氧化还原电位调控混合糖为底物的丁醇发酵

doi:10.3969/j.issn.0438-1157.2014.06.037

化工学报 ›› 2014, Vol. 65 ›› Issue (6): 2225-2231.DOI: 10.3969/j.issn.0438-1157.2014.06.037

氧化还原电位调控混合糖为底物的丁醇发酵

张栩, 吴又多, 齐高相, 刘晨光, 陈丽杰, 白凤武

大连理工大学生命科学与技术学院, 辽宁大连 116024

收稿日期:2013-09-26 修回日期:2013-11-19 出版日期:2014-06-05 发布日期:2014-06-05
通讯作者: 陈丽杰
作者简介:张栩（1988- ），女，硕士研究生。
基金资助:
国家高技术研究发展计划项目（2011AA02A208，2012AA021205），国家自然科学基金项目（21376044）。

Batch butanol fermentation using mixed sugars of glucose and fructose with oxidoreduction potential control

ZHANG Xu, WU Youduo, QI Gaoxiang, LIU Chenguang, CHEN Lijie, BAI Fengwu

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China

Received:2013-09-26 Revised:2013-11-19 Online:2014-06-05 Published:2014-06-05
Supported by:
supported by the National High Technology Research and Development Program of China (2011AA02A208, 2012AA021205) and the National Natural Science Foundation of China (21376044).

摘要/Abstract

摘要： 控制丁醇发酵过程中的氧化还原电位（oxidoreduction potential， ORP）能够大幅提高丁醇产量和果糖利用率，并降低终点有机酸浓度。实验考察了以葡萄糖和果糖混合糖为底物，通过泵入无菌空气控制ORP分别不低于-490、-460、-430及-400 mV丁醇发酵情况。其中，控制ORP不低于-460 mV时，丁醇和总溶剂产量分别达到13.19 g·L^-1及19.71 g·L^-1，相对于不控制ORP的丁醇自然发酵分别提高了139.38%及117.07%，残糖浓度降低至3.20 g·L^-1，糖利用率高达94.18%。该调控策略有效地解决了以葡萄糖和果糖混合糖为底物的丁醇发酵过程中存在的残糖浓度高、丁醇产量低的问题。

关键词: 生物燃料, 丁醇发酵, 生物过程, 氧化还原电位调控, 丙酮丁醇梭菌

Abstract: Butanol production and sugar utilization were greatly improved via oxidoreduction potential (ORP) control strategy during batch acetone-butanol-ethanol (ABE) fermentation with less acids accumulation. Batch fermentations using mixed sugars of glucose and fructose were conducted by pumping sterile air into the fermentor to control ORP above-490,-460,-430 and-400 mV, respectively. When ORP was controlled above-460 mV, butanol production and total solvents reached 13.19 g·L^-1 and 19.71 g·L^-1, respectively, increased by 139.38% and 117.07% compared to the uncontrolled process. At the end of fermentation, residual sugars concentration dropped to 3.2 g·L^-1 and sugar utilization was as high as 94.18%. The results showed that the ORP-control strategy significantly improved sugar utilization, especially for fructose, and butanol production from mixed sugars, indicating that ORP-control could show a stimulatory effect on ABE fermentation and would be an economically competitive strategy for improving fermentation efficiency.

Key words: biofuel, butanol fermentation, bioprocess, ORP-control, Clostridium acetobutylicum

中图分类号:

Q815

张栩, 吴又多, 齐高相, 刘晨光, 陈丽杰, 白凤武. 氧化还原电位调控混合糖为底物的丁醇发酵[J]. 化工学报, 2014, 65(6): 2225-2231.

ZHANG Xu, WU Youduo, QI Gaoxiang, LIU Chenguang, CHEN Lijie, BAI Fengwu. Batch butanol fermentation using mixed sugars of glucose and fructose with oxidoreduction potential control[J]. CIESC Journal, 2014, 65(6): 2225-2231.

