Abstract: Denitrification anaerobic methane oxidation (DAMO) is a recently discovered process in environmental science and engineering field. DAMO process releases a large of free energy, but DAMO microbial growth is extremely slow. The doubling time is as long as one month or more, which makes it difficult to attain DAMO enrichment culture. Methane is thought to be the key element on DAMO bacteria enrichment, because it is insoluble in water. A mathematical model of methane’s behavior in gas-liquid-bacteria phases was set up to study the effect of methane on DAMO bacteria enrichment. According to the model analysis, we obtained the relational expression between DAMO specific activity, methane partial pressure in gas phase and resistance of mass transfer that was confirmed by DAMO specific activity test.

Key words: DAMO, methane, mass transfer model

摘要： 以甲烷为电子供体还原亚硝酸盐的反硝化型甲烷厌氧氧化（Denitrification Anaerobic Methane Oxidation，DAMO）是最近环境科学与工程领域的一个重大发现。虽然DAMO过程释放的自由能非常大，但是DAMO微生物生长却极其缓慢，细胞倍增时间长达一个月以上，DAMO富集培养物难以获得。由于甲烷是一种不易溶于水的气体，因此一般认为甲烷是DAMO微生物富集培养的限制因素，对此建立了甲烷在气-液-菌三相间的传质模型，研究了甲烷溶解度和传质阻力对DAMO微生物富集培养的影响。根据模型分析，得到了DAMO比活性与气相甲烷分压和甲烷传质阻力间的关系式，并通过DAMO活性试验验证了该关系式。

关键词: DAMO, 甲烷, 传质模型