CIESC Journal ›› 2021, Vol. 72 ›› Issue (5): 2413-2425.DOI: 10.11949/0438-1157.20201327
• Reviews and monographs • Previous Articles Next Articles
MAO Jinzhu1,3(),XIAO Shuling1,2,YANG Zhichun1,2,WANG Xiaoyu1,4,ZHANG Shi1,5,CHEN Junhong1,2,XIE Jisheng1,2,CHEN Fude1,2,HUANG Zinuo1,2,FENG Tianyu1,2,ZHANG Aihui1,2,6(),FANG Baishan1,2,6,7()
Received:
2020-09-17
Revised:
2020-12-23
Online:
2021-05-05
Published:
2021-05-05
Contact:
ZHANG Aihui,FANG Baishan
毛金竹1,3(),肖淑玲1,2,杨智淳1,2,王孝宇1,4,张诗1,5,陈俊宏1,2,谢佶晟1,2,陈福德1,2,黄子诺1,2,冯天宇1,2,张瑷珲1,2,6(),方柏山1,2,6,7()
通讯作者:
张瑷珲,方柏山
作者简介:
毛金竹(1999—),女,硕士研究生,CLC Number:
MAO Jinzhu, XIAO Shuling, YANG Zhichun, WANG Xiaoyu, ZHANG Shi, CHEN Junhong, XIE Jisheng, CHEN Fude, HUANG Zinuo, FENG Tianyu, ZHANG Aihui, FANG Baishan. Application of synthetic biology in pesticides residues detection[J]. CIESC Journal, 2021, 72(5): 2413-2425.
毛金竹, 肖淑玲, 杨智淳, 王孝宇, 张诗, 陈俊宏, 谢佶晟, 陈福德, 黄子诺, 冯天宇, 张瑷珲, 方柏山. 合成生物学在农残检测领域的应用[J]. 化工学报, 2021, 72(5): 2413-2425.
化合物 | 关键基因元件 | 底盘生物 | 输出信号 | 检测限(LOD) | 文献 |
---|---|---|---|---|---|
林丹(γ-HCH) | linA2 | E. coli | 电导率 | 2×10-12 | [ |
阿特拉津 | atrABC atzR、atzDEF | E. coli SM004 | 生物发光(LuxCDABE) | 1.08 μmol/L | [ |
对氧磷 | opd | E. coli DH5α | pH | 1 μmol/L | [ |
有机磷 | opd | E. coli | pH | 2 μmol/L | [ |
有机磷 | opd、mpd | E. coli XL1-Blue | 荧光(GFP) | 2 μmol/L | [ |
对氧磷 | opd | S. cerevisiae MT8-1 | 荧光(EGFP) | — | [ |
对氧磷和对硫磷、蝇毒磷 | opd | E. coli | 光纤 | 对氧磷和对硫磷(3 μmol/L) 蝇毒磷(5 μmol/L) | [ |
有机磷 | opd | E. coli BL21 | 吸光度 | 对氧磷(0.2 μmol/L) 对硫磷(0.4 μmol/L) 甲基对硫磷(1 μmol/L) | [ |
有机磷 | opd | Pseudomonas putida JS444 | 电流 | 对氧磷(0.28×10-9) 甲基对硫磷(0.26×10-9) 对硫磷(0.29×10-9) | [ |
有机磷 | opd | Moraxella sp. | 电流 | 对氧磷(0.2 μmol/L) 甲基对硫磷(1 μmol/L) | [ |
毒死蜱 | chpR、chpA-atsBA | E. coli | 生物发光 | 25 nmol/L | [ |
乙基对氧磷 | opd、pnpR-pnpC | E. coli XL1-Blue E. coli DH5α | 颜色 | 1 nmol/L | [ |
3-苯氧基苯甲酸 | VHH、amilCP | E. coli | 沉淀 | 3 ng/ml | [ |
呋喃丹 | H5 DNA | E. coli HB101 | 荧光(EGFP) | 1 ng/L | [ |
Table 1 Application of synthetic biology in pesticide residue detection
化合物 | 关键基因元件 | 底盘生物 | 输出信号 | 检测限(LOD) | 文献 |
---|---|---|---|---|---|
林丹(γ-HCH) | linA2 | E. coli | 电导率 | 2×10-12 | [ |
阿特拉津 | atrABC atzR、atzDEF | E. coli SM004 | 生物发光(LuxCDABE) | 1.08 μmol/L | [ |
对氧磷 | opd | E. coli DH5α | pH | 1 μmol/L | [ |
有机磷 | opd | E. coli | pH | 2 μmol/L | [ |
有机磷 | opd、mpd | E. coli XL1-Blue | 荧光(GFP) | 2 μmol/L | [ |
对氧磷 | opd | S. cerevisiae MT8-1 | 荧光(EGFP) | — | [ |
对氧磷和对硫磷、蝇毒磷 | opd | E. coli | 光纤 | 对氧磷和对硫磷(3 μmol/L) 蝇毒磷(5 μmol/L) | [ |
有机磷 | opd | E. coli BL21 | 吸光度 | 对氧磷(0.2 μmol/L) 对硫磷(0.4 μmol/L) 甲基对硫磷(1 μmol/L) | [ |
有机磷 | opd | Pseudomonas putida JS444 | 电流 | 对氧磷(0.28×10-9) 甲基对硫磷(0.26×10-9) 对硫磷(0.29×10-9) | [ |
有机磷 | opd | Moraxella sp. | 电流 | 对氧磷(0.2 μmol/L) 甲基对硫磷(1 μmol/L) | [ |
毒死蜱 | chpR、chpA-atsBA | E. coli | 生物发光 | 25 nmol/L | [ |
乙基对氧磷 | opd、pnpR-pnpC | E. coli XL1-Blue E. coli DH5α | 颜色 | 1 nmol/L | [ |
3-苯氧基苯甲酸 | VHH、amilCP | E. coli | 沉淀 | 3 ng/ml | [ |
呋喃丹 | H5 DNA | E. coli HB101 | 荧光(EGFP) | 1 ng/L | [ |
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