ORP对酿酒酵母在木质纤维素水解液抑制物中发酵的影响

doi:10.11949/j.issn.0438-1157.20141318

化工学报 ›› 2015, Vol. 66 ›› Issue (3): 1066-1071.DOI: 10.11949/j.issn.0438-1157.20141318

ORP对酿酒酵母在木质纤维素水解液抑制物中发酵的影响

郝学密, 杜斌, 刘黎阳, 刘晨光, 白凤武

大连理工大学生命科学与技术学院, 辽宁大连 116024

收稿日期:2014-08-28 修回日期:2014-11-30 出版日期:2015-03-05 发布日期:2015-03-05
通讯作者: 刘晨光
基金资助:
国家自然科学基金项目(21406030);中国博士后科学基金特别资助项目(2013T60286);中央高校基本科研业务费专项资金资助项目(DUT14QY19);辽宁省博士科研启动基金项目(20141027)。

Effect of ORP regulation on yeast fermentation with inhibitors of lignocellulose hydrolysate

HAO Xuemi, DU Bin, LIU Liyang, LIU Chenguang, BAI Fengwu

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China

Received:2014-08-28 Revised:2014-11-30 Online:2015-03-05 Published:2015-03-05
Supported by:
supported by the National Natural Science Foundation of China(21406030), the China Postdoctoral Science Special Foundation (2013T60286), the Fundamental Research Funds for Central Universities (DUT14QY19) and the Liaoning Province Doctor Startup Fund (20141027).

摘要/Abstract

摘要：

木质纤维素水解液中的毒性副产物对酿酒细胞具有抑制作用,采用氧化还原电位(oxidoreduction potential, ORP)调控,能够提高细胞对抑制物的耐受性。考察了代表性抑制物乙酸、糠醛及苯酚对酵母细胞生长和代谢的影响,并通过添加氧化剂赤血盐(K₃[Fe(CN)₆])和还原剂二硫苏糖醇(DTT)调节发酵液ORP初始值在(305±5)mV、(157±8)mV及(-150±5)mV,研究不同ORP条件下细胞对抑制物的耐受性影响。结果表明,氧化态能提高生物量并缩短发酵时间。除苯酚外,氧化态也利于提高乙醇的收率。对细胞保护剂甘油合成的方面,氧化态和还原态都会促进甘油合成。在水解液脱毒方面,氧化态表现比还原态要好,因为氧化态促进生物量积累,有利于通过细胞自身代谢脱毒。

关键词: 木质纤维素, 抑制物, 胁迫耐受性, 氧化还原电位, 脱毒

Abstract:

A broad range of inhibitors in hydrolysate of lignocellulose, including weak acids, furan derivatives and phenolic compounds restrain yeast growth and subsequent fermentation. The effect of oxido-reduction potential (ORP) regulation on yeast cell tolerance to the presence of acetic acid, furfural and phenol was investigated. Cell growth and metabolite distribution changed under different ORP levels [(305±5) mV, (157±8) mV and (-150±5) mV] by feeding oxidant (potassium ferricyanide, K₃[Fe(CN)₆]) and reductant (dithiotreitol, DTT). Compared to the control group, cell growth was enhanced, and ethanol yield increased except for phenol group. The yield of glycerol was enhanced under both oxidizing and reducing conditions, since glycerol was a main protective molecule in yeast. High ORP level facilitated detoxification of inhibitors thanks to high biomass accumulation.

Key words: lignocellulose, inhibitors, stress tolerance, ORP, detoxification

中图分类号:

Q815

郝学密, 杜斌, 刘黎阳, 刘晨光, 白凤武. ORP对酿酒酵母在木质纤维素水解液抑制物中发酵的影响[J]. 化工学报, 2015, 66(3): 1066-1071.

HAO Xuemi, DU Bin, LIU Liyang, LIU Chenguang, BAI Fengwu. Effect of ORP regulation on yeast fermentation with inhibitors of lignocellulose hydrolysate[J]. CIESC Journal, 2015, 66(3): 1066-1071.

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ORP对酿酒酵母在木质纤维素水解液抑制物中发酵的影响

Effect of ORP regulation on yeast fermentation with inhibitors of lignocellulose hydrolysate

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