SRT对富集高聚PHA能力嗜盐MMC的影响

doi:10.11949/j.issn.0438-1157.20151644

化工学报 ›› 2016, Vol. 67 ›› Issue (6): 2575-2582.DOI: 10.11949/j.issn.0438-1157.20151644

SRT对富集高聚PHA能力嗜盐MMC的影响

崔有为, 林小媛, 冀思远, 施云鹏

北京工业大学环境与能源工程学院, 北京 100124

收稿日期:2015-11-02 修回日期:2016-02-29 出版日期:2016-06-05 发布日期:2016-06-05
通讯作者: 崔有为
基金资助:
国家自然科学基金项目(51478011,51178004);北京市自然科学基金项目(8132013);北京市属高等学校高层次人才引进与培养计划项目。

Influence of SRT on enriching halophilic MMC with capacity of PHA storage

CUI Youwei, LIN Xiaoyuan, JI Siyuan, SHI Yunpeng

College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China

Received:2015-11-02 Revised:2016-02-29 Online:2016-06-05 Published:2016-06-05
Supported by:
supported by the National Natural Science Foundation of China (51478011, 51178004), the Natural Science Foundation of Beijing (8132013) and the Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions.

摘要/Abstract

摘要：

调控菌群富集过程的工艺参数,富集具有高PHA积累能力的混合菌(MMC)是发酵生产PHA的第一步也是最重要的一步。采用批式培养条件,集中研究了污泥停留时间(SRT)对PHA积累菌群的富集作用。研究结果表明SRT影响富集MMC的底物降解速率(q_S),微生物积累PHA的速率(q_P)和PHA积累能力。长SRT(SRT4d)导致了较低的q_S,q_P和PHA细胞含量;过短的SRT(SRT1d)则降低了MMC的PHA转化率(Y_PHA/S)和PHA积累能力,刺激了非PHA积累菌群的快速增殖。研究确定的最佳SRT为2d,在此条件下富集的MMC最大PHA积累量可达细胞干重的56%。研究证明了SRT在选择嗜盐MMC中的重要作用,为快速富集PHA积累能力MMC奠定基础。

关键词: SRT, PHA积累菌, 聚合物, 发酵, 生物工程

Abstract:

Enriching mixed microbial culture (MMC) with capacity of PHA storage via controlling the enrichment process parameters is the first and the most important step for the fermentation production of PHA. This paper elucidated the role of sludge retention time (SRT) in the enrichment process under batch culture conditions. The results showed that SRT influenced the specific substrate degradation rate (q_S), specific PHA accumulation rate (q_P) and the PHA storage capacity of enriched MMC. The long SRT (SRT 4 d) led to the decreased q_S, q_P and PHA accumulation ability. The short SRT reduced the PHA conversion rate (Y_PHA/S) and PHA accumulation ability of MMC. The optimal SRT was determined as 2 d for enriched MMC in this study, which presented 56% cell dry weight PHA content. The study proved that the SRT played the important role in enriching halophilic MMC, which laid the foundation on enriching PHA-storage MMC rapidly.

Key words: SRT, PHA storage bacteria, polymers, fermentation, biological engineering

中图分类号:

X703

崔有为, 林小媛, 冀思远, 施云鹏. SRT对富集高聚PHA能力嗜盐MMC的影响[J]. 化工学报, 2016, 67(6): 2575-2582.

CUI Youwei, LIN Xiaoyuan, JI Siyuan, SHI Yunpeng. Influence of SRT on enriching halophilic MMC with capacity of PHA storage[J]. CIESC Journal, 2016, 67(6): 2575-2582.

