Abstract: A series of Al₂O₃@TiO₂ composite supports were prepared and they were covered with a layer of mesoporous TiO₂ by using titanate sol or titania sol. The differences of structures and properties of two TiO₂ samples and their influences on adsorption of bovine serum albumin (BSA) were analyzed. The pore sizes of three alumina supports were 0.37,10.4 and 21.8 mm respectively. The results of XRD and Raman spectroscopy showed that both TiO₂ samples were anatase phase. In addition, N₂ adsorption-desorption isotherms showed that two samples had similar surface area [(100±10) m²·g^-1] and mesoporous structure. Moreover, FESEM image indicated that the morphologies of TiO₂ prepared from potassium titanate sol were affected by pore diameter of Al₂O₃ support. BSA adsorption performance indicated that the adsorption capacity of 20 mm Al₂O₃@TiO₂ composite support prepared with potassium titanate sol (SP20@K-TiO₂) was 22.18 mg·g^-1, higher than the same size composite support prepared with titania sol. The adsorption capacity of TiO₂ in SP20@K-TiO₂ waps 150.88 mg·g^-1. Compared with TiO₂ powder, the BSA adsorption performance of TiO₂ in the composite support was promoted.

Key words: alumina, titania, biological support, composites, protein, adsorption

摘要： 通过钛酸盐和氧化钛溶胶两种制备途径在氧化铝（Al₂O₃）支撑体外包覆介孔氧化钛（TiO₂）层，分析了这两种途径制备的氧化钛表面结构与性质的差异及其对于BSA蛋白吸附性能的影响。以粒径分别为0.37、10.4和21.8 mm的三种氧化铝支撑体作为基材，用XRD、Raman分析了两种路径制备的氧化钛晶型均为锐钛矿相；N₂吸脱附分析其比表面积均在（100±10）m²·g^-1，两者具有相似的介孔结构。FESEM分析发现钛酸钾溶胶制备的氧化钛的形貌受到支撑体孔径的影响。对于在牛血清蛋白（BSA）吸附实验中，钛酸钾溶胶法包覆的20 mm粒径复合载体（SP20@K-TiO₂）吸附量为22.18 mg·g^-1，高于相同条件下氧化钛溶胶制备的Al₂O₃@TiO₂复合载体。SP20@K-TiO₂ 中TiO₂吸附量为150.88 mg·g^-1，相比于TiO₂粉体，复合载体中TiO₂对BSA蛋白吸附的性能得到提升。

关键词: 氧化铝, 二氧化钛, 生物载体, 复合材料, 蛋白质, 吸附