参考文献 30

[1]	Lin L, Cunshan Z, Vittayapadung S, et al. Opportunities and challenges for biodiesel fuel [J]. Applied Energy, 2011, 88(4): 1020-1031
[2]	Ma H, Oxley L, Gibson J, et al. A survey of China's renewable energy economy [J]. Renewable and Sustainable Energy Reviews, 2010, 14(1): 438-445
[3]	Durre P. Biobutanol: an attractive biofuel [J]. Biotechnology Journal, 2007, 2: 1525-1534
[4]	Manish Kumar, Kalyan Gayen. Developments in biobutanol production: new insights [J]. Applied Energy, 2011, 88(6): 1999-2012
[5]	Lütke-Eversloh T, Bahl H. Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production [J]. Current Opinion in Biotechnology, 2011, 22(5): 634-647
[6]	Long Xiaohua(隆小华), Liu Zhaopu(刘兆普), Wang Lin(王琳). Effects of seawater irrigation on yield composition and ion distribution of different varieties of helianthus tuberosus in coastal mudflat of semiarid region [J]. Acta Pedologica Sinica(China)(土壤学报), 2007, 44: 300-306
[7]	Niness K R. Inulin and oligofructose: what are they? [J] The Journal of Nutrition, 1999, 129: 1402-1406
[8]	Kays S, Nottingham S. Biology and Chemistry of Jerusalem Artichoke[M]. London: CRC Press, 2007: 1-20
[9]	Kim B H, Bellows P, Datta R, et al. Control of carbon and electron flow in Clostridium acetobutylicum fermentations: utilization of carbon monoxide to inhibit hydrogen production and to enhance butanol yields [J]. Applied and Environmental Microbiology, 1984, 48(4): 764-770
[10]	Peguin S, Goma G, Delorme P, et al. Metabolic flexibility of Clostridium acetobutylicum in response to methyl viologen addition [J]. Applied Microbiology and Biotechnology, 1994, 42: 611-616
[11]	Girbal L, Vasconcelos I, Soucaille P. How neutral red modified carbon and electron flow in Clostridium acetobutylicum grown in chemostat culture at neutral pH [J]. FEMS Microbiology Reviews, 1995, 16: 151-162
[12]	Kim O, Rakesh B, Eugene L I. Redox potential in acetone-butanol fermentations [J]. Applied Biochemistry and Biotechnology, 1988, 18(1): 175-186
[13]	Husson F, Tu V P, Santiago-Gomez M, et al. Effect of redox potential on the growth of Yarrowia lipolytica and the biosynthesis and activity of heterologous hydroperoxide lyase [J]. Journal of Molecular Catalysis B: Enzymatic, 2006, 39(1): 179-183
[14]	De G, Alexeeva S, Snoep J L, et al. The steady-state internal redox state (NADH/NAD) reflects the external redox state and is correlated with catabolic adaptation in Escherichia coli [J]. Journal of Bacteriology, 1999, 181: 2351-2357
[15]	Riondet C, Cachon R, Waché Y, et al. Extracellular oxidoreduction potential modifies carbon and electron flow in Escherichia coli [J]. Journal of Bacteriology, 2000, 182(3): 620-626
[16]	Wang S, Zhang Y, Dong H, et al. Controlling the oxidoreduction potential of the culture of Clostridium acetobutylicum leads to an earlier initiation of solventogenesis, thus increasing solvent productivity [J]. Applied Microbiology and Biotechnology, 2011, 77(5): 1674-1680
[17]	Nakayama S, Bando Y, Ohnishi A, et al. Metabolic engineering for solvent productivity by downregulation of the hydrogenase gene cluster hupCBA in Clostridium saccharoperbutylacetonicum strain N1-4[J]. Applied Microbiology and Biotechnology, 2008, 78:483-493
[18]	Liu C G, Xue C, Lin Y H, et al. Redox potential control and applications in microaerobic and anaerobic fermentations [J]. Biotechnology Advances, 2013.31:257-265
[19]	Li J A, Jiang M, Chen K Q, et al. Effect of redox potential regulation on succinic acid production by Actinobacillus succinogenes [J]. Bioprocess and Biosystems Engineering, 2010, 33(8): 911-920
[20]	Jiang Min(姜岷), Huang Xiumei(黄秀梅), Li Jian(李建). Effect of redox potential regulation on metabolic flux distribution of succinate production by Actinobacillus succinogenes [J]. CIESC Journal(化工学报),2009, 60(10):2555-2561
[21]	Berovic M, Roselj M, Wondra M. Possibilities of redox potential regulation in submerged citric acid bioprocessing on beet molasses substrate [J]. Food Technology and Biotechnology, 2000, 38(3): 193-201
[22]	Sogomonian D, Akopian K, Trchunian A. pH and oxidation-reduction potential change of environment during growth of lactic acid bacteria: effects of oxidizers and reducers [J]. Applied Biochemistry and Microbiology, 2011, 47(1): 33-38
[23]	Kastner J R, Eiteman M A, Lee S A. Effect of redox potential on stationary-phase xylitol fermentations using Candida tropicalis [J]. Applied Microbiology and Biotechnology, 2003, 63(1): 96-100
[24]	Du C, Yan H, Zhang Y, et al. Use of oxidoreduction potential as an indicator to regulate 1,3-propanediol fermentation by Klebsiella pneumonia [J]. Applied Microbiology and Biotechnology, 2006, 69: 554-563
[25]	Wang Na(王娜), Liu Chenguang(刘晨光), Yuan Wenjie(袁文杰). ORP control on very high gravity ethanol fermentation [J]. CIESC Journal(化工学报), 2012, 63(4): 1168-1174
[26]	Liu C G, Lin Y H, Bai F W. Development of redox potential-controlled schemes for very-high-gravity ethanol fermentation [J]. Journal of Biotechnology, 2011, 153(1): 42-47
[27]	Berovi? M. Scale-up of citric acid fermentation by redox potential control [J]. Biotechnology and Bioengineering, 1999, 64(5): 552-557
[28]	Deng Pan(邓攀), Chen Lijie(陈丽杰), Bai Fengwu(白凤武). Acetone-butanol fermentation from the mixture of fructose and glucose [J]. Chinese Journal of Biotechnology(生物工程学报), 2011, 27(10): 1448-1456
[29]	Ounine K, Petitdemange H, Raval G, et al. Regulation and butanol inhibition of D-xylose and D-glucose uptake in Clostridium acetobutylicum [J]. Applied and Environmental Microbiology, 1985, 49: 874-878
[30]	Maddox I S, Steiner E, Hirsch S, et al. The cause of “acid crash” and “acidogenic fermentations” during the batch acetone-butanol-ethanol (ABE) fermentation process [J]. Journal of Molecular Microbiology and Biotechnology, 2000, 2(1): 95-100

氧化还原电位调控混合糖为底物的丁醇发酵

Batch butanol fermentation using mixed sugars of glucose and fructose with oxidoreduction potential control

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