三种混合模式色谱填料色谱性能的比较

doi:10.11949/j.issn.0438-1157.20160095

化工学报 ›› 2016, Vol. 67 ›› Issue (7): 2893-2900.DOI: 10.11949/j.issn.0438-1157.20160095

三种混合模式色谱填料色谱性能的比较

董彬彬¹, 夏海锋^1,2

1. 江南大学工业生物技术教育部重点实验室, 江苏无锡 214122;
2. 江南大学粮食发酵工艺与技术国家工程实验室, 江苏无锡 214122

收稿日期:2016-01-21 修回日期:2016-04-12 出版日期:2016-07-05 发布日期:2016-07-05
通讯作者: 夏海锋
基金资助:
国家自然科学基金项目（21206054）；江苏省自然科学基金项目（BK20151123）；中央高校基本科研业务费专项资金（JUSRP51401A）。

Comparison of three silica resins in chromatographic property for mixed-mode chromatography

DONG Binbin¹, XIA Haifeng^1,2

1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China;
2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, Jiangsu, China

Received:2016-01-21 Revised:2016-04-12 Online:2016-07-05 Published:2016-07-05
Supported by:
supported by the National Natural Science Foundation of China (21206054), the Natural Science Foundation of Jiangsu Province (BK20151123)and the Fundamental Research Funds for the Central Universities (JUSRP51401A).

摘要/Abstract

摘要：

以γ-缩水甘油醚氧丙基三甲氧基硅烷为中间活化剂，将3-氨基吡啶、2-巯基苯并咪唑和2-巯基-1-甲基咪唑3种杂环配基键合到粒径为5 μm、孔径为30 nm的球形硅胶上，制备3种高效疏水电荷诱导色谱填料。利用溶菌酶、牛血清白蛋白和免疫球蛋白G作为模型蛋白，考察其在3种不同配基色谱柱上的保留行为和分离行为。比较了3种不同性质的模型蛋白在不同色谱柱上的色谱性能的异同。发现以2-巯基-1-甲基咪唑为配基的色谱柱具有良好的pH控制色谱行为和良好的分离性能，初步验证了高效疏水电荷诱导色谱的分离模式及潜在应用。

关键词: 生物分离, 蛋白质, 高效液相色谱, 疏水电荷诱导色谱, 保留因子, 分离行为

Abstract:

Spherical silica gel with a diameter of 5 μm and pore size of 30 nm was activated by KH-560 and coupled with three kinds of ligands (3-aminopyridine, 2-mercapto benzimidazole and 2-mercapto-1-methylimidazole) to prepare the high performance hydrophobic charge-induction chromatography (HPHCIC) column. The chromatographic properties of three columns were compared and evaluated with three model proteins (Lysozyme, BSA and IgG). The column with 2-mercapto-1-methylimidazole as ligand displayed a proper chromatography and separation behavior which controlled by pH. This work demonstrated that the novel synthetic materials can be utilized as the media of high performance hydrophobic charge induction chromatography.

Key words: bioseparation, protein, high performance liquid chromatography, hydrophobic charge-induction chromatography, retention factor, separation behavior

中图分类号:

TP273

董彬彬, 夏海锋. 三种混合模式色谱填料色谱性能的比较[J]. 化工学报, 2016, 67(7): 2893-2900.

DONG Binbin, XIA Haifeng. Comparison of three silica resins in chromatographic property for mixed-mode chromatography[J]. CIESC Journal, 2016, 67(7): 2893-2900.

