Abstract:

The denitrifying glycan bacteria were successfully enriched in the SBR reactor with sodium acetate as the carbon source and \mathrm{N}{\mathrm{O}}_3^{-}-N as the electron acceptor, and the effects of the influent C/N ratio (3.3,6.7,10), electron acceptors (\mathrm{N}{\mathrm{O}}_3^{-}-N, \mathrm{N}{\mathrm{O}}_2^{-}-N), carbon source types (sodium acetate, glucose) on the activity of denitrifying glycans and the conversion characteristics of internal carbon sources were further investigated by batch experiment. The results show that the higher the influent C/N ratio, the higher the \mathrm{N}{\mathrm{O}}_x^{-}-N removal rate of the system, and the more PHB synthesis in the anaerobic section, but the influent C/N ratio is too high, which leads to the predominance of common denitrifying bacteria. The denitrification efficiency of the internal carbon source is suitable for the influent C/N ratio of 6.7. The DGAOs system with \mathrm{N}{\mathrm{O}}_3^{-}-N as the electron acceptor for long-term cultivation is not domesticated by \mathrm{N}{\mathrm{O}}_2^{-}-N, and has good anti-\mathrm{N}{\mathrm{O}}_2^{-}-N. Nitrification performance, after adding the same concentration of \mathrm{N}{\mathrm{O}}_2^{-}-N as \mathrm{N}{\mathrm{O}}_3^{-}-N, the system NO_x^--N removal rate is 89.6%; when glucose is used as the carbon source, the amount of PHB synthesized by DPAOs in the anaerobic section is only 79.5% of PHB with sodium acetate as the carbon source, and the anaerobic glucose utilization rate is only 72.8%, which is much smaller than the utilization rate of sodium acetate.

Key words: denitrifying glycan, internal carbon source denitrification, polymers, anaerobic, absorption

摘要：

在SBR反应器中以乙酸钠为碳源、\mathrm{N}{\mathrm{O}}_3^{-}-N为电子受体成功富集了反硝化聚糖菌，并采用批次实验进一步考察了进水C/N比（3.3,6.7,10）、电子受体（\mathrm{N}{\mathrm{O}}_3^{-}-N、\mathrm{N}{\mathrm{O}}_2^{-}-N）、碳源类型（乙酸钠、葡萄糖）对反硝化聚糖菌活性的影响及内碳源转化特性。实验结果表明，进水C/N比越高，系统\mathrm{N}{\mathrm{O}}_x^{-}-N去除率越高，厌氧段合成PHB越多，但进水C/N比过高会导致普通反硝化菌占优势，影响内碳源反硝化效率，进水C/N比为6.7较为合适；以\mathrm{N}{\mathrm{O}}_3^{-}-N为电子受体长期培养的DGAOs系统未经\mathrm{N}{\mathrm{O}}_2^{-}-N驯化，对\mathrm{N}{\mathrm{O}}_2^{-}-N同样具有良好的反硝化性能，在投加与\mathrm{N}{\mathrm{O}}_3^{-}-N相同浓度的\mathrm{N}{\mathrm{O}}_2^{-}-N后，系统\mathrm{N}{\mathrm{O}}_x^{-}-N去除率达89.6%；当以葡萄糖为碳源时，DPAOs在厌氧段合成的PHB的量仅为以乙酸钠为碳源时合成PHB量的79.5%，且厌氧段葡萄糖利用率仅为72.8%，远远小于乙酸钠的利用率。

关键词: 反硝化聚糖菌, 内碳源反硝化, 聚合物, 厌氧, 吸收