微酸环境响应的聚天冬氨酸修饰脂质体的制备与表征

doi:10.11949/j.issn.0438-1157.20141374

化工学报 ›› 2015, Vol. 66 ›› Issue (3): 1234-1239.DOI: 10.11949/j.issn.0438-1157.20141374

• 材料化学工程与纳米技术 • 上一篇

微酸环境响应的聚天冬氨酸修饰脂质体的制备与表征

王丽琳, 沈骧一, 苏海佳, 曹辉

北京化工大学北京市生物加工过程重点实验室, 北京 100029

收稿日期:2014-09-15 修回日期:2014-11-28 出版日期:2015-03-05 发布日期:2015-03-05
通讯作者: 苏海佳
基金资助:
国家重点基础研究发展计划项目(2014CB745100);国家高技术研究发展计划项目(2012AA021402);教育部留学回国人员科研启动基金资助项目(LXJJ2012-001)。

Preparation and characterization of subacid environment responsive polyaspartic acid modified liposomes

WANG Lilin, SHEN Xiangyi, SU Haijia, CAO Hui

Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China

Received:2014-09-15 Revised:2014-11-28 Online:2015-03-05 Published:2015-03-05
Supported by:
supported by the National Basic Research Program of China (2014CB745100), the National High Technology Research and Development Program of China (2012AA021402) and the Project-sponsored by SRF for ROCS, SEM (LXJJ2012-001).

摘要/Abstract

摘要：

利用带负电的聚天冬氨酸分子与部分带正电的磷脂双分子层之间的静电吸附作用,通过"一步法"成功制备了聚天冬氨酸(PASP)修饰脂质体(PLPs),实现了修饰脂质体微酸环境响应性。将脂质体制备与PASP修饰同时完成的"一步法"大大简化了制备工艺,提高了脂质体(PLPs)的制备效率。通过单因素筛选确定了pH敏感性显著的修饰脂质体制备条件,即外水相溶液PASP浓度2.5%(质量分数),pH8.5。透射电子显微镜照片显示PLPs由于修饰剂的存在具有更大的粒径,且表面电负性高,证明了"一步法"成功制备了pH敏感性修饰脂质体。

关键词: pH敏感, 聚天冬氨酸, 脂质体, 制备, 纳米粒子

Abstract:

PASP modified liposomes (PLPs) were prepared by using negatively charged PASP chains linked to the head of positively charged phospholipid via electrostatic adsorption. A "one-step" method was developed to form and modify the liposomes. Single factor experiments were conducted to prepare high pH sensitive PLPs. The optimal formula in preparing PLPs was 2.5%(mass) PASP solution with pH8.5. TEM results showed that PASP modified liposomes had a larger particle size and their surface with higher electro-negativity. PASP modified liposomes are a kind of relatively stable emulsion system.

Key words: pH sensitivity, polyaspartic acid, liposomes, preparation, nanoparticles

中图分类号:

R944.1

王丽琳, 沈骧一, 苏海佳, 曹辉. 微酸环境响应的聚天冬氨酸修饰脂质体的制备与表征[J]. 化工学报, 2015, 66(3): 1234-1239.

WANG Lilin, SHEN Xiangyi, SU Haijia, CAO Hui. Preparation and characterization of subacid environment responsive polyaspartic acid modified liposomes[J]. CIESC Journal, 2015, 66(3): 1234-1239.

