两段式曝气工艺的短程硝化反硝化特性

doi:10.11949/j.issn.0438-1157.20151266

化工学报 ›› 2016, Vol. 67 ›› Issue (4): 1497-1504.DOI: 10.11949/j.issn.0438-1157.20151266

两段式曝气工艺的短程硝化反硝化特性

王盟, 卞伟, 侯爱月, 阚睿哲, 王文啸, 张舒燕, 李军

北京工业大学水质科学与水环境恢复工程北京市重点实验室, 北京 100124

收稿日期:2015-08-07 修回日期:2015-09-21 出版日期:2016-04-05 发布日期:2016-04-05
通讯作者: 李军
基金资助:
国家水体污染控制与治理科技重大专项(2014ZX07201-011);建筑室外水系维护与节水关键技术研究(2013BAJ02B02)。

Characteristics of partial nitrification and denitrification via two-stage aeration

WANG Meng, BIAN Wei, HOU Aiyue, KAN Ruizhe, WANG Wenxiao, ZHANG Shuyan, LI Jun

Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China

Received:2015-08-07 Revised:2015-09-21 Online:2016-04-05 Published:2016-04-05
Supported by:
supported by the National Critical Project for Science and Technology on Water Pollution Prevention and Control of China (2014ZX07201-011).

摘要/Abstract

摘要：

在常温(20~23℃)条件下,采用SBR反应器处理生活污水,通过两段式曝气的方法,控制氨氧化细菌(AOB)的比增长速率高于亚硝酸盐氧化细菌(NOB),经过80个周期的运行实现了短程硝化的快速启动并稳定运行。当COD降解过程结束后,比耗氧速率(SOUR)可以直观反映AOB比增长速率的变化规律。短程硝化实现前后的扫描电镜(SEM)图片表明,在短程硝化实现过程中,污泥中的杆状菌逐渐减少,球状和椭球状细菌逐渐增加。对克隆文库的结果进行分析,发现污泥中的细菌分属8个不同的细菌类群,种类比较丰富,其中优势菌群为β-变形菌和拟杆菌门。

关键词: 短程硝化, 曝气, SBR反应器, 生活污水, 比耗氧速率, 分子生物学

Abstract:

At normal temperature of 20—23℃, the domestic wastewater was treated in a sequencing batch reactor (SBR). The specific growth rate of ammonia oxidizing bacteria (AOB) was kept higher than nitrite oxidizing bacteria (NOB) via the two-stage aeration. After 80 cycles, the rapid startup of the partial nitrification was realized and operated steadily. The results showed that the specific oxygen uptake rate (SOUR) could reflect the variation of the specific growth rate of AOB when chemical oxygen demand (COD) had been largely degraded. Analyzing the scanning electron microscopy (SEM) images in different periods, it indicated that the quantity of bacillus was decreased and the quantity of spherical or elliptic spherical bacteria was increased. The establishment of 16S rDNA clone library indicated that the species of bacteria were rich and the microbes belonged to eight different groups of bacteria. Moreover, the β-Proteobacteria and Bacteroidetes were the dominant bacterial groups.

Key words: partial nitrification, aeration, sequencing batch reactor, domestic wastewater, specific oxygen uptake rate, molecular biology

中图分类号:

X703.5

王盟, 卞伟, 侯爱月, 阚睿哲, 王文啸, 张舒燕, 李军. 两段式曝气工艺的短程硝化反硝化特性[J]. 化工学报, 2016, 67(4): 1497-1504.

WANG Meng, BIAN Wei, HOU Aiyue, KAN Ruizhe, WANG Wenxiao, ZHANG Shuyan, LI Jun. Characteristics of partial nitrification and denitrification via two-stage aeration[J]. CIESC Journal, 2016, 67(4): 1497-1504.