参考文献 27

[1]	仁斌. 高效液相色谱在医药研究中的应用 [M]. 上海: 科学技术出版社, 2003: 1. REN B. High Performance Liquid Chromatography (HPLC) Application in Medical Research [M]. Shanghai: Scientific and Technical Publisher, 2003: 1.
[2]	KUMAR S, LATHER V, PANDITA D. Stability indicating simplified HPLC method for simultaneous analysis of resveratrol and quercetin in nanoparticles and human plasma [J]. Food Chemistry, 2016, 197: 959-964. DOI: 10.1016/j.foodchem.2015.11.078.
[3]	DING X P, WANG X T, CHEN L L, et al. On-line high-performance liquid chromatography-diode array detection-electrospray ionization-mass spectrometry-chemiluminescence assay of radical scavengers in Epicedium [J]. Journal of Chromatography A, 2011, 1218 (9): 1227-1235. DOI: 10.1016/j.chroma.2011.01.001.
[4]	CEVIKKALP S A, LOKER G B, YAMAN M. A simplified HPLC method for determination of tryptophan in some cereals and legumes [J]. Food Chemistry, 2015, 193 (12): 26-29. DOI: 10.1016/ j.foodchem.2015.02.108.
[5]	RODRIGUEZ-NOGALES J M, CIFUENTES A, GARCIA M C, et al. Characterization of protein fractions from Bt-transgenic and non-transgenic maize varieties using perfusion and monolithic RP-HPLC. Maize differentiation by multivariate analysis [J]. Journal of Agricultural and Food Chemistry, 2006, 55 (10): 3835-3842. DOI: 10.1021/jf063579e.
[6]	WATTANASIRITHAM L, THEERAKULKAIT C, MAIER C. Isolation and identification of antioxidant peptides from enzymatically hydrolyzed rice bran protein [J]. Food Chemistry, 2016, 192 (2): 156-162. DOI: 10.1016/j.foodchem.2015.06.057.
[7]	HUANG X, BARNARD J, SPITZNAGEL T, et al. Protein covalent dimer formation induced by reversed-phase HPLC conditions [J]. Journal of Pharmaceutical Sciences, 2013, 102 (3): 842-851. DOI: 10.1002/jps.23431.
[8]	TEIXID E, MOYANO E, SANTOS F J, et al. Liquid chromatography multi-stage mass spectrometry for the analysis of 5-hydroxymethylfurfural in foods [J]. Journal of Chromatography A, 2008, 1185 (1): 102-108. DOI: 10.1016/j.chroma.2008.01.057.
[9]	KUEHNER D, STAHL M, DEMIRCIOGLU D, et al. From cells to muropeptide structures in 24 h: peptidoglycan mapping by UPLC-MS [J]. Scientific Reports, 2014, 8 (12): 7494. DOI: 10.1038/srep07494.
[10]	GUI F M, LI G C. Development of magnetic molecularly imprinted polymers based on carbon nanotubes-application for trace analysis of pyrethroids in fruit matrices [J]. Journal of Chromatography A, 2014, 1329: 1-9. DOI: 10.1016/j.chroma.2013.12.079.
[11]	MIRA Z, BILJANA J, LJILJANA Z, et al. Application of multicriteria methodology in the development of improved RP-LC-DAD for determination of rizatriptan and its degradation products [J]. Chromatographic, 2008, 68 (11/12): 911-918. DOI: 10.1365/s10337- 008-0823-z.
[12]	BISCHOFF R, MCLAUGHLIN L W. Mixed-mode chromatographic matrices for the resolution of transfer ribonucleic acids [J]. Journal of Chromatography, 1984, 317: 251-61. DOI: 10.1016/S0021- 9673(01)91664-4.
[13]	GUERRIER L, GIROT P, SCHWARTZ W, et al. New method for the selective capture of antibodies under physiological conditions [J]. Bioseparation, 2000, 9 (4): 211-221. DOI: 10.1023/A:1008170226665.
[14]	GHOSE S, HUBBARD B, CRAMER S M. Protein interactions in hydrophobic charge induction chromatography (HCIC) [J]. Biotechnology Progress, 2005, 21 (2): 498-508. DOI: 10.1021/bp049712+.
