线粒体靶向型离子荧光探针的研究进展

doi:10.11949/j.issn.0438-1157.20181398

摘要/Abstract

摘要：

线粒体在细胞的能量代谢过程中发挥着关键作用。线粒体内含有各种生物活性物质，它们在人体各种生理和病理过程中发挥着重要作用。综述了近年来有机单、双光子线粒体靶向型离子荧光探针的研究现状，重点介绍了荧光探针的设计机理、识别机理、特点以及生物应用，并对该类探针的未来发展趋势进行了展望。

关键词: 线粒体, 离子, 荧光探针, 双光子, 选择性, 合成, 成像

Abstract:

Mitochondria play a key role in energy metabolism process of cells. A variety of bio-active species in mitochondria, are of great importance in various physiological and pathological processes. The recent advance in organic one and two-photon mitochondria-targeted fluorescent probes for bio-active ions is reviewed. The design mechanism, recognition mechanism, characteristics and biological applications of the fluorescent probes are emphasized in detail, as well as the developing trends of these probes are prospected.

Key words: mitochondria, ions, fluorescent probes, two-photon, selectivity, synthesis, imaging

中图分类号:

杨志广, 江鑫梅, 程春艳, 朱超胜, 侯志强. 线粒体靶向型离子荧光探针的研究进展[J]. 化工学报, 2019, 70(6): 2060-2074.

Zhiguang YANG, Xinmei JIANG, Chunyan CHENG, Chaosheng ZHU, Zhiqiang HOU. Research progress of mitochondria-targeted fluorescent probes for ions[J]. CIESC Journal, 2019, 70(6): 2060-2074.

图/表 13

图1 探针1和2的分子结构

Fig.1 Structures of probes 1 and 2

图2 探针3~6的分子结构及探针6的活细胞成像图

Fig.2 Structures of probes 3—6 and fluorescent images in living cells of probe 6

图3 探针7~10的分子结构

Fig.3 Structures of probes 7—10

图4 探针11~13的分子结构

Fig.4 Structures of probes 11—13

图5 探针14和15的分子结构

Fig.5 Structures of probes 14 and 15

图6 探针16和17的分子结构及探针17的活细胞成像图

Fig.6 Structures of probes 16 and 17 and fluorescent images in living cells of probe 17

图7 探针18和19的分子结构

Fig.7 Structures of probes 18 and 19

图8 探针20的分子结构以及与K+的响应机理

Fig.8 Structure of probe 20 and its response mechanism to K+

图9 探针21~24的分子结构及探针24的鼠海马切片比率成像图

Fig.9 Structures of probes 21—24 and ratiometric images in rat hippocampal slice of probe 24

图10 探针25~28的分子结构

Fig.10 Structures of probes 25—28

图11 探针29~32的分子结构

Fig.11 Structures of probes 29—32

图12 探针33~35的分子结构及探针35的活细胞成像图

Fig.12 Structures of probes 33—35 and fluorescent images in living cells of probe 35

图13 探针36~39的分子结构

Fig.13 Structures of probes 36—39

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