参考文献 27

[1]	WU J, YAN G, ZHOU G, et al. Model predictive control of biological nitrogen removal via partial nitrification at low carbon/nitrogen (C/N) ratio[J]. Journal of Environmental Chemical Engineering, 2014, 2 (4): 1899-1906.
[2]	KORNAROS M, DOKIANAKIS S N, LYBERATOS G. Partial nitrification/denitrification can be attributed to the slow response of nitrite oxidizing bacteria to periodic anoxic disturbances[J]. Environmental Science &Technology, 2010, 44 (19): 7245-7253.
[3]	WU J, XU T, JIANG X, et al. Model based optimization of partial nitrification by monitoring nitrous oxide(N2O) emission[J]. Journal of Environmental Chemical Engineering, 2015, 3 (3): 1602-1613.
[4]	CHEN Y, WANG Y, FAN M, et al. Preliminary study of shortcut nitrification and denitrification using immobilized of mixed activated sludge and denitrifying sludge[J]. Procedia Environmental Sciences, 2011, 11: 1171-1176.
[5]	WANG J, PENG Y, WANG S, et al. Nitrogen removal by simultaneous nitrification and denitrification via nitrite in a sequence hybrid biological reactor[J]. Chinese Journal of Chemical Engineering, 2008, 16 (5): 778-784.
[6]	TOKUTOMI T. Operation of a nitrite-type airlift reactor at low DO concentration[J]. Water Science and Technology, 2004, 49 (5/6): 81-88.
[7]	VAN KEMPEN R, MULDER J W, UIJTERLINDE C A, et al. Overview: full scale experience of the SHARON® process for treatment of rejection water of digested sludge dewatering[J]. Water Science and Technology, 2001, 44 (1): 145-152.
[8]	LAANBROEK H J, GERARDS S. Competition for limiting amounts of oxygen between Nitrosomonas europaea and Nitrobacter winogradskyi grown in mixed continuous cultures[J]. Archives of Microbiology, 1993, 159 (5): 453-459.
[9]	CIUDAD G, GONZALEZ R, BORNHARDT C, et al. Modes of operation and pH control as enhancement factors for partial nitrification with oxygen transport limitation[J]. Water Research, 2007, 41 (20): 4621-4629.
[10]	GUO J, PENG Y, HUANG H, et al. Short-and long-term effects of temperature on partial nitrification in a sequencing batch reactor treating domestic wastewater[J].Journal of Hazardous Materials, 2010, 179 (1/2/3): 471-479.
[11]	MARTINS A M, PAGILLA K, HEIJNEN J J, et al. Filamentous bulking sludge—a critical review[J]. Water Research, 2004, 38 (4): 793-817.
[12]	CIUDAD G, RUBILAR O, MUNOZ P, et al. Partial nitrification of high ammonia concentration wastewater as a part of a shortcut biological nitrogen removal process[J]. Process Biochemistry, 2005, 40 (5): 1715-1719.
[13]	GARRIDO J M, VAN BENTHUM W, VAN LOOSDRECHT M, et al. Influence of dissolved oxygen concentration on nitrite accumulation in a biofilm airlift suspension reactor[J]. Biotechnology and Bioengineering, 1997, 53 (2): 168-178.
[14]	丁文川, 吴丹, 曾晓岚, 等. 不同曝气量对SBBR短程硝化微生物特性及氮转化的影响[J]. 环境科学学报, 2012, 32 (9): 2112-2118. DING W C, WU D, ZENG X L, et al. Effect of aeration flow on microbial characteristics and nitrogen transformation of shortcut nitrification processing in a sequencing batch biofilm reactor[J]. Acta Scientiae Circumstantiae, 2012, 32 (9): 2112-2118.
[15]	李凌云, 彭永臻, 李论, 等. 活性污泥系统比耗氧速率在线检测与变化规律[J]. 化工学报, 2010, 61 (4): 995-1000. LI L Y, PENGY Z, LI L, et al. On-line determination and variations of specific oxygen uptake rate in activated sludge system[J]. CIESC Journal, 2010, 61 (4): 995-1000.
