Domestication and identification of cold-resistant bacteria in SBBR reactor

doi:10.11949/0438-1157.20190731

Abstract

Abstract:

Taking simulated domestic sewage as the treatment object, three different fillers are biofilm carriers, and three SBBRs of A, B and C are operated, and the biofilm of low temperature sewage is domesticated and treated by gradual cooling (25, 20, 15, 10, 6 and 5℃). During the 251 operating cycles, the COD effluent concentration of the three reactors was less than 50 mg/L. The ammonium removal efficiency of the B reactor was better than A and C. At 5℃, the ammonium concentration of effluent of A, B and C were 14.1, 3.79 and 14.1 mg/L, respectively. The results of high-throughput sequencing showed that the dominant microbial species in SBBR were mainly those can degrade organic matter at 5℃. Psychrophilic nitrifying bacteria (Candidatus Nitrotoga) were found only in B reactor. The abundance of other common nitrifying bacteria in the B reactor is higher than which in the A and C reactors. The nitrogen-fixing bacteria (Elstera), which adverse to remove ammonium，has the minimal distribution in the B reactor. These results can explain the difference of the pollutant removal of the three reactors from point of view of microorganism, which shows that B reactor has significant advantages in treating low temperature wastewater.

Key words: waste water, biofilm, low temperature, high throughput sequencing, microbial community structure

摘要：

以模拟生活污水为处理对象，三种不同填料为生物膜载体，运行 A、B、C三个SBBR，通过逐步降温（25、20、15、10、6和5℃）培养驯化处理低温污水的生物膜。运行251周期，三个反应器COD出水浓度均低于50 mg/L；B反应器对氨氮去除效果优于A和C。特别在低温5℃时，A、B和C三个反应器氨氮平均出水浓度分别为14.1、3.79和14.1 mg/L。高通量测序结果显示，5℃时，三个反应器中均以降解有机物的微生物为主；只有B反应器中驯化富集出了嗜冷硝化菌（Candidatus Nitrotoga）；而其他有硝化作用的菌属在B反应器中的丰度也高于A和C反应器；不利于脱氮的类固氮菌（Elstera）在A和C反应器中丰度较高。此结果可从微生物角度解释三个反应器处理效果的差异性，表明B反应器处理低温污水有显著优势，其所用填料可结合低温菌进一步开发利用。

关键词: 废水, 生物膜, 低温, 高通量测序, 微生物群落结构

CLC Number:

X 703

Han WU, Ying CHEN, Min LIU, Shuying WANG, Wei ZHANG. Domestication and identification of cold-resistant bacteria in SBBR reactor[J]. CIESC Journal, 2020, 71(2): 766-776.

吴涵, 陈滢, 刘敏, 王淑莹, 张伟. SBBR反应器中耐冷微生物的驯化与识别[J]. 化工学报, 2020, 71(2): 766-776.

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