参考文献 26

[1]	Chen Fei(陈菲), Wang Yumei(王瑜梅). Effect of hydroxycamptothecin on the proliferation and apoptosis of human breast cancer cell line MCF-7 [J]. China Medical Herald(中国医药导报), 2011, 8(10): 17-19
[2]	Feng Xia(冯霞), Liang Shile(梁世乐), Li Xiaofeng(李晓峰), et al. Preparation and antitumor effect of drug delivery system of taxol conjugated to polyethylene glycol [J]. Journal of Chemical Industry and Engineering(China)(化工学报), 2003, 54(2): 209-214
[3]	Danhier F, Feron O, Préat V. To exploit the tumor microenvironment: passive and active tumor targeting of nanocarriers for anti-cancer drug delivery [J]. Journal of Controlled Release, 2010, 148(2): 135-146
[4]	Jansen L, Koch L, Brenner H, et al. Quality of life among long-term (≥5years) colorectal cancer survivors-systematic review [J]. European Journal of Cancer, 2010, 46(16): 2879-2888.
[5]	Wu Qu(武曲), Huo Meirong(霍美蓉), Zhou Jianping(周建平). Advances on novel drug system [J]. Journal of China Pharmaceutical University(中国药科大学学报), 2013, 44(2): 97-104
[6]	Liu Xiudong(刘袖洞), Ma Xiaojun(马小军), Yuan Quan(袁权). Drug delivery systems [J]. Journal of Chemical Industry and Engineering(China)(化工学报), 2005, 56(6): 955-961
[7]	Brewer E, Coleman J, Lowman A. Emerging technologies of polymeric nanoparticles in cancer drug delivery [J]. Journal of Nanomaterials, 2011, 2011: 1
[8]	Xu Wen(徐雯), Pan Jun(潘俊). The research progress of ligand-grafted liposomes [J]. World Clinical Drugs(国外医药: 合成药. 生化药. 制剂分册), 2002, 23(5): 293-297
[9]	Ji Junmin(纪俊敏), Xie Wenlei(谢文磊). The research progress of liposomes as a drug carrier [J]. Journal of Zhengzhou Grain College(郑州工程学院学报), 2002, 23(4): 68-72
[10]	Duan Kangying(段康颖), Wang Congrong(王从容),Li Qi(李琦). The new drug carrier — liposomes [J]. Chinese Journal of Hospital Pharmacy(中国医院药学杂志), 2010, 30(10): 864-866
[11]	Wang C Y, Hughes K W, Huang L. Improved cytoplasmic delivery to plant protoplasts via pH-sensitive liposomes [J]. Plant Physiology, 1986, 82(1): 179-184
[12]	Quan Dongqin(全东琴), Su Desen(苏德森). Studies on the preparation and characteristics of empty proliposomes as a drug carrier [J]. Journal of Shenyang Pharmaceutical University (沈阳药科大学学报), 1999, 16(3): 160-163
[13]	Jahn A, Vreeland W N, Gaitan M, et al. Controlled vesicle self-assembly in microfluidic channels with hydrodynamic focusing [J]. Journal of the American Chemical Society, 2004, 126(9): 2674-2675
[14]	Meure L A, Foster N R, Dehghani F. Conventional and dense gas techniques for the production of liposomes: a review [J]. AAPS PharmSciTech, 2008, 9(3): 798-809
[15]	Wang F, Shen Y, Xu X, et al. Selective tissue distribution and long circulation endowed by paclitaxel loaded PEGylated poly(e-caprolactone-co-l-lactide) micelles leading to improved anti-tumor effects and low systematic toxicity [J]. International Journal of Pharmaceutics, 2013, 456(1): 101-112
[16]	Chen C Y, Kim T H, Wu W C, et al. pH-dependent, thermosensitive polymeric nanocarriers for drug delivery to solid tumors [J]. Biomaterials, 2013, 34(18): 4501-4509
[17]	Huang Y, Yu H, Xiao C. pH-sensitive cationic guar gum/poly(acrylic acid) polyelectrolyte hydrogels: swelling and in vitro drug release [J]. Carbohydrate Polymers, 2007, 69(4):774-783
[18]	Pradhan P, Giri J, Banerjee R, et al. Preparation and characterization of manganese ferrite-based magnetic liposomes for hyperthermia treatment of cancer [J]. Journal of Magnetism and Magnetic Materials, 2007, 311(1): 208-215
[19]	Yang X, Li Y, Li M, et al. Hyaluronic acid-coated nanostructured lipid carriers for targeting paclitaxel to cancer [J]. Cancer Letters, 2013, 334(2): 338-345
[20]	Felber A E, Dufresne M H, Leroux J C. pH-sensitive vesicles, polymeric micelles, and nanospheres prepared with polycarboxylates [J]. Advanced Drug Delivery Reviews, 2012, 64(11): 979-992
[21]	Zhang Y, Rong Qi X, Gao Y, et al. Mechanisms of co-modified liver-targeting liposomes as gene delivery carriers based on cellular uptake and antigens inhibition effect [J]. Journal of Controlled Release, 2007, 117(2): 281-290
[22]	Mignani S, El Kazzouli S, Bousmina M, et al. Expand classical drug administration ways by emerging routes using dendrimer drug delivery systems: a concise overview [J]. Advanced Drug Delivery Review, 2013, 65(1): 1316-1310
[23]	Zhao Y, Su H, Fang L, et al. Superabsorbent hydrogels from poly (aspartic acid) with salt-, temperature- and pH-responsiveness properties [J]. Polymer, 2005, 46(14): 5368-5376
[24]	Cheng Daming(成大明), Chen Qiang(陈强), Zhu Aiping(朱爱萍), et al. Progress in research on poly( aspartic acid ) and its derivatives [J]. Materials Review(材料导报), 2007, 16(7): 60-63
[25]	Xu Yunlong(徐云龙), Jiang Houyou(姜厚友), Qian Xiuzhen(钱秀珍), Ma Xinsheng(马新胜). Preparation and properties of pH-sensitive doxorubicin nanoliposomes [J]. Journal of East China University of Science and Technology (华东理工大学学报), 2008, 34(3): 364-368
[26]	Su Haijia(苏海佳), Wang Lilin(王丽琳), Tan Tianwei(谭天伟). Preparation of a pH-sensitive modified liposomes[P]: CN, 103585106A. 2014-02-19

微酸环境响应的聚天冬氨酸修饰脂质体的制备与表征

Preparation and characterization of subacid environment responsive polyaspartic acid modified liposomes

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