Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate

Abstract

Key words: 2, 3-butanediol production, optimization, fed-batch, Klebsiella pneumoniae

摘要： The production of 2,3-butanediol by Klebsiella pneumoniae from glucose supplemented with different salts was studied. A suitable medium composition was defined by response surface experiments. In a medium containing glu-cose and (NH4)2HPO4, the strain could convert 137.0g of glucose into 52.4g of 2,3-butanediol and 8.4g of acetoin in shaking flasks. The diol yield amounted to 90% of its theoretical value and the productivity was 1—1.5g?L－1?h－1. In fed-batch fermentation, the yield and productivity of diol were further enhanced by maintaining the pH at 6.0. Up to 92.4g of 2,3-butanediol and 13.1g of acetoin per liter were obtained from 215.0g of glucose per liter. The diol yield reached 98% of its theoretical value and the productivity was up to 2.1g?L－1?h－1.

关键词: 2;3-butanediol production;optimization;fed-batch;Klebsiella pneumoniae

QINJiayang(秦加阳),XIAOZijun(肖梓军),MACuiqing(马翠卿),XIENengzhong(谢能中),LIUPeihai(刘培海)andXUPing(许平). Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate[J]. .

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