:2003-04-28
Contact: SONG Baodong

表面活性剂包衣Candida rugosa脂肪酶在异辛烷中的稳定性

宋宝东; 邢爱华; 吴金川; 王世昌;

Chemical Engineering Research Center, School of Chemical Engineering and Technology,
Tianjin University, Tianjin 300072, China

通讯作者: 宋宝东

Abstract

Abstract: The stability of Candida rugosa lipase coated with glutamic acid didodecyl ester ribitol
amide was investigated taking esterification of lauryl alcohol and lauric acid in isooctane
as a model reaction. At 30℃, the half-life of the activity of the coated lipase was ca 10
h, the enzyme activity became less changed after 12 h and the residual activity was 39% of
the initial value. The coated lipase obeyed a first-order deactivation model with a
deactivation energy of 29.9 J.mol-1.

Key words: activity, Candida rugosa, lipase, organic solvent, stability, surfactant

摘要： The stability of Candida rugosa lipase coated with glutamic acid didodecyl ester ribitol
amide was investigated taking esterification of lauryl alcohol and lauric acid in isooctane
as a model reaction. At 30℃, the half-life of the activity of the coated lipase was ca 10
h, the enzyme activity became less changed after 12 h and the residual activity was 39% of
the initial value. The coated lipase obeyed a first-order deactivation model with a
deactivation energy of 29.9 J.mol-1.

关键词: activity;Candida rugosa;lipase;organic solvent;stability;surfactant

SONG Baodong, XING Aihua, WU Jinchuan, WANG Shichang . Stability of Surfactant-coated Candida Rugosa Lipase in Isooctane[J]. .

宋宝东, 邢爱华, 吴金川, 王世昌, . 表面活性剂包衣Candida rugosa脂肪酶在异辛烷中的稳定性[J]. CIESC Journal.

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