触角状环氧链结构对固定化脂肪酶(YCJ01)酶活的影响

doi:10.3969/j.issn.0438-1157.2014.05.036

化工学报

触角状环氧链结构对固定化脂肪酶(YCJ01)酶活的影响

杨长青¹, 郑涛², 郁晨晨¹, 周俊², 何冰芳¹, 刘晓宁¹

1 南京工业大学生物与制药工程学院, 江苏南京 211816;
2 南京工业大学生物能源研究所, 江苏南京 211816

收稿日期:2013-12-30 修回日期:2014-02-11 出版日期:2014-05-05 发布日期:2014-05-05
通讯作者: 刘晓宁
基金资助:
国家重点基础研究发展计划项目（2013CB733504）；国家高技术研究发展计划项目（2012AA021203）。

Influence of tentacles-like support with chain containing epoxy group on activity of immobilized lipase (YCJ01)

YANG Changqing¹, ZHENG Tao², YU Chenchen¹, ZHOU Jun², HE Bingfang¹, LIU Xiaoning¹

1 School of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China;
2 Bioenergy Research Institute, Nanjing Tech University, Nanjing 211816, Jiangsu, China

Received:2013-12-30 Revised:2014-02-11 Online:2014-05-05 Published:2014-05-05
Supported by:
supported by the National Basic Research Program of China (2013CB733504) and the National High Technology Research and Development Program of China (2012AA021203).

摘要/Abstract

摘要： 为了改善酶的刚性固定化对酶蛋白构象的负面影响，以原子转移自由基聚合法（ATRP）合成了亲水性、柔性、触角状环氧化酶固定化载体PS-acyl-P（AM-co-GMA），通过改变单体总量与引发剂量比例得到不同链长触角状环氧化酶固定化载体，并将其用于耐有机溶剂脂肪酶（YCJ01）的共价柔性固定化，重点考察了链长对固定化酶酶活的影响。结果表明，链长（增重率不超过3200%）越长，酶活越高。

关键词: 原子转移自由基聚合, 触角状环氧化酶固定化载体, 链长, 耐有机溶剂脂肪酶, 酶的柔性固定化, 聚合物, 生物催化

Abstract: In order to improve the enzyme conformation for the negative effect of the rigid immobilized enzyme, the hydrophilic and flexible support PS-acyl-P(acrylamide-co-glycidyl methacrylate) with tentacles-like chain containing epoxy group was prepared with the atom transfer radical polymerization (ATRP) method. A series of chain length of tentacles-like support with epoxy group were prepared via changing the proportion of the total monomers to the initiator, and were used for flexible immobilization of organic solvent-tolerant lipase (YCJ01) covalently. The major objective of this paper was to investigate the effect of different lengthes of chain on the activity of immobilized lipase. The longer the chain length (weight gain <3200%) was, the higher the activity of immobilized lipase was.

Key words: atom transfer radical polymerization, tentacles-like support with chain containing epoxy group, length of chain, organic solvent-tolerant lipase, flexible immobilization of enzyme, polymers, biocatalysis

中图分类号:

O643.3

杨长青, 郑涛, 郁晨晨, 周俊, 何冰芳, 刘晓宁. 触角状环氧链结构对固定化脂肪酶(YCJ01)酶活的影响[J]. 化工学报, DOI: 10.3969/j.issn.0438-1157.2014.05.036.

YANG Changqing, ZHENG Tao, YU Chenchen, ZHOU Jun, HE Bingfang, LIU Xiaoning. Influence of tentacles-like support with chain containing epoxy group on activity of immobilized lipase (YCJ01)[J]. CIESC Journal, DOI: 10.3969/j.issn.0438-1157.2014.05.036.

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触角状环氧链结构对固定化脂肪酶(YCJ01)酶活的影响

Influence of tentacles-like support with chain containing epoxy group on activity of immobilized lipase (YCJ01)

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