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收稿日期:
2024-04-23
修回日期:
2024-05-29
出版日期:
2024-06-25
通讯作者:
徐兆超
作者简介:
许宁(1992—),男,博士,ningxu@dicp.ac.cn
基金资助:
Ning XU(), Qinglong QIAO, Zhaochao XU(
)
Received:
2024-04-23
Revised:
2024-05-29
Online:
2024-06-25
Contact:
Zhaochao XU
摘要:
生物荧光标记及成像技术的发展,密切依赖于荧光染料的亮度、光稳定性以及生物相容性等关键发光和化学特性,发展新型荧光团和提升发光性能也将推动生物荧光技术的进步。荧光染料的新颖发光特性又紧密关联于它们的分子结构,而染料结构创新的主要策略包括发现全新的荧光团和对传统荧光团母体进行结构改造。本综述从荧光染料的发光-构效关系切入,详细概述了一系列通过分子结构创新而在发光性能上获得显著提升的荧光染料,并讨论了这些染料在先进生物荧光成像等前沿应用中展现出的卓越表现。文章最后讨论了生物成像领域中荧光染料的发展所面临的挑战。
中图分类号:
许宁, 乔庆龙, 徐兆超. 应用于先进生物成像的荧光染料进展[J]. 化工学报, DOI: 10.11949/0438-1157.20240444.
Ning XU, Qinglong QIAO, Zhaochao XU. Advancements of fluorescent dyes for advanced biological imaging applications[J]. CIESC Journal, DOI: 10.11949/0438-1157.20240444.
图4 Seoul-Fluor染料、CS系列染料以及苯并吡喃鎓-香豆素的探针的化学结构 [36, 38,39]
Fig. 4 Chemical structure of Seoul-Fluor dyes, CS dyes and benzopyranium-coumarin probe [36, 38, 39]
图10 具有自发闪烁性质或光活化性质的代表性荧光团的化学结构[62, 63, 65-68]
Fig. 10 Chemical structures of representative fluorophores with spontaneous blinking and photoactivated properties [62, 63, 65-68]
图11 YL-578、不同N-芳基助色团取代的氟化罗丹明染料和基于N-Ph罗丹明染料的标签蛋白荧光探针的化学结构[69-71]
Fig. 11 Chemical structures of YL-578, fluorinated rhodamine dyes substituted with different N-aryl auxochrome and fluorogenic probes based on N-Ph rhodamine dyes [69-71]
图12 三氟乙胺取代的溶致变色荧光团和RNA探针Nu-AN的化学结构 [72-74]
Fig. 12 Chemical structure of trifluoroethylamine-substituted solvatochromic fluorophores and RNA probe Nu-AN [72-74]
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