Abstract: Absorption and desorption experiments were performed to screen the suitable resin from 6 types of macroporous absorbent resins for the purification of tobacco polyphenol from discarded tobacco leaves. Results indicate that macroporous resin D101 possesses the strongest absorption and desorption capacity. The optimal absorption and desorption condition of macroporous resin D101 is the concentration of feeding solution 2.5 mg/mL, pH value 4.0, feeding rate 2.0 mL/min, elute ethanol concentration 80%, elution rate 2.0 mL/min. The dynamic recovery of tobacco polyphenol was 70.37% under the optimal condition.

Key words: macroporous absorbent resin, discarded tobacco leaves, polyphenol, technology

摘要： 通过吸附和解吸附实验，从6种大孔树脂中筛选出适于分离纯化废次烟叶中烟草多酚的大孔树脂，并确定该大孔树脂纯化工艺参数。结果显示，D101大孔树脂能够较好的富集纯化废次烟叶中的烟草多酚，当提取液浓度为2.5 mg/mL，pH值为4.0，上样液流速为2.0 mL/min，洗脱液为80%乙醇溶液，洗脱液流速为2.0 mL/min时，纯化效果最好，烟草多酚的动态回收率为70.37%。

关键词: 大孔树脂, 废次烟叶, 多酚, 工艺