KINETICS OF ENZYME-CATALYZED REACTIONS IN BATCH REACTOR USING THERMOKINETIC METHOD

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KINETICS OF ENZYME-CATALYZED REACTIONS IN BATCH REACTOR USING THERMOKINETIC METHOD

Liang Yi,Wang Cunxin and Qu Songsheng( Department of Chemistry , WuhanUniversity, Wuhan 430072)Wu Yuanxin and Li Dinghuo ( Department of Chemical Engineering , Wuhan Institute of Chemical Technology, Wuhan 430074)

Online:1997-02-25 Published:1997-02-25

热动力学法研究间歇反应器中酶促反应动力学

梁毅,汪存信,屈松生,吴元欣,李定或

武汉大学化学系,武汉大学化学系,武汉大学化学系,武汉化工学院化工系,武汉化工学院化工系武汉 430072 ,武汉 430072 ,武汉 430072 ,武汉 430074 ,武汉 430074

Abstract

Abstract: Kinetics of single-substrate enzyme-catalyzed reactions in batch reactor (BR) using thermokinetic method were investigated , and the influence of product competitive inhibition on these models was discussed in details. Catalase-catalyzed decomposition of H2O2 and arginase-catalyzed hydrolysis of L-arginine were studied by microcalorimetry, and the reliability of this thermokinetic method applied to the study of kinetics of single-substrate enzyme-catalyzed reactions in BR has been verified by the experimental data.

摘要： 用热动力学法研究了间歇反应器中单底物酶促反应动力学,并详细讨论了产物竞争性抑制作用对反应速率的影响.用微量热法研究了过氧化氢酶催化分解过氧化氢和精氨酸酶催化水解L-精氨酸的反应,实验验证了本文热动力学法研究间歇反应器中单底物酶促反应动力学的正确性.

CLC Number:

TQ033

梁毅,汪存信,屈松生,吴元欣,李定或. 热动力学法研究间歇反应器中酶促反应动力学 [J]. CIESC Journal.

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