Abstract: Experiment was carried out to study the determination of microalgae lipids by solvent extraction with fluorescence spectrometry. In comparison with the gravimetric method，solvent extraction with fluorescence spectrometry is fast，simple，efficient and sensitive. Besides，algal samples can not be stored for a long time, which constrains determination of lipid. Meanwhile, it can measure the content of lipid at a low concentration, which can not be determined by the weighing method when the amount of algae is low. There are good linear relationships between density，dry weight，absorbance and fluorescence intensity of algae. The percentage of microalgae lipids accounting for dry algae weight can be obtained by the use of these linear relationships. Microalgae lipid percentage calculated by solvent extraction with fluorescence spectrometry was 10.45%, while microalgae lipid percentage weighed by the gravimetric method was 8.17%.

Key words: microalgae, lipid, fluorescence spectrometry, absorbance, alkali saponification, acid methyl

摘要： 研究了溶剂提取-荧光测定微藻油脂的一种新的方法。通过与溶剂提取-水浴蒸干称重法比较，表明溶剂提取-荧光光谱法具有快速、简便、高效、灵敏、不受藻样不能长期保存而影响测定制约的特点，可测定藻体量较低时的低浓度油脂含量，而称重法不能测得。利用藻密度、藻干重、藻液吸光度、荧光强度、油脂之间良好的线性关系，通过溶剂提取-荧光光谱法，测得打捞的微藻油脂平均含量为10.45%，溶剂提取-水浴蒸干称重法测得微藻油脂平均含量为8.17%，结果表明溶剂提取-荧光测定法的结果比溶剂提取-水浴蒸干称重法测得值偏大，能更准确地反映真实数值。

关键词: 微藻, 油脂, 荧光光谱法, 吸光度, 碱法皂化, 酸法甲酯化