升降温速率对低温保护剂溶液结晶性质的影响

doi:10.3969/j.issn.0438-1157.2013.08.038

化工学报 ›› 2013, Vol. 64 ›› Issue (8): 2969-2974.DOI: 10.3969/j.issn.0438-1157.2013.08.038

升降温速率对低温保护剂溶液结晶性质的影响

李维杰, 周新丽, 刘宝林, 吕福扣

上海理工大学生物热科学研究所, 上海 200093

收稿日期:2013-01-31 修回日期:2013-03-22 出版日期:2013-08-05 发布日期:2013-08-05
通讯作者: 周新丽
作者简介:李维杰(1983- ),男,博士。
基金资助:
国家自然科学基金项目(50906057,51076108);上海市重点学科项目(S30503)。

Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes

LI Weijie, ZHOU Xinli, LIU Baolin, LÜ Fukou

Institute of Biothermal Technology, University of Shanghai for Science and Technology, Shanghai 200093, China

Received:2013-01-31 Revised:2013-03-22 Online:2013-08-05 Published:2013-08-05
Supported by:
supported by the National Natural Science Foundation of China(50906057,51076108)and Shanghai Leading Academic Discipline Project(S30503).

摘要/Abstract

关键词: 低温显微镜, 降温速率, 升温速率, 结晶, 低温保存

Abstract: When cells are cryopreserved,the morphology of cryoprotectant at crystallization and recrystallization would influence the survival rate of cells.The cryo-microscope system was used to observe the morphology of crystal and sizes of two cryoprotectants during cooling and rewarming at different speeds.It is found that crystals during cooling are smaller and less at higher speed than at lower speed,and the former is favorable for preservation of cells.When the vitrified cryoprotetant is rewarmed,crystals during recrystallization are smaller and less at higher speed than at lower speed,and the former with narrow danger zone is favorable for the survival of oocytes.Besides,high concentration of cryoprotectant is more favorable for the survivalrate of oocytes than lower concentration.

Key words: cryo-microscope, cooling rate, rewarming rate, crystallization, cryopreservation

中图分类号:

TK6
Q599

李维杰, 周新丽, 刘宝林, 吕福扣. 升降温速率对低温保护剂溶液结晶性质的影响[J]. 化工学报, 2013, 64(8): 2969-2974.

LI Weijie, ZHOU Xinli, LIU Baolin, LÜ Fukou. Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes[J]. CIESC Journal, 2013, 64(8): 2969-2974.

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升降温速率对低温保护剂溶液结晶性质的影响

Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes

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