参考文献 20

[1]	崔有为, 彭永臻, 李晶, 等. 盐度抑制下的MUCT处理效能及其微生物种群变化 [J]. 环境科学, 2009, (2): 488-492. CUI Y W, PENG Y Z, LI J, et al. Salt effects on treatment performance and microbial community structure in MUCT [J]. Environmental Sciences, 2009, (2): 488-492.
[2]	LITCHFIELD C D. Potential for industrial products from the halophilic Archaea[J]. Journal of Industrial Microbiology & Biotechnology, 2011, 38 (10): 1635-1647.
[3]	GAO X, CHEN J, WU Q, et al. Polyhydroxyalkanoates as a source of chemicals, polymers, and biofuels [J]. Current Opinion in Biotechnology, 2011, 22 (6): 768-774.
[4]	QUILLAGUAMAN J, GUZMAN H, VAN-THUOC D, et al. Synthesis and production of polyhydroxyalkanoates by halophiles: current potential and future prospects [J]. Applied Microbiology and Biotechnology, 2010, 85 (6): 1687-1696.
[5]	TAN G, CHEN C L, LI L, et al. Start a research on biopolymer polyhydroxyalkanoate (PHA): a review [J]. Polymers, 2014, 6 (3): 706-754.
[6]	GOBI K, VADIVELU V M. Aerobic dynamic feeding as a strategy for in situ accumulation of polyhydroxyalkanoate in aerobic granules [J]. Bioresource Technology, 2014, 161: 441-445.
[7]	JOHNSON K, JIANG Y, KLEEREBEZEM R, et al. Enrichment of a mixed bacterial culture with a high polyhydroxyalkanoate storage capacity [J].Biomacromolecules, 2009, 10 (4): 670-676.
[8]	MOROLEJO-GARATE H, PALMEIRO-SANCHEZ T, KLEEREBEZEM R, et al. Influence of the cycle length on the production of PHA and polyglucose from glycerol by bacterial enrichments in sequencing batch reactors [J]. Biotechnology and Bioengineering, 2013, 110 (12): 3148-3155.
[9]	ALBUQUERQUE M, EIROA M, TORRES C, et al. Strategies for the development of a side stream process for polyhydroxyalkanoate (PHA) production from sugar cane molasses [J]. Journal of Biotechnology, 2007, 130 (4): 411-421.
[10]	JOHNSON K, KLEEREBEZEM R, VAN LOOSDRECHT M. Influence of the C/N ratio on the performance of polyhydroxybutyrate (PHB) producing sequencing batch reactors at short SRTs [J]. Water Research, 2010, 44 (7): 2141-2152.
[11]	SERAFIM L S, LEMOS P C, OLIVEIRA R. Optimization of polyhydroxybutyrate production by mixed cultures submitted to aerobic dynamic feeding conditions [J]. Biotechnology and Bioengineering, 2004, 87 (2): 145-160.
[12]	MARANG L, JIANG Y, VAN LOOSDRECHT M C M, et al. Butyrate as preferred substrate for polyhydroxybutyrate production [J]. Bioresource Technology, 2013, 142: 232-239.
[13]	BARROCAL V M, TERESA GARCIA-CUBERO M, GONZALEZ-BENITO G, et al. Production of biomass by Spirulina maxima using sugar beet vinasse in growth media [J]. New Biotechnology, 2010, 27 (6): 851-856.
[14]	CUI Y W, DING J R, JI S Y, et al. Start-up of halophilic nitrogen removal via nitrite from hypersaline wastewater by estuarine sediments in sequencing batch reactor [J]. International Journal of Environmental Science and Technology, 2014, 11 (2): 281-292.
[15]	OEHMEN A, KELLER-LEHMANN B, ZENG R J, et al. Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems [J]. Journal of Chromatography A, 2005, 1070 (1/2): 131-136.
[16]	APHA. Standard Methods for the Examination of Water and Wastewater [M]. New York: American Public Health Association, 1995.
[17]	崔有为, 张宏宇, 冀思远, 等. 容积负荷变化模式对嗜盐混合菌发酵乙酸合成PHB的影响 [J]. 化工学报, 2015, 66 (10): 4177-4184. DOI: 10.11949/j.issn.0438-1157.20150287. CUI Y W, ZHANG H Y, JI S Y, et al. Influence of organic loading rate change modes on PHB production by halophilic sludge fermenting acetate acid [J].CIESC Journal, 2015, 66 (10): 4177-4184. DOI: 10.11949/j.issn.0438-1157.20150287.
[18]	ALBUQUERQUE M G E, TORRES C A V, REIS M A M. Polyhydroxyalkanoate (PHA) production by a mixed microbial culture using sugar molasses: effect of the influent substrate concentration on culture selection [J]. Water Research, 2010, 44 (11): 3419-3433.
[19]	DIONISI D, BECCARI M, DI GREGORIO S, et al. Storage of biodegradable polymers by an enriched microbial community in a sequencing batch reactor operated at high organic load rate [J]. Journal of Chemical Technology and Biotechnology, 2005, 80 (11): 1306-1318.
[20]	MORALEJO-GARATE H, KLEEREBEZEM R, MOSQUERA-CORRAL A, et al. Impact of oxygen limitation on glycerol-based biopolymer production by bacterial enrichments [J]. Water Research, 2013, 47 (3): 1209-1217.

