化工学报 ›› 2024, Vol. 75 ›› Issue (4): 1333-1354.DOI: 10.11949/0438-1157.20231376

• 综述与专论 • 上一篇    下一篇

细胞器超分辨成像荧光染料

江文钞1,2(), 徐兆超1,2()   

  1. 1.中国科学院大连化学物理研究所,分离分析化学重点实验室,辽宁 大连 116023
    2.中国科学院大学,北京 100049
  • 收稿日期:2023-12-26 修回日期:2024-02-18 出版日期:2024-04-25 发布日期:2024-06-07
  • 通讯作者: 徐兆超
  • 作者简介:江文钞(1994—),男,博士研究生,jiangwc2018@ dicp.ac.cn
  • 基金资助:
    国家自然科学基金项目(22225806)

Fluorescent dyes for super-resolution imaging of organelles

Wenchao JIANG1,2(), Zhaochao XU1,2()   

  1. 1.CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China
    2.University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2023-12-26 Revised:2024-02-18 Online:2024-04-25 Published:2024-06-07
  • Contact: Zhaochao XU

摘要:

超分辨显微镜提供超越传统光学显微镜衍射极限的成像能力,彻底改变了细胞生物学领域研究。在这一背景下,具有光稳定性、易于修饰和荧光开关可调等独特性能的有机小分子染料获得了新的发展机遇。聚焦于不同细胞器超分辨成像荧光染料,总结了目前可用的超分辨荧光探针的设计和靶向策略。首先简要介绍了三种主要的超分辨成像技术,包括结构光照明显微镜技术、受激发射损耗显微技术和单分子定位成像技术,以及它们对荧光染料性能的不同要求,并介绍了近五年来用于线粒体、溶酶体、细胞膜、脂滴和细胞核的超分辨成像的荧光染料。最后讨论了该领域当前所面临的挑战。

关键词: 荧光染料, 超分辨成像, 荧光探针, 细胞器, 动态

Abstract:

Super-resolution microscopy has revolutionized the field of cell biology by providing enhanced imaging capabilities that surpass the diffraction limit of conventional light microscopy. In this context, organic small molecule dyes with unique properties such as photostability, ease modification, and tunable fluorescence switching have gained new development opportunities. This review focuses on fluorescent dyes for super-resolution imaging of different cellular organelles and summarizes the design and targeting strategies of currently available super-resolution fluorescent probes. The paper begins by briefly introducing three major super-resolution imaging techniques, including structured illumination microscopy, stimulated emission depletion microscopy, and single-molecule localization microscopy, and their diverse requirements for the performance of fluorescent dyes. It also highlights fluorescent dyes used in super-resolution imaging of mitochondria, lysosomes, cell membranes, lipid droplets, and nucleus over the past five years. Finally, the article discusses the future challenges in this field.

Key words: fluorescent dyes, super-resolution imaging, fluorescent probes, organelles, dynamics

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