Effect of temperature on methanogens metabolic pathway and structures of predominant bacteria

doi:10.3969/j.issn.0438-1157.2014.05.007

Effect of temperature on methanogens metabolic pathway and structures of predominant bacteria

WU Meirong^1,2, ZHANG Rui^1,2, ZHOU Jun^1,2, XIE Xinxin^1,2, YONG Xiaoyu^1,2, YAN Zhiying³, GE Mingmin^1,2, ZHENG Tao^1,2

1 School of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China;
2 Bioenergy Research Institute, Nanjing Tech University, Nanjing 211816, Jiangsu, China;
3 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China

Received:2014-01-02 Revised:2014-02-09 Online:2014-05-05 Published:2014-05-05
Supported by:
supported by the National Basic Research Program of China (2013CB733504), the National Natural Science Foundation of China (21307058, 21207065), the Jiangsu Agricultural Independent Innovation Project (CX(13)3045) and the Chinese Academy of Environmental and Application of Microbial Key Laboratory Fund Project (KLCAS-2013-05).

温度对产甲烷菌代谢途径和优势菌群结构的影响

吴美容^1,2, 张瑞^1,2, 周俊^1,2, 谢欣欣^1,2, 雍晓雨^1,2, 闫志英³, 葛明民^1,2, 郑涛^1,2

1 南京工业大学生物与制药工程学院, 江苏南京 211816;
2 南京工业大学生物能源研究所, 江苏南京 211816;
3 中国科学院成都生物研究所, 四川成都 610041

通讯作者: 郑涛
基金资助:
国家重点基础研究发展计划项目（2013CB733504）；国家自然科学基金项目（21307058，21207065）；江苏农业自主创新项目（CX（13）3045）；中国科学院环境与应用微生物重点实验室开放基金项目（KLCAS-2013-05）。

Abstract

Abstract: Methanogens are strictly anaerobic archaea, which not only take part in the methanogenesis procedure but also limit this process. Temperature plays a key role in the anaerobic fermentation. Temperature could not only directly alter the community structure and function of methanogenic archaea,but also affect the supply of substrates for methanogens,which in turn indirectly regulates the pathways of methanogenic archaea．There are three pathway for methanogenesis, and they are started from acetic acid, H₂/CO₂ and C-1 compound respetively. Acetoclastic methanogenesis accounts for about two-thirds of the total methane production globally, while hydrogenotrophic methanogenesis accounts for about one third. Methanol- and methyl amine-derived methanogensis is restricted in ocean and saline water. Acetoclastic methanogenesis is the predominant methanogenesis at a low temperature, and methane is produced by acetoclastic and hydrogenotrophic methanogenesis at a medium temperature, while methane is exclusively produced by hydrogenotrophic methanogenesis at a high or ultra-high temperature.

Key words: methane, bioenergy, metabolism, methanogens, temperature, acetoclastic methanogenesis, hydrogenotrophic methanogenesis

摘要： 产甲烷菌是严格厌氧的古菌，由其完成的产甲烷过程通常是厌氧微生物生化代谢中最重要的限速步骤。温度作为影响产甲烷菌的产甲烷速率重要因素，其变化会改变生物环境中的产甲烷的代谢途径和优势菌群分布。目前已知甲烷生物合成有3条途径：乙酸代谢途径、CO₂还原途径和甲基营养型途径。理论上乙酸途径生成的甲烷约占甲烷生成总量的2/3，CO₂还原产甲烷途径则约占1/3，甲基营养型途径只在少数情况下考虑其影响，例如盐湖。在低温条件下产甲烷菌以利用乙酸代谢为主；在中温条件下，产甲烷途径以乙酸代谢和H₂/CO₂还原一定比例存在；在高温和超高温条件下，以只利用CO₂还原途径的菌群为主。

关键词: 甲烷, 生物能源, 代谢, 产甲烷菌, 温度, 乙酸代谢途径, CO₂还原产甲烷途径

CLC Number:

Q939.9

WU Meirong, ZHANG Rui, ZHOU Jun, XIE Xinxin, YONG Xiaoyu, YAN Zhiying, GE Mingmin, ZHENG Tao. Effect of temperature on methanogens metabolic pathway and structures of predominant bacteria[J]. CIESC Journal, DOI: 10.3969/j.issn.0438-1157.2014.05.007.

吴美容, 张瑞, 周俊, 谢欣欣, 雍晓雨, 闫志英, 葛明民, 郑涛. 温度对产甲烷菌代谢途径和优势菌群结构的影响[J]. 化工学报, DOI: 10.3969/j.issn.0438-1157.2014.05.007.

