Application of quinone profile method to indicate structure and activity of functional microbial community in trichloroethylene-contaminated soil

doi:10.11949/0438-1157.20230271

Abstract

Abstract:

This study aimed to evaluate the potential of bioremediation of organic polluted sites using quinone profile method to analyze the activity of functional microbial in trichloroethylene (TCE)-contaminated soil. Microcosm experiments were conducted to investigate the degradation process of TCE in soil and to create a quinone profile. The profile was compared with existing quinone spectral libraries, and a quinone with potential indicator microbial activity for TCE-contaminated soil was selected. By analyzing the changes of the total quinone concentration (TQ) and the concentration of the selected quinone, changes in the active microbial population and functional microbial activity during the degradation process were observed and validated as feasible indicators for reflecting potential microbial activity. The results indicated that primary degradation occurred during 0—50 days, with a degradation rate of TCE over 65% for all three soil groups. Differences in the biodegradation pathways of TCE were observed in the three soil groups. UQ-8, UQ-9, MK-6, and MK-8 were identified as characteristic quinones in TCE-contaminated soil. There were significant positive correlations between TQ, microbial biomass carbon (MTOC), total concentration of characteristic quinones and dehydrogenase activity (P<0.05). Therefore, the quinone profile method can provide an important monitoring method guidance for the monitoring of natural attenuation risk control or the implementation of bioremediation in organically polluted sites.

Key words: organic compounds, contamination, remediation, quinone profile method, microbial activity

摘要：

基于对有机污染场地生物修复潜能评价需求，通过微宇宙试验探讨了醌指纹法解析三氯乙烯（TCE）污染土壤功能微生物活性的潜力。为此，探讨了土壤TCE的降解过程，绘制了土壤醌指纹图谱，通过与现有醌的谱库对比解析了醌指纹图谱并筛选出TCE污染土壤具有指示功能菌群活性潜能特征醌。根据总醌浓度（TQ）和特征醌浓度变化，解析了降解过程中活性微生物量变化和功能菌群活性变化并验证了其可分别作为反映活性微生物量和功能微生物活性潜在指标的可行性。结果表明，主要降解期间在0~50 d， TCE的降解率均在65%以上，三组土壤TCE生物降解途径存在差异；UQ-8、UQ-9、MK-6、MK-8为TCE污染土壤中的特征醌；TQ和微生物量碳（MTOC）、特征醌总浓度与脱氢酶活性均存在显著正相关（P<0.05）。因此，醌指纹法可为有机污染场地监测自然衰减风险管控或生物修复的实施提供重要的监测方法指导。

关键词: 有机化合物, 污染, 修复, 醌指纹法, 微生物活性

CLC Number:

X 53

Lixiang ZHU, Moye LUO, Xiaodong ZHANG, Tao LONG, Ran YU. Application of quinone profile method to indicate structure and activity of functional microbial community in trichloroethylene-contaminated soil[J]. CIESC Journal, 2023, 74(6): 2647-2654.

朱理想, 罗默也, 张晓东, 龙涛, 余冉. 醌指纹法指示三氯乙烯污染土功能微生物活性应用研究[J]. 化工学报, 2023, 74(6): 2647-2654.

Figures/Tables 7

Table 1 Background values for the physical and chemical properties of the three groups of soils

组别	含水率/%		氧化还原电位(ORP)/mV		TCE/(μg/kg)
组别	均值	标准差	均值	标准差	均值	标准差
A	22.5	2.1	-25	2	2.2	1.2
B	26.9	1.2	-10	3	3.1	0.8
C	42.9	1.2	-80	9	4.8	1.3

Fig.1 Variation in TCE concentration in soil

Fig.2 Soil quinone profile vs time

Fig.3 TQ of soil vs time

Fig.4 Relationship between TQ and MTOC for three groups of soils

Fig.5 Soil characteristic quinone total concentration profile vs time

Fig.6 Relationship between total concentration of characteristic quinone and dehydrogenase activity for three groups of soils

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