F/F对嗜盐污泥以乙酸钠为底物生产PHB能力的影响

doi:10.11949/j.issn.0438-1157.20141149

化工学报 ›› 2015, Vol. 66 ›› Issue (4): 1491-1497.DOI: 10.11949/j.issn.0438-1157.20141149

F/F对嗜盐污泥以乙酸钠为底物生产PHB能力的影响

崔有为, 冀思远, 卢鹏飞, 张宏宇

北京工业大学环境与能源工程学院, 北京 100124

收稿日期:2014-07-30 修回日期:2014-12-22 出版日期:2015-04-05 发布日期:2015-04-05
通讯作者: 崔有为
作者简介:崔有为(1977-),男,博士,副教授。
基金资助:
国家自然科学基金项目(51178004,51478011);北京市自然科学基金项目(8132013);北京市教育委员会面上项目(KM201210005007)。

Influence of F/F on PHB production by halophilic activated sludge fed by sodium acetate

CUI Youwei, JI Siyuan, LU Pengfei, ZHANG Hongyu

School of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China

Received:2014-07-30 Revised:2014-12-22 Online:2015-04-05 Published:2015-04-05
Supported by:
supported by the National Natural Science Foundation of China (51178004, 51478011), the Natural Science Foundation of Beijing (8132013) and the General Program of Beijing Municipal Education Committee (KM201210005007).

摘要/Abstract

摘要：

嗜盐混合菌生产PHB具有较好的成本优势。本研究接种入海口底泥发展嗜盐活性污泥,利用嗜盐活性污泥以两步法工艺生产PHB。在连续300 d的试验中从长期试验、发酵生产PHB以及表观动力学等方面探索了不同盛宴期/饥饿期(F/F)下筛选菌群的PHB积累能力。研究发现F/F作为重要的参数直接影响嗜盐活性污泥的PHB积累能力。在高F/F下非PHB积累菌群成为优势菌,而低F/F下促进高聚PHB嗜盐菌的选择和富集。在F/F≤0.33条件下筛选的嗜盐活性污泥最大PHB含量可以达到细胞干重的46.7%,PHB产率为0.358 mg PHB·(mg Ac)^-1。研究结果将对进一步实现嗜盐污泥生产PHB提供有益的指导。

关键词: 聚-b-羟基脂肪酸酯, 嗜盐活性污泥, 两步法, PHA合成菌, 菌群选择, 生物过程, 生物技术, 数值分析

Abstract:

Poly-b -hydroxybutyrate (PHB) production by halophilic mixed consortia has great advantage in cost. PHB production by the two-step production process using halophilic activated sludge cultured from estuary sediment was investigated. During the continuous 300 d, the ability of PHB storage at different F/F was assessed from the performance of the long-term tests, experiments of PHB production and the observed kinetics. The ratio of feast to famine (F/F) as an important parameter directly affected the capacity of the PHB-storing of halophilic activated sludge. At a high F/F the non PHB-storing bacteria became dominant in the enrichment reactor, while a low F/F was in favor of the selection and enrichment of PHB-storing bacteria. Moreover, the culture selected at F/F less than 0.33 showed the best PHB-storing capacity with a PHB content of 46.7% cell dry weight and a PHB yield of 0.358 mg PHB·(mg Ac)^-1. The results obtained in the study provided a guidance to the production of PHB by halophilic activated sludge.

Key words: poly-b-hydroxybutyrate (PHB), halophilic activated sludge, two-step production process, PHA- storing bacteria, culture selection, bioprocess, biotechnology, numerical analysis

中图分类号:

X703

崔有为, 冀思远, 卢鹏飞, 张宏宇. F/F对嗜盐污泥以乙酸钠为底物生产PHB能力的影响[J]. 化工学报, 2015, 66(4): 1491-1497.

CUI Youwei, JI Siyuan, LU Pengfei, ZHANG Hongyu. Influence of F/F on PHB production by halophilic activated sludge fed by sodium acetate[J]. CIESC Journal, 2015, 66(4): 1491-1497.

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F/F对嗜盐污泥以乙酸钠为底物生产PHB能力的影响

Influence of F/F on PHB production by halophilic activated sludge fed by sodium acetate

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