化工学报 ›› 2024, Vol. 75 ›› Issue (4): 1333-1354.DOI: 10.11949/0438-1157.20231376
收稿日期:
2023-12-26
修回日期:
2024-02-18
出版日期:
2024-04-25
发布日期:
2024-06-07
通讯作者:
徐兆超
作者简介:
江文钞(1994—),男,博士研究生,jiangwc2018@ dicp.ac.cn
基金资助:
Wenchao JIANG1,2(), Zhaochao XU1,2()
Received:
2023-12-26
Revised:
2024-02-18
Online:
2024-04-25
Published:
2024-06-07
Contact:
Zhaochao XU
摘要:
超分辨显微镜提供超越传统光学显微镜衍射极限的成像能力,彻底改变了细胞生物学领域研究。在这一背景下,具有光稳定性、易于修饰和荧光开关可调等独特性能的有机小分子染料获得了新的发展机遇。聚焦于不同细胞器超分辨成像荧光染料,总结了目前可用的超分辨荧光探针的设计和靶向策略。首先简要介绍了三种主要的超分辨成像技术,包括结构光照明显微镜技术、受激发射损耗显微技术和单分子定位成像技术,以及它们对荧光染料性能的不同要求,并介绍了近五年来用于线粒体、溶酶体、细胞膜、脂滴和细胞核的超分辨成像的荧光染料。最后讨论了该领域当前所面临的挑战。
中图分类号:
江文钞, 徐兆超. 细胞器超分辨成像荧光染料[J]. 化工学报, 2024, 75(4): 1333-1354.
Wenchao JIANG, Zhaochao XU. Fluorescent dyes for super-resolution imaging of organelles[J]. CIESC Journal, 2024, 75(4): 1333-1354.
图2 常用细胞器的靶向基团(a)和常用蛋白标签(b)
Fig.2 Ligand groups targeting mitochondria, plasma membrane, lysosomes, and nucleus (a); two universal protein tag ligands (b)
图3 基于正电荷靶向的线粒体超分辨荧光染料结构及其超分辨动态成像
Fig.3 Mitochondrial super-resolution fluorescent dye based on positive charge targeting and its dynamic super-resolution imaging
图4 利用改造的蛋白配体或者蛋白标签实现线粒体可逆标记进行长时间超分辨成像
Fig.4 Using modified Halo-ligands or Halotags to achieve reversible labeling of mitochondria for long-term super-resolution imaging
图5 基于细胞穿透肽靶向的溶酶体染料和商业线粒体染料的双色超分辨动态成像
Fig.5 Two-color super-resolution dynamic imaging of lysosomal and mitochondrial dyes by cell-penetrating peptide-targeted lysosomal dyes and commercial mitochondrial dyes[40]
图6 LysoSR-549的结构及其用于溶酶体运动的动态追踪超分辨成像
Fig.6 The structure of LysoSR-549 and its use for dynamic tracking super-resolution imaging of lysosomal movement[41]
图7 超稳定的溶酶体超分辨染料[42](a)和远红外的溶酶体超分辨染料[43](b)
Fig.7 Ultra-stable lysosomal super-resolution dyes[42] (a) and far-infrared lysosomal super-resolution dyes[43] (b)
图9 细胞膜超分辨荧光染料结构及其3D超分辨动态成像和光谱分辨的超分辨成像
Fig.9 Cell membrane super-resolution fluorescent dyes for 3D super-resolution dynamic imaging and spectral-resolved super-resolution imaging
图11 氢键敏感荧光探针通过缓冲策略对脂滴动态的超分辨稳定成像
Fig.11 Stable super-resolution imaging of lipid droplet (LD) dynamics via a buffering strategy using hydrogen-bond-sensitive fluorescent probes
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