Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO2 whiskers

doi:10.3969/j.issn.0438-1157.2013.06.035

Abstract

Abstract: Mesoporous TiO₂ whiskers (MTiO₂_ws) were chemically modified with 3-aminopropyltriethyloxy silane (APTES).As a result, the point of zero charge (pzc) for the modified material (MTiO₂_ws-APTES) rose from 5.3 to 6.8.Also the specific surface area of MTiO₂_ws-APTES slightly increased, but the pore structure had little change compared to that of MTiO₂_ws.Then, MTiO₂_ws-APTES was used as the support for γ-glutamyltranspeptidase (GGT) immobilization.MTiO₂_ws-APTES showed better loading capacity than MTiO₂_ws.While the enzyme concentration was less than 150 U·g^-1, the recovery of activity was above 99% and the highest specific activity for immobilized GGT (MTiO₂_ws-APTES-GGT) was 184.0 U·g^-1.Although both optimal temperature and thermal stability of MTiO₂_ ws-APTES-GGT slightly decreased compared with the native enzyme, pH stability was greatly improved.The affinity constant (K_m) of MTiO₂_ws-APTES-GGT toward γ-glutamyl-p-nitroaniline (GpNA) was also determined to be 0.889 mmol·L^-1, which was higher than that of the native enzyme, but less than that of MTiO₂_ws-GGT.After storage at 4℃ for 60 days and reused for 21 batches, MTiO₂_ws-APTES-GGT maintained 80.07% of its initial activity.

Key words: mesoporous TiO₂ whiskers, chemical modification, 3-aminopropyltriethyloxy silane, enzyme immobilization, stability, loading capacity

摘要： 以3-氨丙基三乙氧基硅烷(APTES)对介孔二氧化钛晶须(MTiO₂_ws)进行表面修饰,使其电荷零点值(pzc)由5.3提高至6.8,改性后材料MTiO₂_ws-APTES的比表面积略有上升,但孔结构基本不变。以MTiO₂_ws-APTES为载体对γ-谷氨酰转肽酶(GGT)进行了固定化,当给酶量小于150 U·g^-1时,酶活回收率均大于99%,固定化酶MTiO₂_ws-APTES-GGT的比活力最高可达184.0 U·g^-1,对酶的负载性能显著优于MTiO₂_ws。相比于游离酶,MTiO₂_ws-APTES-GGT的最适温度和热稳定性均略有下降,但pH稳定性明显优于游离酶和以MTiO₂_ws为载体的固定化酶(MTiO₂_ws-GGT); MTiO₂_ws-APTES-GGT对γ-谷氨酰对硝基苯胺(GpNA)的亲和力常数(K_m)为0.889 mmol·L^-1,较游离酶有所上升,但小于MTiO₂_ws-GGT。MTiO₂_ws-APTES-GGT的稳定性良好,经4℃下储藏60 d,连续使用21批次后残余酶活仍可达初始值的80.07%。

关键词: 介孔氧化钛晶须, 化学改性, 3-氨丙基三乙氧基硅烷, 固定化酶, 稳定性, 担载量

CLC Number:

Q814

YE Lijing, FANG Xin, WANG Haoqi, YAO Zhong, XIONG Qiang, ZHOU Zhi, SUN Yun, WEI Ping. Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO₂ whiskers[J]. CIESC Journal, 2013, 64(6): 2160-2168.

叶丽静, 房鑫, 王浩绮, 姚忠, 熊强, 周治, 孙芸, 韦萍. 介孔TiO₂晶须材料的硅烷化改性及其对载体pzc值和酶负载性能的影响[J]. 化工学报, 2013, 64(6): 2160-2168.

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Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO₂ whiskers

介孔TiO₂晶须材料的硅烷化改性及其对载体pzc值和酶负载性能的影响

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