Performance of fermentative hydrogen production and microbial community in ACR at different influent COD concentrations

doi:10.11949/j.issn.0438-1157.20141345

CIESC Journal ›› 2015, Vol. 66 ›› Issue (3): 1156-1162.DOI: 10.11949/j.issn.0438-1157.20141345

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Performance of fermentative hydrogen production and microbial community in ACR at different influent COD concentrations

CHANG Sheng^1,2, LI Jianzheng², FU Qing¹, ZHAO Xingru¹, ZHENG Guochen²

1 State Environmental Protection Key Laboratory of Drinking Water Source Protection, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
2 School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, Heilongjiang, China

Received:2014-09-03 Revised:2014-11-03 Online:2015-03-05 Published:2015-03-05
Supported by:
supported by the National Natural Science Foundation of China(51178136), the National Major Projects of Water Pollution Control and Treatment (2014ZX07405-001) and the National Environmental Charity Project (201409029).

ACR在不同进水COD浓度下的产氢性能与菌群结构

昌盛^1,2, 李建政², 付青¹, 赵兴茹¹, 郑国臣²

1 中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012;
2 哈尔滨工业大学市政环境工程学院, 黑龙江哈尔滨 150090

通讯作者: 昌盛
基金资助:
国家自然科学基金项目(51178316);国家水体污染控制与治理重大专项(2014ZX07405-001);国家环保公益项目(201409029)。

Abstract

Abstract:

The operation performance of fermentative hydrogen production in anaerobic contact reactor (ACR) at different influent COD concentrations was investigated. The ACR could be kept at steady-state with ethanol-type fermentation, as influent chemical oxygen demand (COD) increased from 7000 mg·L^-1to 11000 mg·L^-1 with constant HRT of 6 h. Specific hydrogen production of the ACR system increased from 2.43 m³·(m³·d)^-1to 3.51 m³·(m³·d)^-1 as influent COD increased from 7000 mg·L^-1 to 11000 mg·L^-1, while specific hydrogen production of activated sludge peaked at 10.71 mol H₂·(kg VSS·d)^-1 at influent COD of 9000 mg·L^-1. The results of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiles showed that hydrogen-producing ethanol fermentative bacteria were dominant in the ACR. As influent COD concentration increased, Ethanoligenens harbinense YUAN-3 became more abundant, and propionate fermentative bacteria, i.e. Propionicimonas sp. F6 also started to be enriched.

Key words: anaerobic contact reactor, fermentative hydrogen production, influent COD concentration, microbial community

摘要：

以稀释糖蜜为底物,通过厌氧接触式发酵制氢反应器(ACR) 的启动和运行,考察了ACR在不同进水COD浓度下的运行特性。结果表明,当HRT= 6 h,进水COD浓度从 7000 mg·L^-1提升至11000 mg·L^-1时,反应器仍能稳定运行,并维持乙醇型发酵类型。随着底物浓度的增加,系统的比产氢速率从COD 7000 mg·L^-1时的2.43 m³·(m³·d)^-1提高到COD11000 mg·L^-1时的3.51 m³·(m³·d)^-1,而活性污泥的比产氢速率在COD 为9000 mg·L^-1时最高,为10.71 mol H₂·(kg VSS·d)^-1。聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)分析结果表明,产氢发酵产乙醇菌群为ACR系统中的主要产氢功能菌群,且随着进水COD浓度的增加,以Ethanoligenens harbinense YUAN-3为代表的产氢菌群的优势度显著增强,但丙酸发酵菌属Propionicimonas sp. F6也开始富集。

关键词: 厌氧接触式反应器, 发酵制氢, 进水COD浓度, 菌群结构

CLC Number:

CHANG Sheng, LI Jianzheng, FU Qing, ZHAO Xingru, ZHENG Guochen. Performance of fermentative hydrogen production and microbial community in ACR at different influent COD concentrations[J]. CIESC Journal, 2015, 66(3): 1156-1162.

昌盛, 李建政, 付青, 赵兴茹, 郑国臣. ACR在不同进水COD浓度下的产氢性能与菌群结构[J]. 化工学报, 2015, 66(3): 1156-1162.

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Performance of fermentative hydrogen production and microbial community in ACR at different influent COD concentrations

ACR在不同进水COD浓度下的产氢性能与菌群结构

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