Simultaneous separation of glycyrrhizic acid and baicalin by mixing Glycyrrhiza with Scutellaria using foam fractionation

doi:10.11949/j.issn.0438-1157.20160920

Abstract

Abstract:

For simultaneous separating glycyrrhizic acid with surface activity from Glycyrrhiza and baicalin without surface activity from Scutellaria simultaneously, a novel technology for foam fractionation of the leaching liquor of Glycyrrhiza and Scutellaria was developed.The fluorescence emission spectra, UV absorption spectra and FTIR spectra showed that there was complexing action between glycyrrhizic acid and baicalin.The compatibility of Glycyrrhiza and Scutellaria enhanced the extraction of glycyrrhizic acid and baicalin, accomplished the separation of baicalin by foam fractionation, and improved the foam fractionation performances of glycyrrhizic acid.Using enrichment ratio and recovery percentage as the evaluation indexes, the effects of temperature, volumetric air flow rate, the initial concentration of glycyrrhizic acid and the mass ratio of Glycyrrhiza and Scutellaria on the foam fractionation performances were investigated.The results showed that the enrichment ratio and the recovery percentage of glycyrrhizic acid reached 11.0 and 73.5% with the enrichment ratio and the recovery percentage of baicalin reached 5.8 and 38.5%, respectively, under the conditions of temperature 40℃, volumetric air flow rate 100 ml·min^-1, the initial concentration of glycyrrhizic acid 0.2 g·L^-1 and the mass ratio of Glycyrrhiza and Scutellaria 3:1.In this work, baicalin was separated by foam fractionation for the first time.Compared to the technology of foam fractionation of the leaching liquor from Glycyrrhiza, the novel one increased the enrichment ratio and the recovery percentage by 194.9% and 23.3%, respectively.Therefore, the addition of Scutellaria effectively improved the foam fractionation performances of glycyrrhizic acid and baicalin.

Key words: foam, separation, leaching, glycyrrhizic acid, baicalin

摘要：

为了同时分离甘草中有表面活性的甘草酸和黄芩中无表面活性的黄芩苷，开发了甘草配伍黄芩泡沫分离工艺。通过荧光光谱、紫外吸收光谱和红外吸收光谱分析表明，甘草酸与黄芩苷存在相互作用，并且甘草黄芩配伍强化了甘草酸和黄芩苷的提取。以甘草酸和黄芩苷的富集比和回收率为评价指标，当温度为40℃、气体体积流量为100 ml·min^-1、甘草酸初始浓度为0.2 g·L^-1、甘草黄芩质量比为3：1时，获得甘草酸的富集比和回收率分别为11.0和73.5%，黄芩苷的富集比和回收率分别为5.8和38.5%。通过甘草与黄芩配伍，利用泡沫分离获得黄芩中的黄芩苷。同时，与单独泡沫分离甘草中甘草酸相比，甘草酸的富集比提高了194.9%，回收率提高了23.3%。因此，甘草配伍黄芩能有效泡沫分离甘草酸和黄芩苷。

关键词: 泡沫, 分离, 浸取, 甘草酸, 黄芩苷

CLC Number:

DING Linlin, WANG Yanji, WU Zhaoliang, DENG Fei, LI Rui, LIU Wei. Simultaneous separation of glycyrrhizic acid and baicalin by mixing Glycyrrhiza with Scutellaria using foam fractionation[J]. CIESC Journal, 2017, 68(1): 170-177.

丁琳琳, 王延吉, 吴兆亮, 邓菲, 李瑞, 刘伟. 基于甘草配伍黄芩同时泡沫分离甘草酸和黄芩苷[J]. 化工学报, 2017, 68(1): 170-177.

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