[15]	BURTON S, HARDING D. Hydrophobic charge induction chromatography: salt independent protein adsorption and facile elution with aqueous buffers [J]. Journal of Chromatography A, 1998, 814 (1/2): 71-81. DOI: 10.1016/S0021-9673(98)00436-1.
[16]	CLAUDIA I, JORMA H, LENNART H, et al. Individuals with selective IgA deficiency resolve rotavirus disease and develop higher antibody titers (IgG, IgG1) than IgA competent individuals [J]. Journal of Medical Virology, 2008, 80 (3): 531-535. DOI: 10.1002/jmv.21101.
[17]	XIA H F, LIN D Q, CHEN Z M, et al. Purification of immunoglobulin of egg yolk with hydrophobic charge induction chromatography comparison of operation modes with packed bed and expanded bed [J]. Separation Science and Technology, 2012, 47 (16): 2366-2372. DOI: 10.1080/01496395.2012.666310.
[18]	COULON D, CABANNE C, FITTON V, et al. Penicillin acylase purification with the aid of hydrophobic charge induction chromatography [J]. Chromatogr. B, 2004, 808: 111-115. DOI: 10.1016/j.jchromb.2004.03.025.
[19]	TAO L, LIN D Q, ZHANG L Q, et al. Characterization of immunoglobulin adsorption on dextran-grafted hydrophobic charge-induction resins: cross-effects of ligand density and pH/salt concentration [J]. Journal of Chromatography A, 2015, 1396: 45-53. DOI: 10.1016/j.chroma.2015.03.074.
[20]	REN J, YAO P, CAO Y M, et al. Application of cyclodextrin-based eluents in hydrophobic charge-induction chromatography: elution of antibody at neutral pH [J]. Journal Chromatogram A, 2014, 1352: 62-68. DOI: 10.1016/j.chroma.2014.05.060.
[21]	XIA H F, LIN D Q, WANG L P, et al. Preparation and evaluation of cellulose adsorbents for hydrophobic charge induction chromatography [J]. Industrial & Engineering Chemistry Research, 2008, 47 (23): 9566-9572. DOI: 10.1021/ie800662r.
[22]	郑梦杰, 夏海锋, 吴婷, 等. 高效疏水电荷诱导色谱介质的基团修饰及其色谱行为的初步研究 [J]. 高校化学工程学报, 2014, (5): 1044-1050. DOI: 1003-9015(2014)28:52.0.TX;2-G. ZHEN M J, XIA H F, WU T, et al. Ligand modification and characteristics of silica gel for high performance hydrophobic charge induction chromatography [J]. Journal of Chemical Engineering of Chinese Universities, 2014, (5): 1044-1050. DOI: 1003-9015(2014)28:52.0.TX;2-G
[23]	XIA H F, LIN D Q, CHEN M Z, et al. Influences of ligand structure and pH on the adsorption with hydrophobic charge induction adsorbents: a case study of antibody IgY [J]. Separation Science and Technology, 2011, 46 (12): 1957-1965. DOI: 10.1080/01496395.2011.579931.
[24]	XIA H F, LIN D Q, CHEN M Z, et al. Salt-promoted adsorption of an antibody onto hydrophobic charge-induction adsorbents [J]. Journal of Chemical & Engineering Data, 2010, 55 (12): 5751-5758. DOI: 10.1021/je100708p.
[25]	GENG X P, GAO H, WANG B H, et al. Thermodynamic analysis of denatured lysozyme folded on moderately hydrophobic surface at 298K [J]. Journal of Thermal Analysis and Calorimetry, 2009, 95 (2): 345-352.DOI: 10.1007/s10973-008-9231-8.
[26]	GENG X P, ZHANG H F, WANG B H, et al. Calorimetric determination of enthalpies of lysozyme folding at a liquid-solid interface [J]. Thermal Analysis & Calorimetry, 2005, 82 (1): 193. DOI: 10.1007/s10973-005-6926-y.
[27]	耿信笃. 计量置换理论及应用 [M]. 北京: 科学出版社, 2004: 25. GENG X D. Measuring the Displacement Theory and Application [M]. Beijing: Science Press, 2004: 25.

