Abstract: A self-made bioreactor with a nylon cloth cassette immobilized C.roseus plant cells was used tostudy the indole alkaloid production in the“production phase”.The results show this bioreactor provided agood microenvironment for plant cells and was suitable for the culture of C.roseus cells to synthesize the indolealkaloids.A fuzzy multicritical optimal method was used to optimize the constructional and operating parametersof the immobilized plant cell reactor.The fuzzy optimization provide useful information for exploiting thelarge scale alkaloid production process.

Key words: immobilization, reactor, C.roseus, fuzzy mathematical model

摘要： A self-made bioreactor with a nylon cloth cassette immobilized C.roseus plant cells was used tostudy the indole alkaloid production in the“production phase”.The results show this bioreactor provided agood microenvironment for plant cells and was suitable for the culture of C.roseus cells to synthesize the indolealkaloids.A fuzzy multicritical optimal method was used to optimize the constructional and operating parametersof the immobilized plant cell reactor.The fuzzy optimization provide useful information for exploiting thelarge scale alkaloid production process.

关键词: immobilization;reactor;C.roseus;fuzzy mathematical model