CIESC Journal ›› 2018, Vol. 69 ›› Issue (1): 259-271.DOI: 10.11949/j.issn.0438-1157.20170964

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Advances in regeneration system of natural nicotinamide cofactor and its artificial analogues

ZHU Chenjie, FU Jingwen, TAN Zhuotao, YING Hanjie   

  1. National Engineering Technique Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China
  • Received:2017-07-25 Revised:2017-09-11 Online:2018-01-05 Published:2018-01-05
  • Contact: 10.11949/j.issn.0438-1157.20170964
  • Supported by:

    supported by the National Natural Science Foundation of China (21776132, 21406110, 21390204), Jiangsu Province Natural Science Foundation for Youths (BK20140938) and Young Elite Scientist Sponsorship Program by CAST.

天然烟酰胺辅因子再生体系及其人工类似物研究进展

朱晨杰, 付静雯, 谭卓涛, 应汉杰   

  1. 南京工业大学生物与制药工程学院, 国家生化工程技术研究中心, 江苏 南京 211816
  • 通讯作者: 应汉杰
  • 基金资助:

    国家自然科学基金项目(21776132,21406110,21390204);江苏省自然科学基金项目(BK20140938);“青年人才托举工程”项目。

Abstract:

Oxidoreductases have drawn considerable attention as mild and efficient catalysts in the fields of organic synthesis and medical sciences due to they can catalyze regio-, chemo-and stereoselective transformations that cannot be easily achieved by chemical catalysts. Cofactors are required in oxidoreductase-catalyzed reactions, commonly in the form of nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate, abbreviated as NAD(H) and NADP(H). Given the high cost and physical instability of NAD(H)/NADP(H), the stoichiometric usage is not practical for industrial applications. After decades, four main strategies such as enzymatic, chemical, electrochemical and photochemical method have been used for the cofactor regeneration. Meanwhile, the development of stable, highly active and inexpensive artificial nicotinamide cofactors particularly 1,4-dihydropyridine derivatives has led to a new breakthrough.

Key words: biocatalysis, enzyme, nicotinamide cofactor, electrochemistry, photochemistry, artificial cofactor

摘要:

氧化还原酶可以催化具有特定区域选择性、化学选择性、立体选择性的反应,反应条件温和且催化效率高,因此在有机化学和制药领域发挥着日益重要的作用。绝大多数氧化还原酶依赖烟酰胺腺嘌呤二核苷酸NAD(H)和烟酰胺腺嘌呤二核苷酸磷酸NADP(H)为酶促反应提供氧化还原当量。NAD(H)/NADP(H)由于价格昂贵、稳定性差导致无法化学计量投入。经过几十年研究,形成了4种经典的NAD(H)/NADP(H)再生方法:酶法、化学法、电化学法和光化学法,与此同时,一系列稳定性好、活性高且廉价的人工烟酰胺辅因子mNAD(H) s尤其是1,4-二氢吡啶类烟酰胺辅因子的开发和利用为NAD(H)/NADP(H)工业化运用提供了新的思路。

关键词: 生物催化, 酶, 烟酰胺辅因子, 电化学, 光化学, 人工辅因子

CLC Number: