Abstract: The kinetics of nitrification inhibition，the components of polymeric substances on extracellular and oxygen uptake rate(OUR)of partial nitrification sludge were studied at various concentrations of phenol that was served as short-term toxic inhibitor.The results showed that rate of ammonia nitrification reduced by 37% due to inhibition effect of phenol on the ammonia oxidation bacteria.Under low phenol concentrations(<80 mg·L-1)，it followed Monod’s kinetics for single molecule inhibition，indicating that was a kind of reversible inhibition，and OUR achieved at 20—25 mg O2·L-1·h-1 after the rescission of inhibition.The inhibition constant(52.871 mg·L-1)of mixed sludge system used in this study was far larger than that of an enrichment nitrifier culture.In addition，the phenol promoted the starting of microbial self protection mechanism that could produce more extracellular polymeric substances(EPS)to resist environment change.With the phenol concentrations up to 135 mg·L-1，proteins and carbohydrate in EPS increased up to 87.4% and 311.7% respectively.The ratio of proteins to carbohydrates fell from 22.1 to 3.80 with the increasing initial COD/N.

Key words: partial nitrification, ammonia oxidation bacteria, inhibition kinetics, phenol, extracellular polymeric substance, oxygen uptake rate

摘要： 以苯酚为毒性抑制剂，短程硝化污泥为对象，分析研究了不同苯酚浓度对氨氧化菌硝化过程的抑制特性和抑制动力学，以及对污泥胞外聚合物组分及呼吸速率的短期冲击影响。结果表明，55 mg·L-1苯酚的存在使硝化速率降低37%；低浓度苯酚条件下（< 80 mg·L-1）符合Monod单分子一级动力学方程，且为可逆性抑制，恢复后呼吸速率可达20～25 mg O2·L-1·h-1；且因接种污泥含大量异养菌使得抑制常数（52.871 mg·L-1）远大于纯硝化菌群系统。另外，苯酚的存在促使菌群启动自我保护机制产生更多的胞外聚合物(EPS)抵抗环境变化。苯酚浓度升至135 mg·L-1时，蛋白质和多糖分别增加87.4%和311.7%；而且EPS组分发生相应变化，蛋白质/多糖（P/C）与初始COD/氨氮（C/N）比呈负相关性，P/C从22.1降至3.80。

关键词: 短程硝化, 氨氧化菌, 抑制动力学, 苯酚, 胞外聚合物, 耗氧呼吸速率