SRT对富集高聚PHA能力嗜盐MMC的影响

Influence of SRT on enriching halophilic MMC with capacity of PHA storage

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 20

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王杰, 丘晓琳, 赵烨, 刘鑫洋, 韩忠强, 许雍, 蒋文瀚. 聚电解质静电沉积改性PHBV抗氧化膜的制备与性能研究[J]. 化工学报, 2023, 74(7): 3068-3078.
[2]	刘杰, 吴立盛, 李锦锦, 罗正鸿, 周寅宁. 含乙烯基胺酯键聚醚类可逆交联聚合物的制备及性能研究[J]. 化工学报, 2023, 74(7): 3051-3057.
[3]	高学金, 姚玉卓, 韩华云, 齐咏生. 基于注意力动态卷积自编码器的发酵过程故障监测[J]. 化工学报, 2023, 74(6): 2503-2521.
[4]	龙臻, 王谨航, 任俊杰, 何勇, 周雪冰, 梁德青. 离子液体协同PVCap抑制天然气水合物生成实验研究[J]. 化工学报, 2023, 74(6): 2639-2646.
[5]	杨琴, 秦传鉴, 李明梓, 杨文晶, 赵卫杰, 刘虎. 用于柔性传感的双形状记忆MXene基水凝胶的制备及性能研究[J]. 化工学报, 2023, 74(6): 2699-2707.
[6]	陈韶云, 徐东, 陈龙, 张禹, 张远方, 尤庆亮, 胡成龙, 陈建. 单层聚苯胺微球阵列结构的制备及其吸附性能[J]. 化工学报, 2023, 74(5): 2228-2238.
[7]	张建华, 陈萌萌, 孙雅雯, 彭永臻. 部分短程硝化同步除磷耦合Anammox实现生活污水高效脱氮除磷[J]. 化工学报, 2023, 74(5): 2147-2156.
[8]	龙臻, 王谨航, 何勇, 梁德青. 离子液体与动力学抑制剂作用下混合气体水合物生成特性研究[J]. 化工学报, 2023, 74(4): 1703-1711.
[9]	吴学红, 栾林林, 陈亚南, 赵敏, 吕财, 刘勇. 可降解柔性相变薄膜的制备及其热性能[J]. 化工学报, 2023, 74(4): 1818-1826.
[10]	吕阳光, 左培培, 杨正金, 徐铜文. 三嗪框架聚合物膜用于有机纳滤甲醇/正己烷分离[J]. 化工学报, 2023, 74(4): 1598-1606.
[11]	罗来明, 张劲, 郭志斌, 王海宁, 卢善富, 相艳. 1~5 kW高温聚合物电解质膜燃料电池堆的理论模拟与组装测试[J]. 化工学报, 2023, 74(4): 1724-1734.
[12]	刘海芹, 李博文, 凌喆, 刘亮, 俞娟, 范一民, 勇强. 羟基-炔点击化学改性半乳甘露聚糖薄膜的制备及性能研究[J]. 化工学报, 2023, 74(3): 1370-1378.
[13]	徐东, 田杜, 陈龙, 张禹, 尤庆亮, 胡成龙, 陈韶云, 陈建. 聚苯胺/二氧化锰/聚吡咯复合纳米球的制备及其电化学储能性[J]. 化工学报, 2023, 74(3): 1379-1389.
[14]	刘润竹, 储甜甜, 张孝阿, 王成忠, 张军营. α，ω-端羟基亚苯基氟硅聚合物的合成及性能[J]. 化工学报, 2023, 74(3): 1360-1369.
[15]	胡月, 马守骏, 蹇锡高, 翁志焕. 新型聚芳醚腈固化邻苯二甲腈树脂的研究[J]. 化工学报, 2023, 74(2): 871-882.