Abstract:

Filling carriers to anaerobic ammonium oxidation (ANAMMOX) reactor can improve Anammox biomass retention. But there are few researches about the influence of different characteristics of carriers on ANAMMOX biofilm growth. Two different suspending plastic carriers and two different sponge carriers were filled in the reactor to form ANAMMOX biofilm. The results showed that the NH₄⁺ and NO₂^--N average removal rates of one sponge carrier were higher than one suspending plastic carrier on the whole. The EPS contents of sludge on sponge carriers and the speed of sponge carriers forming biofilm were higher than suspending plastic carriers. After biofilm forming for 30 days, the NH₄⁺ and NO₂^--N removal rates of one sponge carrier with low density could be tested and the value of Δ(NO₂^--N)/(NH₄⁺-N) was close to 1.32. After biofilm forming for 105 days, the NH₄⁺ and NO₂^--N average removal rates of one sponge carrier with low density were 0.123 mg·L^-1·h^-1 and 0.160 mg·L^-1·h^-1, respectively. The value of Δ(NO₂^--N)/(NH₄⁺-N) was 1.30, which was close to 1.32. The ANAMMOX activity of sludge on the sponge carrier with low density was the best. The abundance of anammox bacteria in the sludge on the sponge carrier with low density was the highest among the four types of carriers, which was 1.73×10¹⁰ copies·(g dry sludge)^-1. The sponge carrier with low density had the best biofilm formation performance on the whole.

Key words: biofilm, suspending plastic carriers, sponge carriers, anaerobic ammonium oxidation, performance

摘要：

向厌氧氨氧化反应器内投加填料形成生物膜有利于污泥的持留，然而有关填料本身的不同特点对厌氧氨氧化生物膜生长影响的报道较少。将两种不同密度的悬浮塑料填料和两种不同密度的海绵填料置于同一反应器内，进行厌氧氨氧化污泥的挂膜，结果发现海绵填料的单个填料氨氮平均去除速率和亚硝态氮平均去除速率整体高于悬浮塑料填料，所挂污泥的EPS含量整体也高于悬浮塑料填料，并且挂膜速度也相对较快。在挂膜30 d后，单个小密度海绵填料便可检测出氨氮和亚硝态氮去除速率，且Δ（NO₂^--N）/（NH₄⁺-N）值接近1.32。在挂膜105 d后，单个小密度海绵填料的氨氮平均去除速率为0.123 mg·L^-1·h^-1，亚硝态氮平均去除速率为0.160 mg·L^-1·h^-1，值为1.30，最为接近理论值1.32，厌氧氨氧化活性为最佳，并且其所挂污泥的厌氧氨氧化菌丰度值在4种填料中最大，为1.73×10¹⁰ copies·（g dry sludge）^-1，总体来看小密度海绵填料的挂膜效果更好。

关键词: 生物膜, 悬浮塑料填料, 海绵填料, 厌氧氨氧化, 性能