Abstract: The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive
oil in two-phase system consisting of olive oil and phosphate buffer without organic
solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0,
aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the
coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated
lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the
final residual activity was 27% and 20% of their initial values, respectively. The final
substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.

Key words: Candida rugosa, hydrolysis, lipase, olive oil, solvent-free system, surfactant

摘要： The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive
oil in two-phase system consisting of olive oil and phosphate buffer without organic
solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0,
aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the
coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated
lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the
final residual activity was 27% and 20% of their initial values, respectively. The final
substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.

关键词: Candida rugosa;hydrolysis;lipase;olive oil;solvent-free system;surfactant