Research progress of aptamer in biochemical separation and detection

doi:10.3969/j.issn.0438-1157.2013.01.025

Abstract

Abstract: Aptamers are short single stranded DNA or RNA oligonucleotides that exhibit high affinity and specificity to their corresponding targets.Compared to other recognition molecules, aptamers are highly stable, easier to synthesize and modify, smaller in size and specific to a wide range of targets.Until now, aptamers have shown diverse applications to detection, separation and purification studies, and development of medical treatment.This paper summarizes the research progress of aptamer in biochemical separation and detection.

Key words: aptamers, recognition-molecules, biochemical separation, detection

摘要： 核酸适配体是一种能够特异性地识别目标物的寡聚核苷酸,可以是RNA也可以是DNA。较其他识别分子而言,适配体具有性质稳定、易合成、易标记、分子量较小和目标分子广泛等优势。目前核酸适配体主要被应用在检测、分离纯化和医疗三大领域,本文主要论述了适配体在生化分离及检测领域中的研究进展。

关键词: 核酸适配体, 识别分子, 生化分离, 检测

CLC Number:

TQ937

LI Xiaopei, YANG Liangrong, HUANG Kun, LI Wensong, SUN Xitong, LIU Huizhou. Research progress of aptamer in biochemical separation and detection[J]. CIESC Journal, 2013, 64(1): 233-242.

李晓佩, 杨良嵘, 黄昆, 李文松, 孙西同, 刘会洲. 核酸适配体在生化分离及检测领域中的研究进展[J]. 化工学报, 2013, 64(1): 233-242.

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