关键词: 层析, 蛋白质, 变性, 复性

Abstract: The impressing from immobilized medium and moving solution might cause protein conformational change and accompanying loss of protein activity during separation by chromatographic processes. At the same time, the denatured protein might refold to gain their native structure and total activity when they pass through the chromatographic column at suitable operation condition. This review focuses on recent advances in column separation and refolding, which have shown promising results on activity recovery from native protein and refolding yield from inclusion bodies. Suitable chromatographic medium and optimized operation system will affect conformational change and improve the yield of active proteins, which are valuable on both laboratory research and commercial productivity.

Key words: 层析, 蛋白质, 变性, 复性