References 30

[1]	Liu Chang(刘畅), Lu Xiaohua(陆小华). Carbon reduction pattern in China: comparison of CCS and biomethane route [J]. CIESC Journal (化工学报), 2012, 61(1):7-10
[2]	Gong Weijia(公维佳), Li Wenzhe(李文哲), Liu Jianyu(刘建禹). Progress of research on methanogens bacteria inanaerobic digestion [J]. Journal of Northeast Agricultural University(东北农业大学学报), 2006, 37(6): 838-841
[3]	Shan Liwei(单丽伟), Feng Guiying(冯贵颖), Fan Sanhong(范三红). Process in Genome and Methanogenesis of Methanogens[J]. Journal of Microbiology(微生物学杂志), 2003, 23(6): 42-46
[4]	Oremland R S, Marsh L, Des Marais D J. Methanogenesis in Big Soda Lake, Nevada: an alkaline, moderately hypersaline desert lake[J]. Appl. Environ. Microb., 1982,43: 462-468
[5]	Conrad R. Contribution of hydrogen to methane production and control of hydrogen concentrations in methanogenic soils and sediments [J]. FEMS Microbiology Ecology, 1999, 28(3): 193-202
[6]	Karakashev D, Batstone D J, Trably E, et al. Acetate oxidation is the dominant methanogenic pathway from acetate in the absence of methanosaetaceae [J]. Applied and Environmental Microbiology, 2006, 72(7): 5138-5141
[7]	Conrad R. Quantification of methanogenic pathways using stable carbon isotopic signatures: a review and a proposal [J]. Organic Geochemistry, 2005, 36(5): 739-752
[8]	Avery G B, Shannon R D, White J R, et al．Controls on methane production in a tidal freshwater estuary and a peatland: methane production via acetate fermentation and CO2 reduction [J]. Biogeochemistry, 2003, 62:19-37
[9]	Zuo Jiane, Xing Wei. Department of environmental science and engineering[J]. Chin. J. Appl. Ecol., 2007, 18(9): 2127- 2132
[10]	Akila G, Chandra T S. Performance of an UASB reactor treating synthetic wastewater at low temperature using cold adapted seed slurry [J]. Process Biochemisty, 2007, 42: 466-471
[11]	Jiang Na(蒋娜), Chen Zijuan(陈紫娟), Dong Xiuzhu(东秀珠). Methanogenic archaea and their mediated methanogenic pathways in cold wetland [J]. Microbiol. China(微生物学通报), 2013, 40(1): 146-157
[12]	Chin K J, Conrad R. Intermediary metabolism in methanogenic paddy soil and the influence of temperature[J]. FEMS Microbiology Ecology, 1995, 18(2): 85-102
[13]	Kotsyurbenko O R, Nozhevnikova A N, Zavarzin G A. Methanogenic degradation of organic matter by anaerobic bacteria at low temperature[J]. Chemosphere, 1993, 27(9): 1745-1761
[14]	Kotsyurbenko O R, Nozhevnikova A N, Soloviova T I, et al. Methanogenesis at low temperatures by microflora of tundra wetland soil [J]. Antonie van Leeuwenhoek, 1996, 69(1): 75-86
[15]	Zhang D D, Zhu W B, Tang C, Suo Y L, Gao L J, Yuan X F, Wang X F, Cui Z J. Bioreactor performance and methanogenic population dynamics in a low-temperature(5—18℃)anaerobic fixed-bed reactor[J]. Bioresource Technology, 2012, 104: 136-143
[16]	Großkopf R, Janssen P H, Liesack W. Diversity and structure of the methanogenic community in anoxic rice paddy soil microcosms as examined by cultivation and direct 16S rRNA gene sequence retrieval[J]. Applied and Environmental Microbiology, 1998, 64(3): 960-969
[17]	Conrad R. Contribution of hydrogen to methane production and control of hydrogen concentrations in methanogenic soils and sediments [J]. FEMS Microbiology Ecology, 2006, 28: 193-202
[18]	Calli B, Mertoglu B, Roest K, et al. Comparison of long-term performances and final microbial compositions of anaerobic reactors treating landfill leachate [J]. Bioresource Technology, 2006, 97(4): 641-647
[19]	Fey A, Claus P, Conrad R. Temporal change of 13C-isotope signatures and methanogenic pathways in rice field soil incubated anoxically at different temperatures [J]. Geochimica et Cosmochimica Acta, 2004, 68(2): 293-306
[20]	Conrad R, Klose M, Noll M. Functional and structural response of the methanogenic microbial community in rice field soil to temperature change[J]. Environ. Microbiol., 2009, 11: 1844-1853
[21]	Wu X L, Friedrich M W, Conrad R. Diversity and ubiquity of thermophilic methanogenic archaea in temperate anoxic soils [J]. Environ. Microbiol., 2006, 8: 394-404
[22]	Qu Xian(瞿贤), He Pinjing(何品晶). Effect of temperature on methanogenic pathway during household waste anaerobic digestion by stable carbon isotopic signature of CH4 [J]. Environmental Science(环境科学), 2008, 29(11) : 3252-3257
[23]	Lu Y, Conrad R. In situ stable isotope probing of methanogenic archaea in the rice rhizosphere [J]. Science, 2005, 309: 1088-1090
[24]	Ahring B K, rahim A A, adenovska Z. Effect of temperature increase from 55℃ to 65℃ on performance and microbial population dynamics of an anaerobic reactor treating cattle manure [J]．Water Research, 2001, 35: 2446-2452
[25]	Wang Changwen(王长文), Pu Guibing(蒲贵兵), Lü Bo(吕波), He Dong(何东), Yin Hongjun(尹洪军). Study on approaches to reduce hydrogen partial pressure during anaerobic digestion of sewage sludge [J]. Chemistry & Bioengineering(化学与生物工程), 2010, 27(4): 79-82
[26]	Burggraf S, Fricke H, Neuner A, Kristjansson J, Rouvier P, Mandelco L, Woese C R, Stetter K O. Methanococcus igneus sp. nov.,a novel hyperthermophilic methanogen from a shallow submarine hydrothermal system[J]. Syst. Appl. Microbiol., 1990, 13: 263-269
[27]	Fiala G, Stetter K O. Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100℃[J]. Arch. Microbiol, 1986, 145: 56-61
[28]	Takai K, et al. Cell proliferation at 122℃ and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation[J]. Proc. Natl. Acad. Sci. USA, 2008, 105: 10949-10954
[29]	Liu Haichang(刘海昌), Lan Guihong(兰贵红). Isolation and identification of a methanogen from the high temperature oil reservoir water [J]. China J. Biotech.(生物工程学报), 2010, 26(7): 1009-1013
[30]	Yang Wei(杨薇). Industry prospect and specific properties of methanogens [J]. Anhui Chemical Industry(安徽化工), 2010(4): 10-13