Abstract: Novel supermacropore cryogel beads were prepared by combining the inverse-suspension and cryo-polymerization methods under frozen condition. The mean diameter of the beads was 234.1 mm, and the pore size was 10-50 mm. The cation-exchange cryogel beads were prepared by in-situ grafting of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) onto the pore surface of the cryogel beads. The biocompatibility of the cryogel beadswas measured. The adsorption of protein and Cu²⁺ on the cryogel beads was also discussed. The growth of E.coli was little affected when cryogel beads existed in the cultivation. The cryogel beads had stronger adsorption ability after grafting AMPSA, and the adsorption capacity of Cu²⁺ could reach 1.14 mmol·g^-1. Meanwhile, the cryogel beads grafted with AMPSA had the ability of protein adsorption, and the adsorption capacity for lysozyme reached 54.5 mg·g^-1. Such cryogel beads could be used in immobilization of microorganisms, separation of biomass components and adsorption of heavy metal ions.

Key words: porous media, polymers, cryogel beads, ion-exchange, in-situ graft-polymerization, biocompatibility, adsorption

摘要： 利用反相悬浮结合溶剂结晶致孔法制备了具有超大孔隙、球形度良好的聚丙烯酰胺晶胶微球，晶胶微球平均粒径为234.1 mm，孔径约为10～50 mm。采用原位接枝法，将2-丙烯酰胺基-2-甲基-1-丙烷磺酸（AMPSA）接枝到晶胶微球孔隙表面上，得到了带有磺酸基团的阳离子交换晶胶微球。考察了晶胶微球的生物相容性和吸附蛋白质及重金属离子Cu²⁺的性能，结果表明：在大肠杆菌培养液中，添加晶胶微球，对大肠杆菌的生长影响不大。接枝了AMPSA的晶胶微球表现出更强的吸附Cu²⁺的能力，吸附容量达到1.14 mmol·g^-1。同时，接枝后的晶胶微球也具有一定吸附蛋白质的能力，溶菌酶的吸附容量达到54.5 mg·g^-1。因此，该大孔晶胶可望在微生物固定化、生物分离和重金属离子吸附中会发挥更大的作用。

关键词: 多孔介质, 聚合物, 晶胶微球, 离子交换, 原位接枝, 生物相容性, 吸附