三种混合模式色谱填料色谱性能的比较

Comparison of three silica resins in chromatographic property for mixed-mode chromatography

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 27

相关文章 15

编辑推荐

Metrics

本文评价

[1]	赵亚欣, 张雪芹, 王荣柱, 孙国, 姚善泾, 林东强. 流穿模式离子交换层析去除单抗聚集体[J]. 化工学报, 2023, 74(9): 3879-3887.
[2]	陈雅鑫, 袁航, 刘冠章, 毛磊, 杨纯, 张瑞芳, 张光亚. 蛋白质纳米笼介导的酶自固定化研究进展[J]. 化工学报, 2023, 74(7): 2773-2782.
[3]	侯文起, 孙彦, 董晓燕. 碱化修饰甲状腺素运载蛋白显著增强对淀粉样β蛋白聚集的抑制作用[J]. 化工学报, 2023, 74(5): 2100-2110.
[4]	成珊, 罗睿, 田红, 王振琦, 黄经春, 乔瑜. 水热碳化温度对污泥有机氮固液相迁移转化路径影响研究[J]. 化工学报, 2022, 73(11): 5220-5229.
[5]	李南星, 张麟. 靶向于Galectin-10蛋白的哮喘抑制剂设计[J]. 化工学报, 2021, 72(9): 4847-4853.
[6]	于筱溪, 闫真真, 蒋其辉, 吴霞, 张余晓, 王晓娟, 黄方. 溴化1-辛基-3-甲基咪唑聚集状态对蛋白质结晶的影响研究[J]. 化工学报, 2021, 72(9): 4854-4860.
[7]	段凌暄, 姚光晓, 江亮, 王世珍. 耐有机溶剂氨基酸脱氢酶基因挖掘与非天然氨基酸的非水相合成[J]. 化工学报, 2021, 72(7): 3757-3767.
[8]	陈婷婷, 韩恺忻, 陈翠雪, 凌雪萍, 沈亮, 卢英华. 铁还原菌Shewanella xiamenensis BC01的有机溶剂应激研究[J]. 化工学报, 2021, 72(7): 3747-3756.
[9]	范春晖,高宗晔,姚善泾,林东强. 亲和层析介质非特异性吸附的定量表征研究[J]. 化工学报, 2021, 72(10): 5218-5225.
[10]	宋易航, 王楚浩, 方柏山. 胶原酶研究进展与应用[J]. 化工学报, 2019, 70(9): 3213-3227.
[11]	关海晴, 李鸿宇, 李倩, 刘冰南, 王际辉, 王亮. 蛹虫草菌深层液体发酵耦合大孔树脂吸附高效生产虫草素[J]. 化工学报, 2019, 70(7): 2675-2683.
[12]	徐超, 薛誉, 陈虹月, 胡燚. 手性脯氨酸类离子液体化学修饰猪胰脂肪酶催化性能研究[J]. 化工学报, 2019, 70(6): 2221-2228.
[13]	郑禾, 杨盛江, 郑永超, 崔燕, 郭旋, 钟近艺, 周健. 尿素和二甲基亚砜诱导DhaA变性的分子动力学模拟[J]. 化工学报, 2019, 70(11): 4337-4345.
[14]	魏丹, 蒋赣, 苏秋岚, 夏正斌. 牛血清白蛋白初级团聚体分散体在多价反离子作用下的再稳定性和分形结构[J]. 化工学报, 2018, 69(8): 3585-3595.
[15]	梁淼, 余涛, 高翔, 苏荣欣, 齐崴, 何志敏. 基于蛋白及其组装体的金属纳米复合材料构建[J]. 化工学报, 2018, 69(11): 4553-4565.