Abstract: A culture of Lithospermum erythrorhizon adsorbed on fungal cell wall polysaccharides, a
novel bio-adsorbent made from fnngal cell wall, has been established in this paper. Three
steps were involved in this immo-bilization. The first step was preparation of suspended
plant cells from tightly aggregated plant cell clumps. Thedisassembled ratio of 0.715g.g-1
(the disassembled cells over total cells) was obtained under optimum conditionfor the
enzymatic reaction. Then, the adsorption of plant cells onto fungal cell wall
polysaccharides was conductedand the saturated capacity of 12 g cell per gram of carrier
was obtained in adsorption immobilization. Finally, theculture of cells adsorbed on fungal
cell wall polysaccharides was compared with that of cells entrapped in alginateor
suspension cell culture. While exposed to in situ liquid paraffin extraction coupled with
cell culture, the shikoninproductivity of immobilized cells by adsorption was 10.67 g.L-1,
which was 1.8 times of that in suspension cultureand 1.5 times of that entrapped in
alginate.

Key words: bio-adsorbent, immobilization, lithospermum ergthrorhizon

摘要： A culture of Lithospermum erythrorhizon adsorbed on fungal cell wall polysaccharides, a
novel bio-adsorbent made from fnngal cell wall, has been established in this paper. Three
steps were involved in this immo-bilization. The first step was preparation of suspended
plant cells from tightly aggregated plant cell clumps. Thedisassembled ratio of 0.715g.g-1
(the disassembled cells over total cells) was obtained under optimum conditionfor the
enzymatic reaction. Then, the adsorption of plant cells onto fungal cell wall
polysaccharides was conductedand the saturated capacity of 12 g cell per gram of carrier
was obtained in adsorption immobilization. Finally, theculture of cells adsorbed on fungal
cell wall polysaccharides was compared with that of cells entrapped in alginateor
suspension cell culture. While exposed to in situ liquid paraffin extraction coupled with
cell culture, the shikoninproductivity of immobilized cells by adsorption was 10.67 g.L-1,
which was 1.8 times of that in suspension cultureand 1.5 times of that entrapped in
alginate.

关键词: bio-adsorbent;immobilization;lithospermum ergthrorhizon