Coupling process and mechanism of methanol oxidation and nitrate reduction in an anodic denitrification microbial fuel cell(AD-MFC)

doi:10.3969/j.issn.0438-1157.2013.09.044

CIESC Journal ›› 2013, Vol. 64 ›› Issue (9): 3404-3411.DOI: 10.3969/j.issn.0438-1157.2013.09.044

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Coupling process and mechanism of methanol oxidation and nitrate reduction in an anodic denitrification microbial fuel cell(AD-MFC)

ZHANG Jiqiang¹, ZHENG Ping¹, ZHANG Meng¹, LI Wei¹, CHEN Hui¹, CAI Chen¹, XIE Zuofu^1,2

1. Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, Zhejiang, China;
2. Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China

Received:2013-01-06 Revised:2013-02-28 Online:2013-09-05 Published:2013-09-05
Supported by:
supported by the National Natural Science Foundation of China(31070110),the Specialized Research Fund for the Doctoral Program of Higher Education of China(20110101110078),the Natural Science Foundation of Zhejiang Province(Z5110094,Y5110266).

AD-MFC中甲醇与硝酸盐的偶合过程与作用机制

张吉强¹, 郑平¹, 张萌¹, 厉巍¹, 陈慧¹, 蔡琛¹, 谢作甫^1,2

1. 浙江大学环境工程系, 浙江杭州 310058;
2. 浙江科技学院, 浙江杭州 310023

通讯作者: 郑平
作者简介:张吉强(1986- ),男,博士研究生。
基金资助:
国家自然科学基金项目(31070110);高等学校博士学科点专项科研基金(20110101110078);浙江省自然科学基金重点项目(Z5110094);浙江省自然科学基金项目(Y5110266)。

Abstract

Abstract: An anodic denitrification MFC(AD-MFC)was constructed by inoculating denitrifying bacteria enrichment culture into anode chamber of a dual-chamber MFC,and its capacity of denitrification and electricity production was investigated with methanol as electron donor and nitrate as acceptor in a batch cultivation mode.When initial nitrate and COD concentration were (100.22±0.62)mg·L^-1 and (500.40±1.67)mg·L^-1,the volumetric nitrogen and COD removal rates reached 0.31 kg N·m^-3·d^-1 and 1.06 kg COD·m^-3·d^-1,respectively,coupled with the maximum voltage of (602.80±5.42)mV and the maximum power density of (908.42±0.07)mW·m^-3.The results demonstrated that power generation in the AD-MFC was due to a coupling of methanol oxidation and nitrate reduction,and voltage changes were closely related to denitrification,indicating the course of denitrification.Voltage curves of the AD-MFC showed three-phase characteristics of descend phase,ascend phase and redescend phase,which could be ascribed to the succession of denitrification,methanol degradation and cell hydrolysate fermentation as dominant reaction in the anolyte.

Key words: microbial fuel cell, biological denitrification, electricity generation, nitrogen removal

摘要： 在双室微生物燃料电池(MFC)阳极内接种反硝化细菌富集培养物,同时加入硝酸盐和甲醇,构建了阳极反硝化微生物燃料电池(AD-MFC),并以批式操作研究了AD-MFC的反硝化产电性能。试验结果表明,在初始硝氮浓度为(100.22±0.62)mg·L^-1,COD浓度为(500.40±1.67)mg·L^-1的条件下,AD-MFC的最大容积NO₃^--N和COD去除速率分别达到0.31 kg N·m^-3·d^-1和1.06 kg COD·m^-3·d^-1,最大电压达到(602.80±5.42)mV,相应最大功率密度为(908.42±0.07)mW·m^-3。AD-MFC的产电过程是甲醇氧化与硝酸盐还原的偶合过程,电压变化与反硝化作用密切相关,可用于指示反硝化进程。AD-MFC的电压曲线呈现降低-升高-再降低的三阶段特性,其原因是反硝化作用、甲醇降解作用和细胞水解发酵作用依次成为阳极液中的主导反应。

关键词: 微生物燃料电池, 反硝化作用, 产电性能, 脱氮性能

CLC Number:

X703.1

ZHANG Jiqiang, ZHENG Ping, ZHANG Meng, LI Wei, CHEN Hui, CAI Chen, XIE Zuofu. Coupling process and mechanism of methanol oxidation and nitrate reduction in an anodic denitrification microbial fuel cell(AD-MFC)[J]. CIESC Journal, 2013, 64(9): 3404-3411.

张吉强, 郑平, 张萌, 厉巍, 陈慧, 蔡琛, 谢作甫. AD-MFC中甲醇与硝酸盐的偶合过程与作用机制[J]. 化工学报, 2013, 64(9): 3404-3411.

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Coupling process and mechanism of methanol oxidation and nitrate reduction in an anodic denitrification microbial fuel cell(AD-MFC)

AD-MFC中甲醇与硝酸盐的偶合过程与作用机制

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