Adsorption of Protein in the Expanded Bed

Abstract

Abstract: The influence of operation parameters on the adsorption performance of protein (bovin serum
albumin, BSA) in an expanded bed was studied using Streamline diethyl aminoethyl (DEAE).
The result of residence time distributions (RTD) and breakthrough curves showed that
adsorption performance of the expanded bed could not be improved by increasing the flow
velocity at the range from 16 ml·min-1 to 26 ml.min-1. The increase of protein
concentration from 0.5 mg·m1-1 to 2 mg.m1-1 resulted in poor adsorption performance. With
increasing temperature from 5℃ to 30℃ and the sedimented bed height from 11.5cm to
22.5cm, the adsorption characteristics in the expanded bed were improved.

Key words: adsorption performance, expanded bed, residence time distribution, breakthrough curve, protein

摘要： The influence of operation parameters on the adsorption performance of protein (bovin serum
albumin, BSA) in an expanded bed was studied using Streamline diethyl aminoethyl (DEAE).
The result of residence time distributions (RTD) and breakthrough curves showed that
adsorption performance of the expanded bed could not be improved by increasing the flow
velocity at the range from 16 ml·min-1 to 26 ml.min-1. The increase of protein
concentration from 0.5 mg·m1-1 to 2 mg.m1-1 resulted in poor adsorption performance. With
increasing temperature from 5℃ to 30℃ and the sedimented bed height from 11.5cm to
22.5cm, the adsorption characteristics in the expanded bed were improved.

关键词: adsorption performance;expanded bed;residence time distribution;breakthrough curve; protein

HU Hongbo, YAO Shanjing, LIN Dongqiang, ZHU Ziqiang. Adsorption of Protein in the Expanded Bed[J]. .

胡洪波, 姚善泾, 林东强, 朱自强. 肿胀床中蛋白质吸附性能[J]. CIESC Journal.

References

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