[16]	郑林雪, 李军, 胡家玮, 等. 同步硝化反硝化系统中反硝化细菌多样性研究[J]. 中国环境科学, 2015, 35 (1): 116-121 ZHENG L X, LI J, HU J W, et al. Analysis of denitrifying bacteria community composition in simultaneous nitrification and denitrification systems[J]. China Environmental Science, 2015, 35 (1): 116-121
[17]	卢阳阳. 生活污水同步硝化反硝化脱氮研究[D]. 北京: 北京交通大学, 2014. LU Y Y. The study on simultaneous nitrification and denitrification of domestic sewage[D]. Beijing: Beijing Jiaotong University, 2014.
[18]	李凌云. 基于AOB种群优化和耗氧速率在线检测的短程硝化研究[D]. 北京: 北京工业大学, 2011. LI L Y. AOB regulation and on-line determination of specific oxygen rate for partial nitrification[D]. Beijing: Beijing University of Technology, 2011.
[19]	李凌云, 彭永臻,杨庆, 等. SBR工艺短程硝化快速启动条件的优化[J]. 中国环境科学, 2009, 29 (3): 312-317. LI L Y, PENG Y Z, YANG Q, et al. Factors optimization of rapid start-up for partial nitrification in SBR process[J]. China Environmental Science, 2009, 29 (3): 312-317.
[20]	苏东霞,李冬,张肖静, 等. 曝停时间比对间歇曝气SBR短程硝化的影响[J]. 中国环境科学, 2014, 34(5): 1152-1158. SU D X, LI D, ZHANG X J, et al. Effects of different ratios of aeration time and anaerobic time on shortcut nitrification in the intermittent aeration SBR[J]. China Environmental Science, 2014, 34 (5): 1152-1158.
[21]	李冬, 陶晓晓, 李占, 等. SBR亚硝化快速启动过程中影响因子研究[J]. 环境科学, 2011, 32 (8): 2317-2322. LI D, TAO X X, LI Z, et al. Factors of the rapid startup for nitrosation in sequencing batch reactor[J]. Environmental Science, 2011, 32 (8): 2317-2322.
[22]	郑雅楠,滝川哲夫,郭建华, 等. SBR法常、低温下生活污水短程硝化的实现及特性[J]. 中国环境科学, 2009, 29 (9): 935-940. ZHENG Y,AKIO T, GUO J H, et al. Partial nitrification via nitrite at ordinary and low temperatures in an SBR treating domestic wastewater[J]. China Environmental Science, 2009, 29 (9): 935-940.
[23]	孙洪伟, 王淑莹, 王希明, 等. 低温SBR反硝化过程亚硝态氮积累试验研究[J]. 环境科学, 2009, 30 (12): 3619-3623. SUN H W, WANG S Y, WANG X M, et al. Nitrite accumulation during the denitration process in SBR at low temperature[J]. Environmental Science, 2009, 30 (12): 3619-3623.
[24]	周丹丹, 马放, 董双石, 等. 溶解氧和有机碳源对同步硝化反硝化的影响[J]. 环境工程学报, 2007, 1 (4): 25-28. ZHOU D D, MA F, DONG S S, et al. Influences of DO and organic carbon on simultaneous nitrification and denitrification[J]. Chinese Journal of Environmental Engineering, 2007, 1 (4): 25-28.
[25]	郭建华, 王淑莹, 郑雅楠, 等. 实时控制实现短程硝化过程中种群结构的演变[J]. 哈尔滨工业大学学报, 2010, 42 (8): 1259-1263. GUO J H, WANG S Y,ZHENG Y N, et al. Assessment of partial nitrification achieved by real-time aeration duration control through microbial population shift using FISH and SEM[J]. Journal of Harbin Institute of Technology, 2010, 42 (8): 1259-1263.
[26]	AMANN R I, LUDWIG W, SCHLEIFER K. Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J]. Microbiological Reviews, 1995, 59 (1): 143-169.
[27]	赵光. 两段式厌氧工艺产甲烷发酵特性及微生物生态调控机制研究[D]. 哈尔滨: 哈尔滨工业大学, 2013. ZHAO G. Performance of methane production in two-stage anaerobic digestion and microbial ecological regulation mechanisms[D]. Harbin: Harbin Institute of Technology, 2013.

两段式曝气工艺的短程硝化反硝化特性

Characteristics of partial nitrification and denitrification via two-stage aeration

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