关键词:

大孔载体, 固定化, 脂肪酶

Abstract:

Macroporous poly(glycidyl methacrylate-co-divinylbenzene)carrier containing epoxide group was prepared by bulk polymerization using a multiple porogen consisting of nano-granules of calcium carbonate as solid porogen and the mixture of toluene and heptane as liquid porogen.The results revealed that the carrier, with the porosity of 59.82% and specific surface area of 29.60 m·g-1,mainly contained two kinds of pores, macropores (150—400 nm, approximately 60%) and superpores (2000—8000 nm;about 14.9%).The epoxide groups were hydrolyzed in H+ atmosphere to afford hydroxyl group, which subsequently reacted with glutaraldehyde for the immobilization of lipase.The activities of lipase on different carriers were investigated by catalyzing hydrolysis of olive oil.The result showed that the immobilized lipase on the carrier with double pore structure showed the highest activity of catalyzing esterification of lauric acid.Furthermore, the esterification activity of immobilized lipase was also investigated by using the reaction of n-octanol and dodecanic acid in n-hexane.

Key words:

大孔载体, 固定化, 脂肪酶